Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.

Vaccines with live Newcastle, low virulence (NDV) virus strains are still accepted prevention and control strategies for the fight against Newcastle disease (ND), a major viral disease hinders the development of the poultry industry around the world. However, the underlying mechanism for innate immune responses in the innate cell of the vaccine is unclear. Here, a broadband Illumona sequencing approach has been used to determine the expression profiles of cellular mina in lasota-infected chicken macrophages, a vaccine strain widely used for mass vaccination programs against nd in poultry.

Compared to the control group, 112 and 115 expressed differentially (de) Mirnas have been identified at 24 HPI (hours after inoculation) and 48 HPI, respectively. Meanwhile, 174 Mirnas were identified between 24 HPI and 48 HPI. In addition, 12 regulated and 6 MIRNAS were observed at 24 and 48 HPI compared to 0 HPI. In addition, the target prediction and functional analysis of these mirnas revealed a significant enrichment for several signaling pathways, particularly in genes and routes related to the immune, such as the signaling path of the RIG type receiver. -II-LIKE-I-like, a signaling channel of the Kinase Protein (MAPK) (MAPK) (MAPK) signaling receiver. Our conclusions not only have the foundations base for more research the roles and mechanisms of MIRNA regulatory in immunized immune responses mediated by vaccination, but also extend new perspectives in the interactions between the army and the NDV infection. To determine the microarna expression profile (MIRNA) in the mononuclear cells of the peripheral blood (PBMC) and immunized factors in pregnant women with hepatitis B virus infection (VHB).

A total of 182 pregnant women infected with HBV were randomly selected, 40 pregnant pregnant in good health women and 35 non-pregnant women as controls. High speed sequencing has been used to detect RNA in the PBMC of all subjects. The indirect ELISA method has been used to determine cytokine changes in peripheral blood samples. Comparked with the control group, 18 MARN expressed differentially have been identified in those with HBV infection (P <0, 01).

Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.
Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.

 

Clinical and molecular analysis of osteosarcoma associated with pathological fractures: the micro-portion profile is different and is correlated with prognosis.

The micrarnas are small non-coding RNAs that govern the expression of post-procedures genes. The presence of certain specific microarnas has been associated with an increased risk of local recurrence and metastasis and worse survival in patients with osteosarcoma. Pathological fractures in osteosarcoma are considered more the manifestation of a neoplasm with more aggressive biological behavior than the cause itself of the worst prognosis. However, this has not been proven at the biological or molecular level. Currently, there has been no microna profiling study of patients with osteosarcoma with and without pathological fractures that have described differences in microorna profiling between these two groups and their correlation with biological behavior.

(1) In patients with osteosarcoma extremities, how do the profiles of micro-portions thereof with and without pathological fractures do they compare? (2) What relations have microarnas with local recurrence, the risk of metastases, the survival specific to a disease and overall survival of patients with osteosarcoma with pathological fractures? Between 1994 and 2013, 217 patients were diagnosed and treated in our institution for osteosarcoma extremities. Patients were excluded if (1) they suffered oncolological resection of osteosarcoma in an external institution (two patients) or (2) they were diagnosed with an osteosarcoma extras (29 patients) or (3), They had less than a year of clinical follow-up -up and no oncolological result (local recurrence, metastasis or death) (four patients). In total, 182 patients were eligible. Of these 143 were high quality osteosarcomas. After the evaluation of tumor samples before the treatment of chemotherapy, a total of 80 consecutive samples were selected for sequencing. The demographic and clinical comparison between sequenced and non-sequenced patients has demonstrated no difference, confirming that both groups were comparable.

Diagnostic samples of the 80 patients with high-quality end osteosarcomas that had not yet received chemotherapy undergoes micro-microorna sequencing for a large-scale osteosarcoma genome profiling project in our institution. Six samples were removed after a second aspect by a musculoskeletal pathologist who verified the celluliarity and the quality of the samples to be sequenced, leaving a total of 74 patients. Of these, two samples have been removed because they have been confirmed to be pelvic tumors in a second check after sequencing. The final sample of the study was 72 patients (11 patients with pathological fractures and 61 without). The sequencing data has been correlated with local fractures and recurrence, a risk of metastases, survival specific to overall disease and survival through Kaplan-Meier.seral Micronas analyzes has been expressed differently between the two groups. Among the markers with the highest differential expression (Edger and DESEQ algorithms), and HSA-MIR 381-3P have been regulated in patients with pathological fractures, while that have been regulated.

miRNA All-In-One cDNA Synthesis Kit

G898 20 rxn
EUR 180
Description: miRNA All-In-One cDNA Synthesis Kit is a complete system for efficient synthesis of first strand miRNA from total RNA templates. This comprehensive kit comes with just two components: 2X miRNA cDNA Synthesis SuperMix and Enzyme Mix, enabling hassle-free set-ups with minimal potential for contamination due to significantly reduced pipetting. The kit contains all miRNA synthesis-required reagents including Poly(A) Polymerase and abm’s OneScript® Plus Reverse Transcriptase, a unique miRNA Oligo d(T) adapter that anneals to the A-tailed miRNA template, and RNaseOFF Ribonuclease Inhibitor to protect RNA template from degradation.

RScript™ miRNA cDNA Synthesis Kit (Poly-A)

RK005-0050 50 rxns
EUR 430

RScript™ miRNA cDNA Synthesis Kit (Stem-loop)

RK004-0050 50 rxns
EUR 65

miRNA 1st Strand cDNA Synthesis Kit (by tailing A)

MR201-01 20 rxns (20 µl/rxn)
EUR 145

miRNA 1st Strand cDNA Synthesis Kit (by tailing A)

MR201-02 50 rxns (20 µl/rxn)
EUR 304.5

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

MR101-01 50 rxn (20 μl/rxn)
EUR 56.26

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

MR101-02 100 rxn (20 μl/rxn)
EUR 108.5

miRNA First-Strand cDNA Synthesis SuperMix

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miRNA First-Strand cDNA Synthesis SuperMix

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miRNA First-Strand cDNA Synthesis SuperMix

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cDNA Synthesis Kit

abx460012-1096tests 10 × 96 tests Ask for price

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abx460012-596tests 5 × 96 tests Ask for price

cDNA Synthesis Kit

abx460012-96tests 96 tests
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cDNA Synthesis Kit

abx461055-1096tests 10 × 96 tests Ask for price

cDNA Synthesis Kit

abx461055-596tests 5 × 96 tests
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cDNA Synthesis Kit

abx461055-96tests 96 tests
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cDNA Synthesis Kit

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cDNA Synthesis Kit

abx461073-596tests 5 × 96 tests
EUR 1037.5

cDNA Synthesis Kit

abx461073-96tests 96 tests
EUR 525

EVery cDNA Synthesis Kit

EVery200B-1 20 preps
EUR 307

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SCRIPT cDNA Synthesis Kit

PCR-511L 500reactions x 20 µl
EUR 1349.2

SCRIPT cDNA Synthesis Kit

PCR-511S 100reactions x 20 µl
EUR 337.3

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 370.8

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

Viva cDNA Synthesis Kit, 100app

cDSK01-100 each Ask for price

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 116.4

Viva cDNA Synthesis Kit, 50app

cDSK01-050 each Ask for price

RScript™ cDNA Synthesis Kit

RK001-0050 50 rxns
EUR 60

OneScript Plus cDNA Synthesis Kit

E36G236 100 rxn
EUR 207.14

OneScript Plus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 195
Description: OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates.

EpiNext Hi-Fi cDNA Synthesis Kit  

P-9004
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  • 20 Samples
  • 20 Reactions

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 268.8

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 652.8

OneScript Hot RT cDNA Synthesis Kit

E36G594 100 rxn
EUR 271.43

OneScript Hot RT cDNA Synthesis Kit

G594 100 rxn
EUR 295
Description: OneScript® Hot cDNA Synthesis Kit is the first kit in the world that allows for efficient androbust cDNA synthesis at 60ºC. All other cDNA synthesis kits on the market are optimizedfor the reaction to be performed at 42-55ºC, which is sufficient for most RNA template, butfails on more difficult templates. abm is the only company in the world to have a reversetranscriptase engineered to offer superior cDNA synthesis performance with even the mostchallenging RNA samples due to its incredible thermostability at 60-72ºC. The enzyme is amutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that canreverse transcribe low abundance or degraded RNA, and has significantly better resistanceto contaminating inhibitors such as reagents used during RNA extraction and contaminantsfrom biological samples. High processivity and sensitivity allow for rapid cDNA synthesis offull-length cDNA fragments in a fraction of the time of leading competitors.This kit provides a comprehensive set of reagents necessary to generate high quality cDNAand offers the most flexibility in respect to priming methods and reaction optimization. Bothrandom primers and oligo(dT) are included for a choice of general priming strategies andas alternatives to gene-specific primers.

cDNA Synthesis SuperMix

20-abx09801420ulSystems
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cDNA Synthesis SuperMix

abx098014-100l 100 µl
EUR 725

cDNA Synthesis SuperMix

abx098014-1ml 1 ml Ask for price

cDNA Synthesis SuperMix

abx098014-200l 200 µl
EUR 812.5

RScript™ cDNA Synthesis Kit (Premix)

RK002-0050 50 rxns
EUR 60

AzuraQuant cDNA Synthesis Kit - 100 Reactions

AZ-1996 100 Reactions
EUR 446.4

Optimax First Strand cDNA Synthesis Kit

K4201100 100 rnx
EUR 466

HiFiScript 1st Strand cDNA Synthesis Kit

W2569-10 10 rxn
EUR 139

HiFiScript 1st Strand cDNA Synthesis Kit

W2569-30 30 rxn
EUR 399

HiFiScript 1st Strand cDNA Synthesis Kit

W2569-100 - Ask for price

AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 183.6

Accuris qMax cDNA Synthesis Kit, 100 reactions

PR2100-C-100 1 PC
EUR 443.72

Accuris qMax cDNA Synthesis Kit, 250 reactions

PR2100-C-250 1 PC
EUR 1052.25

M-MuLV First Strand cDNA Synthesis Kit

K5147-100Rxns 100Rxns
EUR 338.8
Description: Biotechnology

HyperScript First-Strand cDNA Synthesis Kit

K1072-100 100 rxn (20 uL/rxn)
EUR 246
Description: Synthesize first-strand cDNA from purified poly(A)+ or total RNA

HyperScript First-Strand cDNA Synthesis Kit

K1072-20 20 rxn (20 uL/rxn)
EUR 56
Description: Synthesize first-strand cDNA from purified poly(A)+ or total RNA

HyperScript First-Strand cDNA Synthesis Kit

K1072-50 50 rxn (20 uL/rxn)
EUR 131
Description: Synthesize first-strand cDNA from purified poly(A)+ or total RNA

HiScript II 1st Strand cDNA Synthesis Kit

R211-01 50 rxn (20 μl/rxn)
EUR 88.61

HiScript II 1st Strand cDNA Synthesis Kit

R211-02 100 rxn (20 μl/rxn)
EUR 159.5

RNA-Quant cDNA synthesis Kit (20 reactions)

RA430A-1 20 reactions
EUR 619

Accuris qMax cDNA Synthesis Kit, 25 reactions

PR2100-C-25 1 PC
EUR 126.07

Second Strand cDNA Synthesis Kit-dNTP based

G475 25 x 50 ul reactions
EUR 466.8

RScript™ cDNA Synthesis Kit (Premix + gDNA Remover)

RK003-0050 50 rxns
EUR 80

ABScript II cDNA First-Strand Synthesis Kit

RK20400 50RXN
EUR 290

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
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cDNA Synthesis SuperMix for qPCR

abx098019-100l 100 µl
EUR 825

cDNA Synthesis SuperMix for qPCR

abx098019-1ml 1 ml
EUR 2387.5

cDNA Synthesis SuperMix for qPCR

abx098019-200l 200 µl
EUR 1000

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
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Accuris qMax cDNA Synthesis Kit, sample, 5 reactions

PR2100-C-S 1 PC
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amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
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amfiRivet cDNA Synthesis 2X Buffer

R5102-100 2x500ul
EUR 230.4

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based

G476 25 x 50 ul reactions
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Hi-cDNA Synthesis Kit

MBT076-100R 1 unit
EUR 211.79
Description: Hi-cDNA Synthesis Kit

Hi-cDNA Synthesis Kit

MBT076-10R 1 unit
EUR 28.74
Description: Hi-cDNA Synthesis Kit

Hi-cDNA Synthesis Kit

MBT076-25R 1 unit
EUR 63.13
Description: Hi-cDNA Synthesis Kit

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 498

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 1016.4

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1453.2

igScript™ First Strand cDNA Synthesis Kit - 100 Reactions

4314 1/EA
EUR 350
Description: igScript™ first strand cDNA synthesis kit includes 5x igScript™ master mix which contains igScript™ Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. igScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55 ºC.Product Includes:5x igScript™ master mixOligo d(T)23 VN primer (50 µM)Random hexamer primer mix (60 µM)Nuclease free water

HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R212-01 50rxns(20µl/rxn)
EUR 108.6

HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R212-02 100rxns(20µl/rxn)
EUR 181

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-560 20 rcs
EUR 122.4

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-562 100 rcs
EUR 362.4

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-564 1000 rcs
EUR 2718

EntiLink™ 1st Strand cDNA Synthesis Kit (+gDNA Eraser)

EQ003 50 rxn
EUR 140

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R312-01 50 rxn (20 μl/rxn)
EUR 162.98

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R312-02 100 rxn (20 μl/rxn)
EUR 239

igScript™ First Strand cDNA Synthesis Kit - 25 Reactions

4312 1/EA
EUR 110
Description: igScript™ first strand cDNA synthesis kit includes 5x igScript™ master mix which contains igScript™ Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. igScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55 ºC.Product Includes:5x igScript™ master mixOligo d(T)23 VN primer (50 µM)Random hexamer primer mix (60 µM)Nuclease free water

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

amfiRivert II cDNA Synthesis Master Mix

R5500-050 50 rxns
EUR 448.8

amfiRivert II cDNA Synthesis Master Mix

R5500-100 2x50rxns
EUR 692.4

amfiRivert II cDNA Synthesis Master Mix

R5500-500 10x50rxns
EUR 1410

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
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  • 100 rxns × 20 ul Systems
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amfiRivert cDNA Synthesis Prime Master Mix

R5610-050 50 rxns
EUR 494.4

amfiRivert cDNA Synthesis Prime Master Mix

R5610-100 2x50 rxns
EUR 805.2

amfiRivert cDNA Synthesis Prime Master Mix

R5610-200 4X50 rxns
EUR 1453.2

amfiRivert cDNA Synthesis Prime Master Mix

R5610-500 10x50 rxns
EUR 3057.6

Fly First-Strand cDNA Synthesis SuperMix

abx098858-100l 100 µl
EUR 850

Fly First-Strand cDNA Synthesis SuperMix

abx098858-1ml 1 ml Ask for price

Fly First-Strand cDNA Synthesis SuperMix

abx098858-200l 200 µl
EUR 1037.5

amfiRivert Platinum cDNA Synthesis Premix(2X)

R5700-096 96 rxns
EUR 562.8

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 861.6

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-100l 100 µl
EUR 1025

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-1ml 1 ml Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-200l 200 µl Ask for price

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
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First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

abx098016-100l 100 µl
EUR 775

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

abx098016-1ml 1 ml Ask for price

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

abx098016-200l 200 µl
EUR 912.5

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

abx098021-100l 100 µl
EUR 962.5

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

abx098021-1ml 1 ml Ask for price

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

abx098021-200l 200 µl
EUR 1237.5

amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-050 50 rxns
EUR 400.8

amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-100 2X50 rxns
EUR 643.2

amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-200 4x50 rxns
EUR 1048.8

amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-500 10X50 rxns
EUR 1842

One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction)

MB1003-100Reactions 100 Reactions
EUR 895.2

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
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First-Strand cDNA Synthesis SuperMix for PCR

abx098018-100l 100 µl
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First-Strand cDNA Synthesis SuperMix for PCR

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First-Strand cDNA Synthesis SuperMix for PCR

abx098018-200l 200 µl Ask for price

HyperScript First-Strand cDNA Synthesis SuperMix

K1073-100 100 rxn (20 uL/rxn)
EUR 282
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

HyperScript First-Strand cDNA Synthesis SuperMix

K1073-50 50 rxn (20 uL/rxn)
EUR 150
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-100l 100 µl
EUR 1012.5

Uni Cell to cDNA Synthesis SuperMix for qPCR

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Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-200l 200 µl Ask for price

EntiLink™ 1st Strand cDNA Synthesis Super Mix

EQ031 100 rxn
EUR 399

Accuris qMAX First Strand cDNA Synthesis Flex Kit, 200 reactions

PR2110-200 1 PC
EUR 435.18

Accuris qMAX First Strand cDNA Synthesis Flex Kit, 50 reactions

PR2110-50 1 PC
EUR 107.94

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 1020

The most common differential expression fracture and non-confused micro-micro-micro-micro-micro-micro-metal markers also have distinguished groups of patients with a different risk of metastasis, as well as the overall survival of the disease and the disease. In addition, the pathological fracture profile has demonstrated a higher differential expression for micro-equipped markers that were previously associated with a higher risk of metastases and higher survival rates in patients with osteosarcoma. patients with osteosarcoma, microad profiles thereof with pathological fractures are different. that patients without pathological fractures.