Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.

Vaccines with live Newcastle, low virulence (NDV) virus strains are still accepted prevention and control strategies for the fight against Newcastle disease (ND), a major viral disease hinders the development of the poultry industry around the world. However, the underlying mechanism for innate immune responses in the innate cell of the vaccine is unclear. Here, a broadband Illumona sequencing approach has been used to determine the expression profiles of cellular mina in lasota-infected chicken macrophages, a vaccine strain widely used for mass vaccination programs against nd in poultry.

Compared to the control group, 112 and 115 expressed differentially (de) Mirnas have been identified at 24 HPI (hours after inoculation) and 48 HPI, respectively. Meanwhile, 174 Mirnas were identified between 24 HPI and 48 HPI. In addition, 12 regulated and 6 MIRNAS were observed at 24 and 48 HPI compared to 0 HPI. In addition, the target prediction and functional analysis of these mirnas revealed a significant enrichment for several signaling pathways, particularly in genes and routes related to the immune, such as the signaling path of the RIG type receiver. -II-LIKE-I-like, a signaling channel of the Kinase Protein (MAPK) (MAPK) (MAPK) signaling receiver. Our conclusions not only have the foundations base for more research the roles and mechanisms of MIRNA regulatory in immunized immune responses mediated by vaccination, but also extend new perspectives in the interactions between the army and the NDV infection. To determine the microarna expression profile (MIRNA) in the mononuclear cells of the peripheral blood (PBMC) and immunized factors in pregnant women with hepatitis B virus infection (VHB).

A total of 182 pregnant women infected with HBV were randomly selected, 40 pregnant pregnant in good health women and 35 non-pregnant women as controls. High speed sequencing has been used to detect RNA in the PBMC of all subjects. The indirect ELISA method has been used to determine cytokine changes in peripheral blood samples. Comparked with the control group, 18 MARN expressed differentially have been identified in those with HBV infection (P <0, 01).

Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.
Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota.

 

Clinical and molecular analysis of osteosarcoma associated with pathological fractures: the micro-portion profile is different and is correlated with prognosis.

The micrarnas are small non-coding RNAs that govern the expression of post-procedures genes. The presence of certain specific microarnas has been associated with an increased risk of local recurrence and metastasis and worse survival in patients with osteosarcoma. Pathological fractures in osteosarcoma are considered more the manifestation of a neoplasm with more aggressive biological behavior than the cause itself of the worst prognosis. However, this has not been proven at the biological or molecular level. Currently, there has been no microna profiling study of patients with osteosarcoma with and without pathological fractures that have described differences in microorna profiling between these two groups and their correlation with biological behavior.

(1) In patients with osteosarcoma extremities, how do the profiles of micro-portions thereof with and without pathological fractures do they compare? (2) What relations have microarnas with local recurrence, the risk of metastases, the survival specific to a disease and overall survival of patients with osteosarcoma with pathological fractures? Between 1994 and 2013, 217 patients were diagnosed and treated in our institution for osteosarcoma extremities. Patients were excluded if (1) they suffered oncolological resection of osteosarcoma in an external institution (two patients) or (2) they were diagnosed with an osteosarcoma extras (29 patients) or (3), They had less than a year of clinical follow-up -up and no oncolological result (local recurrence, metastasis or death) (four patients). In total, 182 patients were eligible. Of these 143 were high quality osteosarcomas. After the evaluation of tumor samples before the treatment of chemotherapy, a total of 80 consecutive samples were selected for sequencing. The demographic and clinical comparison between sequenced and non-sequenced patients has demonstrated no difference, confirming that both groups were comparable.

Diagnostic samples of the 80 patients with high-quality end osteosarcomas that had not yet received chemotherapy undergoes micro-microorna sequencing for a large-scale osteosarcoma genome profiling project in our institution. Six samples were removed after a second aspect by a musculoskeletal pathologist who verified the celluliarity and the quality of the samples to be sequenced, leaving a total of 74 patients. Of these, two samples have been removed because they have been confirmed to be pelvic tumors in a second check after sequencing. The final sample of the study was 72 patients (11 patients with pathological fractures and 61 without). The sequencing data has been correlated with local fractures and recurrence, a risk of metastases, survival specific to overall disease and survival through Kaplan-Meier.seral Micronas analyzes has been expressed differently between the two groups. Among the markers with the highest differential expression (Edger and DESEQ algorithms), and HSA-MIR 381-3P have been regulated in patients with pathological fractures, while that have been regulated.

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

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OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
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OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 202.8

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
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EVery cDNA Synthesis Kit

EVery200B-1 20 preps
EUR 307

cDNA Synthesis SuperMix

20-abx09801420ulSystems
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miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

MR101-01 50 rxn (20 μl/rxn)
EUR 237.6

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

MR101-02 100 rxn (20 μl/rxn)
EUR 342

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
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Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
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Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
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Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
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Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
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Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
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T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
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OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
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OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
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AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 183.6

AzuraQuant cDNA Synthesis Kit - 100 Reactions

AZ-1996 100 Reactions
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Optimax First Strand cDNA Synthesis Kit

K4201100 100 rnx
EUR 502.8

EpiNext Hi-Fi cDNA Synthesis Kit  

P-9004-20 20 Reactions
EUR 227.7

EpiNext Hi-Fi cDNA Synthesis Kit  

P-9004
  • EUR 381.90
  • EUR 227.70
  • 20 Samples
  • 20 Reactions

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
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amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
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amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
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amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
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amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
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amfiRivet cDNA Synthesis 2X Buffer

R5102-100 2x500ul
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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS410A-KIT 1 kit (10 rxn)
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mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1382.4

Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS420A-KIT 1 kit (10 rxn)
EUR 828

Second Strand cDNA Synthesis Kit-dNTP based

G475 25 x 50 ul reactions
EUR 466.8

HiScript II 1st Strand cDNA Synthesis Kit

R211-01 50 rxn (20 μl/rxn)
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HiScript II 1st Strand cDNA Synthesis Kit

R211-02 100 rxn (20 μl/rxn)
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RNA-Quant cDNA synthesis Kit (20 reactions)

RA430A-1 20 reactions
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Fly First-Strand cDNA Synthesis SuperMix

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All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

amfiRivert II cDNA Synthesis Master Mix

R5500-050 50 rxns
EUR 448.8

amfiRivert II cDNA Synthesis Master Mix

R5500-100 2x50rxns
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amfiRivert cDNA Synthesis Prime Master Mix

R5610-050 50 rxns
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amfiRivert cDNA Synthesis Prime Master Mix

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amfiRivert cDNA Synthesis Prime Master Mix

R5610-200 4X50 rxns
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amfiRivert cDNA Synthesis Prime Master Mix

R5610-500 10x50 rxns
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amfiRivert Platinum cDNA Synthesis Premix(2X)

R5700-096 96 rxns
EUR 562.8

igScript First Strand cDNA Synthesis Kit - 25 Reactions

4312 1/EA
EUR 178.8

igScript First Strand cDNA Synthesis Kit - 100 Reactions

4314 1/EA
EUR 453.6

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-560 20 rcs
EUR 122.4

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-562 100 rcs
EUR 362.4

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-564 1000 rcs
EUR 2718

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based

G476 25 x 50 ul reactions
EUR 573.6

Accuris qMAX First Strand cDNA Synthesis Flex Kit

PR2110-200 1 PC
EUR 405.3

Accuris qMAX First Strand cDNA Synthesis Flex Kit

PR2110-50 1 PC
EUR 159.35

Accuris qMAX First Strand cDNA Synthesis Flex Kit

PR2110-S 1 PC
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First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

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First-Strand cDNA Synthesis SuperMix for PCR

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cDNA Synthesis SuperMix for qPCR (GC-rich)

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amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-050 50 rxns
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amfiRivert Sensi cDNA Synthesis Master Mix(4X)

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amfiRivert Sensi cDNA Synthesis Master Mix(4X)

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amfiRivert Sensi cDNA Synthesis Master Mix(4X)

R6200-500 10X50 rxns
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HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R312-01 50 rxn (20 μl/rxn)
EUR 286.8

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

R312-02 100 rxn (20 μl/rxn)
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One-Step gDNA Removal and cDNA Synthesis SuperMix

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  • EUR 895.20
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amfiRivert Platinum cDNA Synthesis Premix(2X), 480 tubes

R5700-480 5x96 rsnx
EUR 2312.4

amfiRivert Platinum ONE cDNA Synthesis Master Mix(4X)

R6100-050 50 rxns
EUR 360

amfiRivert Platinum ONE cDNA Synthesis Master Mix(4X)

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amfiRivert Platinum ONE cDNA Synthesis Master Mix(4X)

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One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction)

MB1003-100Reactions 100 Reactions
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One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

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One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)

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All-in-One First-Strand cDNA Synthesis SuperMix for PCR

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amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

R5600-050 50 rxns
EUR 282

amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

R5600-100 2X50 rxns
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amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

R5600-200 4x50 rxns
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amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer

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LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
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OneScriptcDNA Synthesis Kit

G233 25 x 20 ul reactions
EUR 106.8

OneScriptcDNA Synthesis Kit

G234 100 x 20 ul reactions
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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
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CRISPR sgRNA Synthesis Kit

K1251-25 each
EUR 483.6

Novo? cDNA Kit

M1165-100 each
EUR 424.8

Novo? cDNA Kit

M1165-25 each
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LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

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K1252-25 25 Reactions
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Description: Classic CRISPR sgRNA Synthesis Kit

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K1253-25 each
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OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
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OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
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Evo™ cDNA Kit

M1164-100 each Ask for price

Evo™ cDNA Kit

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Novo? Transcriptome cDNA Kit

M1167-100 each
EUR 1142.4

Novo? Transcriptome cDNA Kit

M1167-25 each
EUR 529.2

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro]

GE602A-1 10 ug
EUR 1521.6

PCR Mycoplasma Detection Kit

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EUR 319.2

EZClick? Global Protein Synthesis Assay Kit

K467-100 each
EUR 639.6

Glutamine Synthesis stable cells

SC013 2 x 106 cell/ml x 1ml
EUR 1452
Description: Stable cell line expressing Glutamine Synthesis gene and RFP with Blasticidin resistance

Evo? cDNA Kit (gDNA Removal)

M1166-100 each
EUR 457.2

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Evo? cDNA Supermix

M1168-100 each
EUR 457.2

Evo? cDNA Supermix

M1168-25 each
EUR 320.4

Novo? cDNA Supermix

M1169-100 each
EUR 529.2

Novo? cDNA Supermix

M1169-25 each
EUR 346.8

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1358.4

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CASLV205PA-KIT 1 Kit
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CASLV225PA-KIT 1 Kit
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Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
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Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
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Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
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Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
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Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
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Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

The most common differential expression fracture and non-confused micro-micro-micro-micro-micro-micro-metal markers also have distinguished groups of patients with a different risk of metastasis, as well as the overall survival of the disease and the disease. In addition, the pathological fracture profile has demonstrated a higher differential expression for micro-equipped markers that were previously associated with a higher risk of metastases and higher survival rates in patients with osteosarcoma. patients with osteosarcoma, microad profiles thereof with pathological fractures are different. that patients without pathological fractures.