Proteomic and microRNA-omic profiles and potential mechanisms of dysfunction in pancreatic islet cells primed by inflammation
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Proteomic and microRNA-omic profiles and potential mechanisms of dysfunction in pancreatic islet cells primed by inflammation
Diabetes is an inflammatory disease that induces the dysfunction of pancreatic islands. However, at best our knowledge, the potential underlying molecular mechanisms of this inflammatory process remain unknown. This study investigated microarna expression profiles (MIRNA / MIR) and protein through proteomics and mIRNA-OMICS. The lipopolysaccharide macrophage cellular medium (LRM) was used to stimulate the inflammation of Beta-TC-6 mouse island cells. Protein analysis revealed that 87 proteins were regulated and 42 proteins were regulated in the BETA-TC-6 cells treated with the LRR with respect to control cells.
In addition, MIRNA’s analysis revealed that 11 miRNas were regulated, while 28 miRNas were carried out in the Beta-TC-6 cells treated with the LRR with respect to control cells. The island cells exposed to inflammation have presented significant transcription protein levels of the MAFA transcription factor, pancreatic and duodenal homeobox 1, panel 6, HomeOBOX NKX-2.2 protein, protein associated with synaptosomale 25, glucagon and Insulin-2, while the expression of MIR-146A -5P and MIR-21A-5P have been regulated. It has also been determined that utility levels MIR-146A-5P and MIR-21A-5P can be mediated by NF-κB activation. The regulation of the decline of the Islet Factor Factor MRNA has been partially reversed by treating island cells with a MIR-21A-5P inhibitor. However, treatment with a mir-146a-5p inhibitor has not exercised the same effect.
Overall, the present study determined the molecular profiles of the cellular ignition of islets based on proteomics and mIRNA-OMICS, and indicated that proteins and mirnas with modified expressions can form a large network that serves as a role in The dysfunction of islets. In particular, the regulation of MIR-21A-5P in response to inflammation can contribute to the malfunction of the ist cells. However, how these mushas have settled the expression of some RNAs and proteins in the inflammation of ist cells requires further investigation.
The ancient microornas profiles of a sample of 14,300 years Canid confirm the taxonomic origin and give insights in the regulation of specific genes of the pleistocene tissues
The sequencing of the DNA is the current key technology of historical or old biological samples and has led to many exciting discoveries in the field of paleoganomics. However, functional information on tissue identity, cell composition or gene regulation can not be won from the DNA. Recent analyzes have shown that, under favorable conditions, RNA can also be sequenced by ancient samples, allowing studies at transcriptomic and regulatory level. Analysis of ancient RNA data from a Pleistocene Canid, we find hundreds of intact micro portions that are taxonomic informative, have a specificity of tissues and have functionally predictive characteristics. With an extraordinary age of 14,300 years, these microad sequences are by far the oldest ever reported.
The authenticity of the sequences is further supported by a) the presence of sequences specific to Canid / craniform which has never evolved outside this clade, b) patterns of expression specific to a fabric (cartilage, liver and muscle) that resemble those of modern dogs and c) RNA damage models that are clearly distinct from fresh samples. By performing enrichment analyzes of the IT micro-merchandemic target on ancient sequences, we predict the functions of the micro-lead compatible with their tissue expression model.
For example, we find a liver-specific Microrna that regulates metabolism of carbohydrates and famine responses in the canids. In summary, we show that simple micro-micro-microornaomicscriptomic analyzes can give functional insights in tissue identity, the cellular composition and the regulatory activity of the old samples and the biological processes that took place in the pleistocene, Thus holding a great promise for deeper ideas in the regulation of genes in animals off extinct animals. Based on the old sequencing of RNA.
Proteomic and microRNA-omic profiles and potential mechanisms of dysfunction in pancreatic islet cells primed by inflammation
Exosomes derived from the human periodontal ligament cell promote bone regeneration by modifying microad profiles
The role and underlying mechanism of exosomes derived from human periodontal ligament stem cells (PDLSC) in osteogenesis are unclear. In this study, we identified PDLSC’s derived exosomes and found that osteogenic induction can improve the osteogenic capacity of PDLSC derived exosomes in promoting osteogenic differentiation of rat bone bone marrow stem cells (BMSCS). To study the underlying mechanism, we analyzed the exosomal expression profiles of Mirna of the infronted and osteogenic differentiated differentiated PDLSC by sequencing RNA. The results showed that seventy-two mirnas were regulated and thirty-five mirnas were regulated after osteogenic induction.
The results of the analysis of the ontology of genes and the analysis of the channels have shown that the target genes of exosotional exosomal miras in differential way participate in the regulation of various biological processes, such as catalytic activity, the connection protein, metabolic processes, cell development and differentiation, and are enriched in differentiation-related osteogenic differentiation routes, such as MAPK signaling, AMPK signaling and insulin signaling pathways. Our results reveal for the first time that exosomal miracas derived from Osteogenic differentiated PDLSCs can promote Osteogenic Differentiation of BMSCs, which provides a basis for new research on the regulatory function of the exosomal Mijonde of PDLSCs during osteogenesis.
