Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
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Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
intracranial aneurysm (IA) is a type of cerebrovascular disorders, which can lead to deadly subarachnoid hemorrhage with high handicaps. The purpose of this study was to reveal the pathogenesis and identify novel biomarkers in crude processed IA.We microRNA (miRNA) expression profiling data IA obtained from Gene Expression Omnibus.
Then the weighted correlation network analysis is conducted to identify miRNAs in IA hub. miRNAs target gene hub suspected of using multiR package. In addition, the network of proteins and miRNA-mRNA networks are built and functional and pathway enrichment analysis was performed. Finally, the predictive value of miRNAs in IA tested hubs in set.Two validation module that has a relationship with IA identified 10 miRNAs hub in each module with a gene associated higher-module selected. Network proteins and miRNA-mRNA networks that are 243 nodes and 1496 edges.
Functional and pathway enrichment analysis shows that they are especially enriched in cell cycle, cell proliferation, and PI3K / Akt signaling pathway. Additionally, HSA-miR-191-3p, HSA-miR-423-5p, HSA-miR-424-5p, HSA-miR-425-3p that proved valuable in the prediction IA occurrence.In words, the study revealed hub miRNAs , target genes and pathways potentially participate in the formation and development of IA and screen several biomarker candidates. Our findings provide some new perspectives for research and treatment of IA.
Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
MicroRNAs Exosomal expression profile of HEV- and HCV-Infected Blood Donors and Patients: Pilot Study
Exosomes appears to play an important role in hepatitis C virus (HCV) and hepatitis E virus (HEV) infection to protect their cargo from the host immune response, with microRNAs become a key component exosomal. Little is known about their involvement in HCV infection mix / HEV or in the early stages of infection, such as in asymptomatic blood donors (BD). To obtain preliminary data, we have compared the microRNA expression profiles in four exosomal each HCV RNA-positive blood donors HEV RNA positive and negative and four patients, one of which is a rare patients with HCV / HEV co-infection.
Exosomes purified from sera by a combination of precipitation and density gradient centrifugation and exosomal microRNA analyzed using the TaqMan array cards. Of the 33 deregulated miRNAs, miR-885-5p and miR-365 regulated in HCV BD, miR-627-5p was downregulated in HCV BD and miR-221 were downregulated in patients with HCV and BD. In HEV infection, miR-526B appears specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) shows potential involvement in both infections. First preliminary data on the treatment of exosomal microRNA profiles before and after antiviral of patients HEV / HCV co-infected reveal puddle 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential role in the infection of this virus.
This is a case-control study including CSF specimens from 41 patients with children. CSF specimens categorized into FS and control groups. microarray tests were conducted to evaluate CSF exosomal miRNA expression profiles. Quantitative PCR (qPCR) assays were performed to validate the microarray assay results. bioinformatics analysis was conducted to analyze the results. Thirteen (62%) patients in group FS experienced a complex FS.
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components)
A total of 96 miRNAs significantly expressed in the study sample CSF and 95 between them, exhibited a higher expression in the FS than the control group. Further validation tests qPCR showed that the top 5 highly expressed miRNA (miR-4486, mir-6850-5p, miR-642b-3p, miR-7107-5p, mir-4281) showed the same results as in the microarray test. bioinformatics analysis identified 455 target genes in the FS group.
