Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
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Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
intracranial aneurysm (IA) is a type of cerebrovascular disorders, which can lead to deadly subarachnoid hemorrhage with high handicaps. The purpose of this study was to reveal the pathogenesis and identify novel biomarkers in crude processed IA.We microRNA (miRNA) expression profiling data IA obtained from Gene Expression Omnibus.
Then the weighted correlation network analysis is conducted to identify miRNAs in IA hub. miRNAs target gene hub suspected of using multiR package. In addition, the network of proteins and miRNA-mRNA networks are built and functional and pathway enrichment analysis was performed. Finally, the predictive value of miRNAs in IA tested hubs in set.Two validation module that has a relationship with IA identified 10 miRNAs hub in each module with a gene associated higher-module selected. Network proteins and miRNA-mRNA networks that are 243 nodes and 1496 edges.
Functional and pathway enrichment analysis shows that they are especially enriched in cell cycle, cell proliferation, and PI3K / Akt signaling pathway. Additionally, HSA-miR-191-3p, HSA-miR-423-5p, HSA-miR-424-5p, HSA-miR-425-3p that proved valuable in the prediction IA occurrence.In words, the study revealed hub miRNAs , target genes and pathways potentially participate in the formation and development of IA and screen several biomarker candidates. Our findings provide some new perspectives for research and treatment of IA.
Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
MicroRNAs Exosomal expression profile of HEV- and HCV-Infected Blood Donors and Patients: Pilot Study
Exosomes appears to play an important role in hepatitis C virus (HCV) and hepatitis E virus (HEV) infection to protect their cargo from the host immune response, with microRNAs become a key component exosomal. Little is known about their involvement in HCV infection mix / HEV or in the early stages of infection, such as in asymptomatic blood donors (BD). To obtain preliminary data, we have compared the microRNA expression profiles in four exosomal each HCV RNA-positive blood donors HEV RNA positive and negative and four patients, one of which is a rare patients with HCV / HEV co-infection.
Exosomes purified from sera by a combination of precipitation and density gradient centrifugation and exosomal microRNA analyzed using the TaqMan array cards. Of the 33 deregulated miRNAs, miR-885-5p and miR-365 regulated in HCV BD, miR-627-5p was downregulated in HCV BD and miR-221 were downregulated in patients with HCV and BD. In HEV infection, miR-526B appears specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) shows potential involvement in both infections. First preliminary data on the treatment of exosomal microRNA profiles before and after antiviral of patients HEV / HCV co-infected reveal puddle 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential role in the infection of this virus.
This is a case-control study including CSF specimens from 41 patients with children. CSF specimens categorized into FS and control groups. microarray tests were conducted to evaluate CSF exosomal miRNA expression profiles. Quantitative PCR (qPCR) assays were performed to validate the microarray assay results. bioinformatics analysis was conducted to analyze the results. Thirteen (62%) patients in group FS experienced a complex FS.
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components)
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.
To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
Biolipidure does not require biohazardous handling.
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement
30UL FINE POINT MAXYMUM RECOVERY TIP. 384 TIPS PER RACK
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)
Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)
A total of 96 miRNAs significantly expressed in the study sample CSF and 95 between them, exhibited a higher expression in the FS than the control group. Further validation tests qPCR showed that the top 5 highly expressed miRNA (miR-4486, mir-6850-5p, miR-642b-3p, miR-7107-5p, mir-4281) showed the same results as in the microarray test. bioinformatics analysis identified 455 target genes in the FS group.
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Mouse SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set (20 assays) 
Regulatory RNA qPCR Profiler 
SeraMir Control spike-in Small RNA and qPCR assay 
SeraMir Exosome RNA Ampflication Kit 
Neural Lineage qPCR Profiler )
EZScreen? Glucose Colorimetric Assay Kit (384 Well) )
EZScreen? Lactate Colorimetric Assay Kit (384 Well) )
SeraMir Exosome RNA Purification Column kit (20 exoRNA columns) 
Mouse pre-microRNA Expression Construct mir-384 )
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns) )
SeraMir 3'-Universal Primer (10,000 qPCR reactions) 
Cancer MicroRNA qPCR Array 
hCRP FRET 384 
hCRP TRF 384 
hAlbumin FRET 384 
hAlbumin TRF 384 
hIgG TRF 384 
hInsulin FRET 384 
hInsulin TRF 384 
mIgM TRF 384 
mIgG TRF 384 
rIgG FRET 384 
Mouse IgM FRET 384 
Mouse IgG FRET 384  20 profiles)
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles 
Psoriasis Exosome RNA 
Opti-Seal qPCR 384 Well Plate 
Bridge-It cAMP-PDE 384 
Biotin TRF 384  20 profiles)
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles  10 preps)
SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps 
Normal human exosome RNA 
Leukemia - ALL Exosome RNA 
Leukemia - AML Exosome RNA 
Bladder Cancer Exosome RNA 
Breast Cancer Exosome RNA 
Leukemia - CLL Exosome RNA 
Leukemia - CML Exosome RNA 
Colorectal Cancer Exosome RNA 
Crohn's Disease Exosome RNA 
Kidney Cancer Exosome RNA 
Lung Cancer Exosome RNA 
Liver Cancer Exosome RNA 
Nasopharyngeal Cancer Exosome RNA 
Prostate Cancer Exosome RNA 
Rheumatoid Arthritis Exosome RNA 
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PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 
384 WELL PCR MICROPLATE. CLEAR 
384 Well Plate, 10/Bag )
SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns) )
Total Cholesterol Assay Kit (Colorimetric)  20 profiles)
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles 
Green RNA Lysis Kit 50 
Green RNA Lysis Kit 250 
Navy RNA Lysis Kit 50 
Navy RNA Lysis Kit 250 
Pink RNA Lysis Kit 50 
Pink RNA Lysis Kit 250 
Red RNA Lysis Kit 50 
Red RNA Lysis Kit 250 
YELLOW RNA Lysis Kit 50 
YELLOW RNA Lysis Kit 250 
LiqBiopsy Tumor Exosome RNA Kit  Exosome RNA)
Diabetes (Type 1) Exosome RNA  Exosome RNA)
Diabetes (Type 2) Exosome RNA 
Leukemia - Hairy Cell Exosome RNA 
Leukemia - Multiple Myeloma Exosome RNA  Exosome RNA)
Melanoma (skin cancer) Exosome RNA 
Non-Hodgkin lymphoma Exosome RNA 
Systemic Lupus Erythematosus Exosome RNA 
Uterine Cervical Cancer Exosome RNA 
Uterine Corpus Cancer Exosome RNA 
Radius 384-Well Cell Migration Assay )
Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS) 
5E GREEN RNA Lysis Kit 100 
5E GREEN RNA Lysis Kit 20 
Green RINO RNA Lysis Kit 50 
Green RINO RNA Lysis Kit 250 
5E Navy RNA Lysis Kit 100 
5E Navy RNA Lysis Kit 20 
Navy RINO RNA Lysis Kit 50 
Navy RINO RNA Lysis Kit 250 
5E PINK RNA Lysis Kit 100 
5E PINK RNA Lysis Kit 20 
Pink RINO RNA Lysis Kit 50 
Pink RINO RNA Lysis Kit 250 
5E RED RNA Lysis Kit 100 
5E RED RNA Lysis Kit 20 
Red RINO RNA Lysis Kit 50 
Red RINO RNA Lysis Kit 250 
YELLOW RINO RNA Lysis Kit 50 
YELLOW RINO RNA Lysis Kit 250 )
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components) )
Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA812A-1 components) 
50UL 384 WELL PIPET TIPS FOR FINNPIPETTE PIPETTORS 
CytoSelect 384-well Cell Transformation Assay, Fluorometric )
EZScreen? ATP Colorimetric Assay Kit (384-well) )
EZScreen? Glycogen Colorimetric Assay Kit (384-Well)  Antibody)
Exosome RNA Helicase MTR4 (MTR4) Antibody 
ExoQuick Exosome RNA Column Purification kit 
Human pre-microRNA Expression Construct Lenti-miR-384 
Human CD9 Expressed Exosome Detection Assay kit 
Human CD63 Expressed Exosome Detection Assay Kit )
TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay) )
TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay) )
TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay) )
NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay) )
NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay) )
NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay) )
GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay) )
GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay) )
GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay) 
384 WELL BLACK RIGI PLATE PCR MICROPLATE  20 profiles)
Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA811A-1 components) 20 profiles 
Equalbit RNA HS Assay Kit 
Equalbit RNA BR Assay Kit )
EZScreen? Triglyceride Quantification Colorimetric Assay Kit (384-well) )
EZScreen? NAD+/NADH Colorimetric Assay Kit (384-well)  Antibody)
Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody 
384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR )
ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay) )
ExoPure? Immunoplates (Overall Exosome Isolation, serum, luminometric assay) )
ExoPure? Immunoplates (Overall Exosome Isolation, serum, fluorimetric assay) )
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay) )
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay) )
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay) )
Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay) )
Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay) )
Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay) 
TC 9639 Gradient Thermal Cycler Touchscreen with 384 well block with EU Plug )
EZScreen? Lactate Dehydrogenase Activity Colorimetric Assay Kit (384-well) )
EZScreen? Beta-Lactamase Activity Colorimetric Assay Kit (384-well) )
EZScreen? Free Fatty Acid Colorimetric Assay Kit (384-well) 
384 WELL CLEAR 30 µLTIPS FOR MOLECULAR DEVICE FLIPR TETRA. 384 TIPS PER RACK, 10 RACKS PER UNIT, 5 UNITS PER CASE )
ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay) )
ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, luminometric assay) )
ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, fluorometric assay) 
384 WELL BLACK 30 µL TIPS FOR MOLECULAR DEVICE FLIPR TETRA. 384 TIPS PER RACK, 10 RACKS PER UNIT, 5 UNITS PER CASE 
SEALING MAT, FOR 384 WELL PCR MICROPLATES, SILICONE, NATURAL, NONSTERILE, BULK 
10UL MAXYMUM RECOVERY TIP, CLEAR. 384 TIPS PER RACK 
5UL MAXYMUM RECOVERY TIP, CLEAR. 384 TIPS PER RACK 
70UL MAXYMUM RECOVERY TIP, CLEAR. 384 TIPS PER RACK )
EZScreen? Total Cholesterol & Cholesteryl Ester Colorimetric Assay Kit (384-Well) 
Set of 10 Biolipidure Reagents 
30UL FINE POINT MAXYMUM RECOVERY TIP. 384 TIPS PER RACK , Sterile-Filtered)
StemBoost? YPAC Cocktail Set (1000X), Sterile-Filtered )
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components) )
Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)