Proteomic and microRNA-omic profiles and potential mechanisms of dysfunction in pancreatic islet cells primed by inflammation
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Proteomic and microRNA-omic profiles and potential mechanisms of dysfunction in pancreatic islet cells primed by inflammation
Diabetes is an inflammatory disease that induces the dysfunction of pancreatic islands. However, at best our knowledge, the potential underlying molecular mechanisms of this inflammatory process remain unknown. This study investigated microarna expression profiles (MIRNA / MIR) and protein through proteomics and mIRNA-OMICS. The lipopolysaccharide macrophage cellular medium (LRM) was used to stimulate the inflammation of Beta-TC-6 mouse island cells. Protein analysis revealed that 87 proteins were regulated and 42 proteins were regulated in the BETA-TC-6 cells treated with the LRR with respect to control cells.
In addition, MIRNA’s analysis revealed that 11 miRNas were regulated, while 28 miRNas were carried out in the Beta-TC-6 cells treated with the LRR with respect to control cells. The island cells exposed to inflammation have presented significant transcription protein levels of the MAFA transcription factor, pancreatic and duodenal homeobox 1, panel 6, HomeOBOX NKX-2.2 protein, protein associated with synaptosomale 25, glucagon and Insulin-2, while the expression of MIR-146A -5P and MIR-21A-5P have been regulated. It has also been determined that utility levels MIR-146A-5P and MIR-21A-5P can be mediated by NF-κB activation. The regulation of the decline of the Islet Factor Factor MRNA has been partially reversed by treating island cells with a MIR-21A-5P inhibitor. However, treatment with a mir-146a-5p inhibitor has not exercised the same effect.
Overall, the present study determined the molecular profiles of the cellular ignition of islets based on proteomics and mIRNA-OMICS, and indicated that proteins and mirnas with modified expressions can form a large network that serves as a role in The dysfunction of islets. In particular, the regulation of MIR-21A-5P in response to inflammation can contribute to the malfunction of the ist cells. However, how these mushas have settled the expression of some RNAs and proteins in the inflammation of ist cells requires further investigation.
The ancient microornas profiles of a sample of 14,300 years Canid confirm the taxonomic origin and give insights in the regulation of specific genes of the pleistocene tissues
The sequencing of the DNA is the current key technology of historical or old biological samples and has led to many exciting discoveries in the field of paleoganomics. However, functional information on tissue identity, cell composition or gene regulation can not be won from the DNA. Recent analyzes have shown that, under favorable conditions, RNA can also be sequenced by ancient samples, allowing studies at transcriptomic and regulatory level. Analysis of ancient RNA data from a Pleistocene Canid, we find hundreds of intact micro portions that are taxonomic informative, have a specificity of tissues and have functionally predictive characteristics. With an extraordinary age of 14,300 years, these microad sequences are by far the oldest ever reported.
The authenticity of the sequences is further supported by a) the presence of sequences specific to Canid / craniform which has never evolved outside this clade, b) patterns of expression specific to a fabric (cartilage, liver and muscle) that resemble those of modern dogs and c) RNA damage models that are clearly distinct from fresh samples. By performing enrichment analyzes of the IT micro-merchandemic target on ancient sequences, we predict the functions of the micro-lead compatible with their tissue expression model.
For example, we find a liver-specific Microrna that regulates metabolism of carbohydrates and famine responses in the canids. In summary, we show that simple micro-micro-microornaomicscriptomic analyzes can give functional insights in tissue identity, the cellular composition and the regulatory activity of the old samples and the biological processes that took place in the pleistocene, Thus holding a great promise for deeper ideas in the regulation of genes in animals off extinct animals. Based on the old sequencing of RNA.
Proteomic and microRNA-omic profiles and potential mechanisms of dysfunction in pancreatic islet cells primed by inflammation
Exosomes derived from the human periodontal ligament cell promote bone regeneration by modifying microad profiles
The role and underlying mechanism of exosomes derived from human periodontal ligament stem cells (PDLSC) in osteogenesis are unclear. In this study, we identified PDLSC’s derived exosomes and found that osteogenic induction can improve the osteogenic capacity of PDLSC derived exosomes in promoting osteogenic differentiation of rat bone bone marrow stem cells (BMSCS). To study the underlying mechanism, we analyzed the exosomal expression profiles of Mirna of the infronted and osteogenic differentiated differentiated PDLSC by sequencing RNA. The results showed that seventy-two mirnas were regulated and thirty-five mirnas were regulated after osteogenic induction.
Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) Antibody
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Porcine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELI
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Troponin C, slow skeletal and cardiac muscles in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Bovine skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Monkey (Cynomolgus) skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
Description: human skeletal muscle tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
The results of the analysis of the ontology of genes and the analysis of the channels have shown that the target genes of exosotional exosomal miras in differential way participate in the regulation of various biological processes, such as catalytic activity, the connection protein, metabolic processes, cell development and differentiation, and are enriched in differentiation-related osteogenic differentiation routes, such as MAPK signaling, AMPK signaling and insulin signaling pathways. Our results reveal for the first time that exosomal miracas derived from Osteogenic differentiated PDLSCs can promote Osteogenic Differentiation of BMSCs, which provides a basis for new research on the regulatory function of the exosomal Mijonde of PDLSCs during osteogenesis.
