MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen

Gestational diabetes mellitus (GDM), one of the complications associated with pregnancy is a big, characterized as a temporary form of diabetes caused by insulin resistance along with low / absent-adaptation pancreatic beta cells compensate for insulin demand increases, causing acute, long a term, and transgenerational health complications. The purpose of this study was to assess whether changes in gene expression of microRNAs associated with diabetes disease / cardiovascular / cerebrovascular present throughout the peripheral blood of children aged 3-11 years dropped from GDM complicated pregnancy.

A microRNA expression profiles to be substantially modified is found in children dropped from GDM pregnancies complicated. Almost all of microRNAs with the exception of miR-92A-3p, miR-155-5p, and miR-210-3p regulated. The microRNA expression profiles also differ between children after normal and GDM pregnancies complicated in relation to the presence of overweight / obesity, prehypertension / hypertension and / or heart valve problems and defects.

Always, screening by a combination of superior use microRNAs microRNAs individual, because the false positive rate of 10.0% was able to identify most children with aberrant expression of microRNA profiles (88.14% regardless of the clinical findings, 75.41% by clinically normal findings, and 96.49% with abnormal clinical findings). In addition, a higher incidence of heart valve problems and defects found in children with exposure before GDM.

Extensive file of the target prediction of all microRNAs aberrantly expressed in children dropped from GDM pregnancies complicated show that a large group of these genes are involved in ontology diabetes / cardiovascular / cerebrovascular disease. In general, children with exposure before GDM are at a higher risk of subsequent development of diabetes mellitus and cardiovascular / cerebrovascular disease, and would benefit from dispensarisation and implementation of primary prevention strategies.

 MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen
MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen

MicroRNA expression profiles in extracellular vesicles derived from ALV-J semen of infected chickens

MicroRNAs (miRNAs) have been reported to regulate the expression of genes in many processes. MiRNA in extracellular vesicles (EV) has also been studied, while no study miRNAs in EVS semen. Subgroup J Avian leukosis virus (ALV-J) can be transmitted vertically, but the mechanism was not clear enough. This study was to examine the expression profile of miRNA in EVs semen and to investigate the relationship between it and the vertical transmission by gene ontology (GO) and pathway enrichment analysis.

Here, we first isolated and characterized EVS semen with Nanoparticle Tracking Analysis, Western Blot and transmission electron microscopy experiments. By sequencing in each EVS miRNA library, a typical 9 differentially expressed miRNA, including six up-regulated and 3 down-regulated, are identified. Target gene prediction, explanation GO and KEGG pathway enrichment analysis shows that the function may be associated with these miRNAs. Overall, these findings will improve our understanding of the content and composition of the miRNA in EVs semen and provide new insights into the critical role of regulation of miRNAs EVS semen in the transmission ALV-J.

ApexPrep DNA Plasmid Miniprep Column Only

A5002-50 50 tests
EUR 87

EZ-10 Spin Column Total RNA Miniprep Kit

BS1361(SK8655) 50Preps
EUR 94.15
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

EZ-10 Spin Column DNA Cleanup Miniprep Kit

BS367 50Preps
EUR 76.64
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EZ-10 Spin Column DNA Cleanup Miniprep Kit

BS368 100preps
EUR 95.68
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EZ-10 Spin Column Plasmid DNA Miniprep Kit

BS413 50Preps
EUR 76.64
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EZ-10 Spin Column Plasmid DNA Miniprep Kit

BS414 100preps
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EZ-10 Spin Column DNA Cleanup Miniprep Kit

BS668 250preps
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EZ-10 Spin Column Blood RNA Miniprep Kit

BS82313 50Preps
EUR 107.09
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EZ-10 Spin Column Plant RNA Miniprep Kit

BS82314 50Preps
EUR 107.09
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BS91915 50Preps
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EZ-10 Spin Column Soil DNA Miniprep Kit

ST82316 50Preps
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EZ-10 Spin Column Viral DNA Miniprep Kit

VT81812 50Preps
EUR 224
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EZ-10 Spin Column Viral DNA Miniprep Kit

VT81813 250preps
EUR 843
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EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS423 50Preps
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BS427 50Preps
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BS467 10Preps
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EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit

BS468 50Preps
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BS469 250preps
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EZ-10 Spin Column Total RNA Miniprep Super Kit

BS583 20Preps
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BS584 100preps
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EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS624 250preps
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EZ-10 Spin Column Animal Genomic DNA Miniprep Kit

BS628 250preps
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EZ-10 Spin Column Total RNA Miniprep Super Kit

BS784 250preps
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EZ-10 Spin Column Animal Total RNA Miniprep Kit

BS82312 50Preps
EUR 97.2
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EZ-10 Spin Column Animal Total RNA Miniprep Kit

BS82322 250preps
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EZ-10 Spin Column Yeast Genomic DNA Miniprep Kit

BS8257 50Preps
EUR 94.15
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EZ-10 Spin Column Fungal Genomic DNA Miniprep Kit

FT82012 50Preps
EUR 81.97
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EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8253 50Preps
EUR 83.4
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EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8254 100preps
EUR 120.72
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EZ-10 Spin Column Endotoxin-Free Plasmid DNA Miniprep Kit

BS71918 50Preps
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EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid

BS4139 50Preps
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EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid

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Plasmid MiniPrep Kit

20-abx098081
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Plasmid Miniprep Kit

K529-100
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20-abx098082
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K1312-250
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K1312-50
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K1313-250
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K1313-50
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ViraBind Adenovirus Miniprep Kit

VPK-099 10 preps
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Pure Plasmid Miniprep Kit

W0500-200 NULL
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Zenoquick RNA Miniprep Kit

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EUR 286

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EUR 847

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9K-006-0009 100preps
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6554-1
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Frit Kit

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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

His-tag Protein Purification Mini Kit unit: 3-10mg/ column, 1 column

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21583 50 preps
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Prolytic His-Tagged Protein Miniprep Kit

P6400-010 10 prep
EUR 253

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P6400-050 50 Prep
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BS88133 50Preps
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K5011350 1 kit
EUR 237

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Z1007-100 2x50 preps
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6508-1
EUR 207

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6508-5
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6518-1
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6518-5
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6538-1
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One-4-All Genomic DNA Miniprep Kit

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BS88505 250preps
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96-Well Plate Viral DNA Miniprep Kit

VT92032 2XPlates, 192prep
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CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

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  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
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  • Category: Cas9

Spin Column for Zenoquick Viral RNA Kit

Z5014-C01 250/pk
EUR 435

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
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  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

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CASLV205PA-KIT 1 Kit
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Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
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  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
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  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
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  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
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  • Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
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  • Category: Cas9

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
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PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

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  • Category: PinPoint Integrase Tools

HisXL-Column High Density Cobalt

6BCL-QHCo-C5 5 x 5 ml
EUR 322

His-Column High Density Cobalt

6BCL-QHCo-C8 8 x 1 ml
EUR 148

HisXL-Column High Density Nickel

6BCL-QHNi-C5 5 x 5 ml
EUR 322

His-Column High Density Nickel

6BCL-QHNi-C8 8 x 1 ml
EUR 148

Protein A/G Sepharose Column

6528-1
EUR 327

Protein A/G Sepharose Column

6528-5
EUR 805

ToxOut? Protein A (Sepharose) Column

M2503-1
EUR 294

ToxOut? Protein A (Sepharose) Column

M2503-5
EUR 974

ToxOut? Protein G (Sepharose) Column

M4103-1
EUR 294

ToxOut? Protein G (Sepharose) Column

M4103-5
EUR 974

5? Polypropylene Disposable Gravity Column

M1314-50
EUR 164

7? Polypropylene Disposable Gravity Column

M1315-50
EUR 164

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-100Reactions 100 Reactions
EUR 143

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-250Reactions 250 Reactions
EUR 259

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-50Reactions 50 Reactions
EUR 120

96-Well Plate Animal Genomic DNA Miniprep Kit

BS437 5XPlates, 480prep
EUR 362.98
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96-Well Plate Animal Genomic DNA Miniprep Kit

BS4372 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96 Well Plate Yeast Genomic DNA Miniprep Kit

BS8357 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

96-Well Plate Plant Genomic DNA Miniprep Kit

BS8361 2XPlates, 192prep
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96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 2Plates, 192prep
EUR 175.71
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96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 5Plates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1292 2XPlates, 192prep
EUR 175.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1295 5XPlates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

FavorFilter Plasmid Maxi SP Kit (10prep)-spin column

FAPDE-005-010 10 preps
EUR 140

FavorFilter Plasmid Maxi SP Kit (20prep)-spin column

FAPDE-005-020 20 preps
EUR 169

FavorFilter Plasmid Midi SP Kit (25prep)-spin column

FAPDE-005-M-025 25 preps
EUR 150

FavorFilter Plasmid Midi SP Kit (50prep)-spin column

FAPDE-005-M-050 50 preps
EUR 187

EZ-10 Spin Column DNA Gel Extraction Kit

BS353 50Preps
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EZ-10 Spin Column DNA Gel Extraction Kit

BS354 100preps
EUR 95.68
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EZ-10 Spin Column PCR Products Purification Kit

BS363 50Preps
EUR 76.64
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EZ-10 Spin Column PCR Products Purification Kit

BS364 100preps
EUR 95.68
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EZ-10 Spin Column DNA Gel Extraction Kit

BS654 250preps
EUR 166.58
  • Product category: Molecular Biology Kits/DNA - Extraction/Gel Extraction

EZ-10 Spin Column PCR Products Purification Kit

BS664 250preps
EUR 166.58
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AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

Protein A/G/L Sepharose Column

6548-1
EUR 327


microRNAs (miRNAs) are small RNAs that regulate different biological processes. Our goal is to identify dysregulated miRNAs in the plasma and tissues of patients with abdominal aortic aneurysm (AAA) and exploring new potential targets involved in the AAA. Fifty-seven subjects were recruited for the study of plasma (30 patients with AAA, 16 healthy volunteers and 11 patients with atherosclerosis).