Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
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Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
intracranial aneurysm (IA) is a type of cerebrovascular disorders, which can lead to deadly subarachnoid hemorrhage with high handicaps. The purpose of this study was to reveal the pathogenesis and identify novel biomarkers in crude processed IA.We microRNA (miRNA) expression profiling data IA obtained from Gene Expression Omnibus.
Then the weighted correlation network analysis is conducted to identify miRNAs in IA hub. miRNAs target gene hub suspected of using multiR package. In addition, the network of proteins and miRNA-mRNA networks are built and functional and pathway enrichment analysis was performed. Finally, the predictive value of miRNAs in IA tested hubs in set.Two validation module that has a relationship with IA identified 10 miRNAs hub in each module with a gene associated higher-module selected. Network proteins and miRNA-mRNA networks that are 243 nodes and 1496 edges.
Functional and pathway enrichment analysis shows that they are especially enriched in cell cycle, cell proliferation, and PI3K / Akt signaling pathway. Additionally, HSA-miR-191-3p, HSA-miR-423-5p, HSA-miR-424-5p, HSA-miR-425-3p that proved valuable in the prediction IA occurrence.In words, the study revealed hub miRNAs , target genes and pathways potentially participate in the formation and development of IA and screen several biomarker candidates. Our findings provide some new perspectives for research and treatment of IA.
Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
MicroRNAs Exosomal expression profile of HEV- and HCV-Infected Blood Donors and Patients: Pilot Study
Exosomes appears to play an important role in hepatitis C virus (HCV) and hepatitis E virus (HEV) infection to protect their cargo from the host immune response, with microRNAs become a key component exosomal. Little is known about their involvement in HCV infection mix / HEV or in the early stages of infection, such as in asymptomatic blood donors (BD). To obtain preliminary data, we have compared the microRNA expression profiles in four exosomal each HCV RNA-positive blood donors HEV RNA positive and negative and four patients, one of which is a rare patients with HCV / HEV co-infection.
Exosomes purified from sera by a combination of precipitation and density gradient centrifugation and exosomal microRNA analyzed using the TaqMan array cards. Of the 33 deregulated miRNAs, miR-885-5p and miR-365 regulated in HCV BD, miR-627-5p was downregulated in HCV BD and miR-221 were downregulated in patients with HCV and BD. In HEV infection, miR-526B appears specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) shows potential involvement in both infections. First preliminary data on the treatment of exosomal microRNA profiles before and after antiviral of patients HEV / HCV co-infected reveal puddle 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential role in the infection of this virus.
This is a case-control study including CSF specimens from 41 patients with children. CSF specimens categorized into FS and control groups. microarray tests were conducted to evaluate CSF exosomal miRNA expression profiles. Quantitative PCR (qPCR) assays were performed to validate the microarray assay results. bioinformatics analysis was conducted to analyze the results. Thirteen (62%) patients in group FS experienced a complex FS.
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)
Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)
Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
Description: microRNA-21-IN-2 is a potential miR-21 inhibitor with an AC50 value of 3.29 μM. microRNA-21-IN-2 can be used for the research of cancer[1].
Description: microRNA-21-IN-3 (compound 45) can specifically bind to the precursor of oncogenic and pro-inflammatory microRNA-21 with medium nanomolar affinity, reduce cancer cell proliferation and miR-21 levels, and can be used in antitumor research[1].
Description: microRNA-21-IN-1 (compound 7A) is an efficient microRNA inhibitor. microRNA-21-IN-1 has antiproliferative activity against Hela and HCT-116 cells with IC50s of 5.5 μM and 2.8 μM respectively, as well as promotes apoptosis of Hela cells. microRNA-21-IN-1 upregulates the expression of microRNA-21 downstream functional targets (PTEN, EGR1 and SLIT2). microRNA-21-IN-1 can be used for researching anticancer[1].
A total of 96 miRNAs significantly expressed in the study sample CSF and 95 between them, exhibited a higher expression in the FS than the control group. Further validation tests qPCR showed that the top 5 highly expressed miRNA (miR-4486, mir-6850-5p, miR-642b-3p, miR-7107-5p, mir-4281) showed the same results as in the microarray test. bioinformatics analysis identified 455 target genes in the FS group.
