Serum-Derived Exosomal MicroRNA Profiles Can Predict Poor Survival Outcomes in Patients with Extranodal Natural Killer/T-Cell Lymphoma
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Serum-Derived Exosomal MicroRNA Profiles Can Predict Poor Survival Outcomes in Patients with Extranodal Natural Killer/T-Cell Lymphoma
Exosomes containing microarnas (MIRNAS) may have a utility such as biomarkers to predict the risk of treatment failure in the extraranodal nk / T-cell lymphoma (ENKTL) because the exosomal cargo mirnas could reflect tumor aggressiveness. We analyzed the exosomal mirnas of the patients favorable (n = 22) and the new results (n = 23) in a training cohort. Then, using the Nanostring NCOUNTER® Microna matrix, we compared them with miracas identified in exosomes derived from the human NK / T lymphoma chain (NKTL) to develop exosomal Mije profiles. We validated the prognostic value of the serum exosomal mina profiles with an independent cohort (n = 85) and analyzed their association with treatment resistance using stubborn resistant cell lines.
Comparison of the 20 best mirnas regulated in the cohort of training with mediocre results with 16 MIRNAS that have been regulated in both NKTL cell lines, have identified five MIRNAS candidates (MIR-320E, MIR-4454, MIR-222-3P, MIR-21- 5P and MIR-25-3P). Among these, an increase in the exosomal levels mir-4454, MIR-21-5P and MIR-320E were associated with low overall survival in the validation cohort. Increased levels have also been found in post-treatment relapsed patients.
These three mirnas have been overexpressed in NKTL cell lines resistant to etoposide. In addition, the transfection of NKTL cell lines with MIR-21-5P and MIR-320E resulted in an increase in the expression of preflammatory cytokines such as the alpha macrophages inflammatory protein. These studies show that serum exosomal levels MIR-21-5P, MIR-320E and MIR-4454 are increased in Enktl patients with a bad prognosis. The regulation of these exosomal miracas in the resistant cell lines suggests that they have a biomarker role for the identification of Enktl patients at high risk failure.
Trypanosoma Cruzi and Toxoplasma Gondii induce a differential micro-microorna profile in Pestal Human Explants
Trypanosoma Cruzi and Toxoplasma Goniii are two parasites that can be transmitted from mother to the child through the placenta. However, congenital transmission rates are low for T. cruzi and high for T. gondiii. The success or failure of the infection depends on the complex-host interactions in which the parasites can alter the expression of host genes by modulating non-coding RNA such as MIRNAS. To date, there are still no reports on the expression of Mirna altered in the placental tissues in response to each parasite. As a result, we infected ex-vivo placental explants by cultivation with T. cruzi or T. gondii for 2 h.
We then analyzed the Mirna expression profiles of the two types of fabrics infected with the sequencing of Mirna and the quantitative PCR, the targeted forecast of the target of the sequence, the functional enrichment of the channel and the analysis of the regulator Upstream of the genes expressed differentially targeted by mirnas expressed differently. The two parasites have induced specific Minna profiles. Go Analysis revealed that the predicted silico targets expressed differentially have set different cell processes involved in development and immunity, and most identified KEGG channels were related to chronic diseases and infection.
Considering that the mirnas expressed differentially identified here modulated crucial cell targets that participate in the determination of the success of the infection, these mirnas could explain the different congenital transmission rates between the two parasites. The molecules of the different routes regulated by mushas and modulated during the infection, as well as the mirnas themselves, can be potential targets for the therapeutic control of congenital chagas disease or toxoplasmosis.
Serum-Derived Exosomal MicroRNA Profiles Can Predict Poor Survival Outcomes in Patients with Extranodal Natural Killer/T-Cell Lymphoma
Exploration of Microrna profiles in human colostrum
Background: Colostrum is well known for having excellent nutritional value for newborns. The objective of this study was to study the dynamic expression scheme of microarna in human colostrum and mature milk. In addition, we have identified the specific microorna in human colostrum and analyzed the regulatory function of human colostrum.
Methods: We collected breast milk samples from 18 wholly volunteers. The expression of microorna in breast milk was detected by analysis of the chip. The expression differences were characterized by Log2FC (| Log2Fold Change |> 1.58) and associated p values (p <0.05). In addition, the anticipation of the microad objectives, a bioinformatics analysis and a generation of networks have been carried out using the network database.
Results: Our results have shown that during the human lactation process, the composition of microarnas in human milk changes dynamically. Compared to the microorna expression profile in human mature milk, the expression levels of 49 micrrands were clearly different and 67 micrrands were specifically expressed in human colostrum. Based on the results of Gene Ontology (GB) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Analysis of Genome Lane Enrichment, the planned target RNNA of identified colostrum microarnas has been involved in the regulations. Separate biological processes, such as signal transduction, positive regulation of GTPASE activity and protein phosphorylation. In addition, the planned mRNA targets came from large signaling path spectra, such as MAPK, RAS, Hippo, WNT and MTOR signaling pathways, as well as the lane for longevity regulation.
Description: microRNA-21-IN-2 is a potential miR-21 inhibitor with an AC50 value of 3.29 μM. microRNA-21-IN-2 can be used for the research of cancer[1].
Description: microRNA-21-IN-3 (compound 45) can specifically bind to the precursor of oncogenic and pro-inflammatory microRNA-21 with medium nanomolar affinity, reduce cancer cell proliferation and miR-21 levels, and can be used in antitumor research[1].
Description: microRNA-21-IN-1 (compound 7A) is an efficient microRNA inhibitor. microRNA-21-IN-1 has antiproliferative activity against Hela and HCT-116 cells with IC50s of 5.5 μM and 2.8 μM respectively, as well as promotes apoptosis of Hela cells. microRNA-21-IN-1 upregulates the expression of microRNA-21 downstream functional targets (PTEN, EGR1 and SLIT2). microRNA-21-IN-1 can be used for researching anticancer[1].
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Bromodomain testis specific protein(BRDT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Bromodomain testis specific protein(BRDT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Bromodomain testis specific protein(BRDT) ELISA kit
Conclusions: Our study illuminates the landscape of microornished expressions in human colostrum and mature milk, and highlights the value of micronas as nutritional additives in commercial products related to milk.
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