Disruption of global hypothalamic microRNA (miR) profiles and associated behavioral changes in California mice (Peromyscus californicus) developmentally exposed to endocrine disrupting chemicals
»
Disruption of global hypothalamic microRNA (miR) profiles and associated behavioral changes in California mice (Peromyscus californicus) developmentally exposed to endocrine disrupting chemicals
Development exposure to the disturbance of endocrine disturbance chemicals (EDCS), for example, bisphenol A (BPA) or Genistein (GEN), causes long-standing epigenomal effects. Micronas (Mirs) Adjust the MRNAS to be translated into protein and thus serve as a final checkpoint in epigenetic control. The safer amount is, however, known if the EDCs affect Mirna (MIR) neuron models. We aim to test the hypothesis that the exposure to the development of California mice with Gen, BPA or the two chemicals influenced the hypothalamamic profiles MIR / SMALL RNA and verifies the measurement of these biomolecular alterations in correlation of Behavioral and metabolic changes. California mice have been developed for Gen development, GEN + BPA, low dose ba, or upper BPA dose.
Adult offspring has been tested in a battery of behavioral and metabolic tests; On this, the mice were euthanized and frozen brains collected, small RNAs were isolated hypothalamic punches and subsequent RNA sequencing. California mice exposed to one or both EDCs are engaged in one or more repetitive behaviors. GEN, LD BPA and UD BPA modified aspects of ultrasonic and audible vocalizations. Each EDC exposure has led to gender-dependent differences in sex-expressed RNAs with MIR7-2, MIR146 and MIR148A being increased in all exposed groups of all women and masculine EDC. The current results reveal that the exposure to the development of Gen and / or BPA affects hypothalamic mir / small RNA RNA expression models, as well as such changes correlated with EDC-induced behavioral and metabolic alterations. MIR146 is probably an important mediator and an EDC exposure biomarker in mammals, including humans.
Aging is a critical risk factor for chronic diseases of the elderly. Micronas regulates the silent post-transcriptional gene through a bond of the basic pair on their target mnas. We have identified nonlinear changes in age-related microarnas by analyzing the total blood of 1334 healthy people. We observed a greater influence of age compared to sex and provide evidence of a passage to the form of 5 ‘mirnas in good health. The addition of 3059 patients discovered pan-disease disease and specific disease modifications in aging profiles. DISEASE Biomarker sets for all diseases were different between young and old patients.
Integrated analysis of microarna and mRNA expression profiles in Crassostre gigas to reveal the pigmentation of the Mirna and Mirna-Mirna shell
Micronas (MIRNAS) Adjust the post-transcription gene expression by targeting genes and play crucial roles in various biological processes involving body color training. However, mirnas and mirna targets underlying the polymorphism of shell colors remain largely unknown in Molluscla. Using four colors of SIB families’ colors in the pacific oyster Crassostrea Gigas, we have systematically identified mirnas and Mina targets in the coats, that the organ could produce a white, gold, black shell or partially pigmented.
The sequencing and analysis of RNA identified a total of 53 Known Mirna and 91 new MIRNAS, of which 47 were detected to express differently among six pair groups. By integrating MIRNA and mRNA expression profiles, a total of 870 genes have been predicted as differentially expressed mirnas objectives, mainly involving in biomineralization and pigmentation by functional enrichment. In addition, a total of four mirnas and their target RNAs have been planned to involve melanin, carotenoid or tetapyrrole synthesis.
Of them, LGI-MIR-317 and its peroxidase and lncra targets TCons_00951105 are involved by acting as the competitive endogenous RNA to regulate melanogenesis. Our studies have revealed the systematic characterization of Mirnas profiles expressed in the oyster mantle, which could facilitate the understanding of the complex molecular regulation of the polymorphism of the colors of shell and to provide new perspectives in the search for reproduction. Oyster.
Disruption of global hypothalamic microRNA (miR) profiles and associated behavioral changes in California mice (Peromyscus californicus) developmentally exposed to endocrine disrupting chemicals
Profile Microna of cardiovascular risk in patients with obstructive sleep apnea
Background: Obstructive sleep apnea (OSA) is a common illness caused by repeated collapse episodes of upper respiratory tract during sleep and is associated with the development of cardiovascular disease (CVD). However, there is a high heterogeneity in the impact of the OSA on patients. So far, the profile of OSA patients at CVD’s risk of development has not been defined, including measurable variables that can be used to predict the risk of a patient with OSA patient.
Objective: The objective of this study was to identify the profile Microna (MI-RNA) associated with the CVD in patients with OSA.
Method: This is an observational joint interview interview that included 132 male patients. Three groups have been defined as patients with OSA, patients with OSA with Hypertension and OSA patients who have developed a major cardiovascular event. Polysomnography and measurements of ambulatory blood pressure were performed. The expression profiling of 188 plasma mirnas was carried out in 21 subjects (corresponding by IMC and age) by the Taqman low density table (TLDA). MIRNAS Differently expressed in the various subgroups of patients and mirnas who correlated with the cardiovascular risk score were selected for RT-QPCR validation in the remaining 111 patients.
Results: From the TLDA analysis, 7 MIRNAS have been selected for validation. The differential expression has been confirmed in any of the mirnas. MIR-143 was associated with night systolic blood pressure. MIR-107 correlated with 24-H arterial pressure parameters and with night hypertension. MIR-486 was associated with the cardiovascular risk score.
Conclusions: The MIRNAS traffic profile does not appear to be different in any of the subgroups of patients with different OSA and different cardiovascular risk factors. Nevertheless, MIR-107 and MIR-143 are associated with specific arterial pressure parameters in patients with OSA and MIR-486 are associated with the cardiovascular risk score.
