Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.

Vaccines with live Newcastle, low virulence (NDV) virus strains are still accepted prevention and control strategies for the fight against Newcastle disease (ND), a major viral disease hinders the development of the poultry industry around the world. However, the underlying mechanism for innate immune responses in the innate cell of the vaccine is unclear. Here, a broadband Illumona sequencing approach has been used to determine the expression profiles of cellular mina in lasota-infected chicken macrophages, a vaccine strain widely used for mass vaccination programs against nd in poultry.

Compared to the control group, 112 and 115 expressed differentially (de) Mirnas have been identified at 24 HPI (hours after inoculation) and 48 HPI, respectively. Meanwhile, 174 Mirnas were identified between 24 HPI and 48 HPI. In addition, 12 regulated and 6 MIRNAS were observed at 24 and 48 HPI compared to 0 HPI. In addition, the target prediction and functional analysis of these mirnas revealed a significant enrichment for several signaling pathways, particularly in genes and routes related to the immune, such as the signaling path of the RIG type receiver. -II-LIKE-I-like, a signaling channel of the Kinase Protein (MAPK) (MAPK) (MAPK) signaling receiver. Our conclusions not only have the foundations base for more research the roles and mechanisms of MIRNA regulatory in immunized immune responses mediated by vaccination, but also extend new perspectives in the interactions between the army and the NDV infection. To determine the microarna expression profile (MIRNA) in the mononuclear cells of the peripheral blood (PBMC) and immunized factors in pregnant women with hepatitis B virus infection (VHB).

A total of 182 pregnant women infected with HBV were randomly selected, 40 pregnant pregnant in good health women and 35 non-pregnant women as controls. High speed sequencing has been used to detect RNA in the PBMC of all subjects. The indirect ELISA method has been used to determine cytokine changes in peripheral blood samples. Comparked with the control group, 18 MARN expressed differentially have been identified in those with HBV infection (P <0, 01).

Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.
Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.

 

Clinical and molecular analysis of osteosarcoma associated with pathological fractures: the micro-portion profile is different and is correlated with prognosis.

The micrarnas are small non-coding RNAs that govern the expression of post-procedures genes. The presence of certain specific microarnas has been associated with an increased risk of local recurrence and metastasis and worse survival in patients with osteosarcoma. Pathological fractures in osteosarcoma are considered more the manifestation of a neoplasm with more aggressive biological behavior than the cause itself of the worst prognosis. However, this has not been proven at the biological or molecular level. Currently, there has been no microna profiling study of patients with osteosarcoma with and without pathological fractures that have described differences in microorna profiling between these two groups and their correlation with biological behavior.

(1) In patients with osteosarcoma extremities, how do the profiles of micro-portions thereof with and without pathological fractures do they compare? (2) What relations have microarnas with local recurrence, the risk of metastases, the survival specific to a disease and overall survival of patients with osteosarcoma with pathological fractures? Between 1994 and 2013, 217 patients were diagnosed and treated in our institution for osteosarcoma extremities. Patients were excluded if (1) they suffered oncolological resection of osteosarcoma in an external institution (two patients) or (2) they were diagnosed with an osteosarcoma extras (29 patients) or (3), They had less than a year of clinical follow-up -up and no oncolological result (local recurrence, metastasis or death) (four patients). In total, 182 patients were eligible. Of these 143 were high quality osteosarcomas. After the evaluation of tumor samples before the treatment of chemotherapy, a total of 80 consecutive samples were selected for sequencing. The demographic and clinical comparison between sequenced and non-sequenced patients has demonstrated no difference, confirming that both groups were comparable.

Diagnostic samples of the 80 patients with high-quality end osteosarcomas that had not yet received chemotherapy undergoes micro-microorna sequencing for a large-scale osteosarcoma genome profiling project in our institution. Six samples were removed after a second aspect by a musculoskeletal pathologist who verified the celluliarity and the quality of the samples to be sequenced, leaving a total of 74 patients. Of these, two samples have been removed because they have been confirmed to be pelvic tumors in a second check after sequencing. The final sample of the study was 72 patients (11 patients with pathological fractures and 61 without). The sequencing data has been correlated with local fractures and recurrence, a risk of metastases, survival specific to overall disease and survival through Kaplan-Meier.seral Micronas analyzes has been expressed differently between the two groups. Among the markers with the highest differential expression (Edger and DESEQ algorithms), and HSA-MIR 381-3P have been regulated in patients with pathological fractures, while that have been regulated.

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 309

cDNA Synthesis SuperMix

20-abx09801420ulSystems
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miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

MR101-01 50 rxn (20 μl/rxn)
EUR 198

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

MR101-02 100 rxn (20 μl/rxn)
EUR 285

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 224

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 544

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
EUR 337.75
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Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
EUR 142.36
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Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
EUR 711.77
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Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
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T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805
  • Category: Cas9

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 153

AzuraQuant cDNA Synthesis Kit - 100 Reactions

AZ-1996 100 Reactions
EUR 372

Optimax First Strand cDNA Synthesis Kit

K4201100 100 rnx
EUR 419

EpiNext Hi-Fi cDNA Synthesis Kit

P-9004 20 Samples
EUR 318.25
Description: Ask the seller for details

HiFiScript 1st Strand cDNA Synthesis Kit

W2569-10 NULL
EUR 0

HiFiScript 1st Strand cDNA Synthesis Kit

W2569-100 NULL
EUR 0

HiFiScript 1st Strand cDNA Synthesis Kit

W2569-30 NULL
EUR 0

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
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  • Category: Cas9

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
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amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 415

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 847

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1211

amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
EUR 134

amfiRivet cDNA Synthesis 2X Buffer

R5102-100 2x500ul
EUR 192

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1152
  • Category: Stem Cell Products

Second Strand cDNA Synthesis Kit-dNTP based

G475 25 x 50 ul reactions
EUR 389

HiScript II 1st Strand cDNA Synthesis Kit

R211-01 50 rxn (20 μl/rxn)
EUR 184

HiScript II 1st Strand cDNA Synthesis Kit

R211-02 100 rxn (20 μl/rxn)
EUR 251

RNA-Quant cDNA synthesis Kit (20 reactions)

RA430A-1 20 reactions
EUR 682
  • Category: MicroRNA Tools

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
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amfiRivert II cDNA Synthesis Master Mix

R5500-050 50 rxns
EUR 374

amfiRivert II cDNA Synthesis Master Mix

R5500-100 2x50rxns
EUR 577

amfiRivert cDNA Synthesis Prime Master Mix

R5610-050 50 rxns
EUR 412

amfiRivert cDNA Synthesis Prime Master Mix

R5610-100 2x50 rxns
EUR 671

amfiRivert cDNA Synthesis Prime Master Mix

R5610-200 4X50 rxns
EUR 1211

amfiRivert cDNA Synthesis Prime Master Mix

R5610-500 10x50 rxns
EUR 2548

amfiRivert Platinum cDNA Synthesis Premix(2X)

R5700-096 96 rxns
EUR 469

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-560 20 rcs
EUR 102

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-562 100 rcs
EUR 302

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-564 1000 rcs
EUR 2265

igScript First Strand cDNA Synthesis Kit - 25 Reactions

4312 1/EA
EUR 149

igScript First Strand cDNA Synthesis Kit - 100 Reactions

4314 1/EA
EUR 378

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based

G476 25 x 50 ul reactions
EUR 478

Accuris qMAX First Strand cDNA Synthesis Flex Kit

PR2110-200 1 PC
EUR 337.75
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Accuris qMAX First Strand cDNA Synthesis Flex Kit

PR2110-50 1 PC
EUR 132.79
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Accuris qMAX First Strand cDNA Synthesis Flex Kit

PR2110-S 1 PC
EUR 72.98
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First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
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First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 578
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cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 718
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amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-050 50 rxns
EUR 334

amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-100 2X50 rxns
EUR 536

amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-200 4x50 rxns
EUR 874

amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-500 10X50 rxns
EUR 1535

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R312-01 50 rxn (20 μl/rxn)
EUR 239

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R312-02 100 rxn (20 μl/rxn)
EUR 310

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • EUR 1024.00
  • EUR 746.00
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amfiRivert Platinum cDNA Synthesis Premix(2X), 480 tubes

R5700-480 5x96 rsnx
EUR 1927

amfiRivert Platinum ONE cDNA Synthesis Master Mix(4X)

R6100-050 50 rxns
EUR 300

amfiRivert Platinum ONE cDNA Synthesis Master Mix(4X)

R6100-100 2X50 rxns
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amfiRivert Platinum ONE cDNA Synthesis Master Mix(4X)

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EUR 779

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One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)

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One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction)

MB1003-100Reactions 100 Reactions
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One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

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One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)

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All-in-One First-Strand cDNA Synthesis SuperMix for PCR

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amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

R5600-050 50 rxns
EUR 235

amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

R5600-100 2X50 rxns
EUR 381

amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

R5600-200 4x50 rxns
EUR 658

amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

R5600-500 10X50 rxns
EUR 1333

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

OneScriptcDNA Synthesis Kit

G233 25 x 20 ul reactions
EUR 89

OneScriptcDNA Synthesis Kit

G234 100 x 20 ul reactions
EUR 141

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

CRISPR sgRNA Synthesis Kit

K1251-25
EUR 403

Evo? cDNA Kit

M1164-100
EUR 294

Evo? cDNA Kit

M1164-25
EUR 234

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

Classic CRISPR sgRNA Synthesis Kit

K1252-25 25 Reactions
EUR 439
  • Kit components:
  • 2X Classic sgRNA Synthesis SuperMix
  • Scaffold Template and Primer
  • sgRNA Control Oligo (10 ?M)
  • OneScribe Enzyme Mix
  • DNase I, RNase-Free (2 U/?l)
  • 10X DNase I Reaction Buffer
  • DistantTM PCR Mix (#M1136-200)
  • Nuclease-free H2O
Description: Classic CRISPR sgRNA Synthesis Kit

Express CRISPR sgRNA Synthesis Kit

K1253-25
EUR 533

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 89

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 141

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Novo? Transcriptome cDNA Kit

M1167-100
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25
EUR 441

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

EZClick? Global Protein Synthesis Assay Kit

K467-100
EUR 533

AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro]

GE602A-1 10 ug
EUR 1268
  • Category: Gene Editing

Glutamine Synthesis stable cells

SC013 2 x 106 cell/ml x 1ml
EUR 1210
Description: Stable cell line expressing Glutamine Synthesis gene and RFP with Blasticidin resistance

Evo? cDNA Kit (gDNA Removal)

M1166-100
EUR 381

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153
  • Category: Cas9

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Molybdenum Cofactor Synthesis 2 Protein

20-abx263321
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.

The most common differential expression fracture and non-confused micro-micro-micro-micro-micro-micro-metal markers also have distinguished groups of patients with a different risk of metastasis, as well as the overall survival of the disease and the disease. In addition, the pathological fracture profile has demonstrated a higher differential expression for micro-equipped markers that were previously associated with a higher risk of metastases and higher survival rates in patients with osteosarcoma. patients with osteosarcoma, microad profiles thereof with pathological fractures are different. that patients without pathological fractures.