Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

intracranial aneurysm (IA) is a type of cerebrovascular disorders, which can lead to deadly subarachnoid hemorrhage with high handicaps. The purpose of this study was to reveal the pathogenesis and identify novel biomarkers in crude processed IA.We microRNA (miRNA) expression profiling data IA obtained from Gene Expression Omnibus.

Then the weighted correlation network analysis is conducted to identify miRNAs in IA hub. miRNAs target gene hub suspected of using multiR package. In addition, the network of proteins and miRNA-mRNA networks are built and functional and pathway enrichment analysis was performed. Finally, the predictive value of miRNAs in IA tested hubs in set.Two validation module that has a relationship with IA identified 10 miRNAs hub in each module with a gene associated higher-module selected. Network proteins and miRNA-mRNA networks that are 243 nodes and 1496 edges.

Functional and pathway enrichment analysis shows that they are especially enriched in cell cycle, cell proliferation, and PI3K / Akt signaling pathway. Additionally, HSA-miR-191-3p, HSA-miR-423-5p, HSA-miR-424-5p, HSA-miR-425-3p that proved valuable in the prediction IA occurrence.In words, the study revealed hub miRNAs , target genes and pathways potentially participate in the formation and development of IA and screen several biomarker candidates. Our findings provide some new perspectives for research and treatment of IA.

Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

MicroRNAs Exosomal expression profile of HEV- and HCV-Infected Blood Donors and Patients: Pilot Study

Exosomes appears to play an important role in hepatitis C virus (HCV) and hepatitis E virus (HEV) infection to protect their cargo from the host immune response, with microRNAs become a key component exosomal. Little is known about their involvement in HCV infection mix / HEV or in the early stages of infection, such as in asymptomatic blood donors (BD). To obtain preliminary data, we have compared the microRNA expression profiles in four exosomal each HCV RNA-positive blood donors HEV RNA positive and negative and four patients, one of which is a rare patients with HCV / HEV co-infection.

Exosomes purified from sera by a combination of precipitation and density gradient centrifugation and exosomal microRNA analyzed using the TaqMan array cards. Of the 33 deregulated miRNAs, miR-885-5p and miR-365 regulated in HCV BD, miR-627-5p was downregulated in HCV BD and miR-221 were downregulated in patients with HCV and BD. In HEV infection, miR-526B appears specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) shows potential involvement in both infections. First preliminary data on the treatment of exosomal microRNA profiles before and after antiviral of patients HEV / HCV co-infected reveal puddle 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential role in the infection of this virus.


This is a case-control study including CSF specimens from 41 patients with children. CSF specimens categorized into FS and control groups. microarray tests were conducted to evaluate CSF exosomal miRNA expression profiles. Quantitative PCR (qPCR) assays were performed to validate the microarray assay results. bioinformatics analysis was conducted to analyze the results.
Thirteen (62%) patients in group FS experienced a complex FS.

SeraMir Control spike-in Small RNA and qPCR assay

RA805A-1 100 assays
EUR 235

SeraMir Exosome RNA Ampflication Kit

RA800A-1 20 reactions
EUR 914

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999

EZScreen? Glucose Colorimetric Assay Kit (384 Well)

K950-384
EUR 566

EZScreen? Lactate Colorimetric Assay Kit (384 Well)

K951-384
EUR 555

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

RA808A-1 20 preps
EUR 402

Mouse pre-microRNA Expression Construct mir-384

MMIR-384-PA-1 Bacterial Streak
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SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

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SeraMir 3'-Universal Primer (10,000 qPCR reactions)

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Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172

hCRP FRET 384

CH1008-384 1 Kit
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Psoriasis Exosome RNA

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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
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RA806TC-1 10 preps
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PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

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SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
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Total Cholesterol Assay Kit (Colorimetric)

STA-384 192 assays
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LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
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GREENE1-RNA 50pack
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CBA-127 384 assays
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Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2335
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Diabetes (Type 1) Exosome RNA

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GREEN5E100-RNA 100pack
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GREENR1-RNA 50pack
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EUR 181
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Pink RINO RNA Lysis Kit 250

PINKR5-RNA 250pack
EUR 576
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Navy RINO RNA Lysis Kit 250

NAVYR5-RNA 250pack
EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 100

NAVY5E100-RNA 100pack
EUR 453
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

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NAVY5E20-RNA 20pack
EUR 153
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Navy RINO RNA Lysis Kit 50

NAVYR1-RNA 50pack
EUR 202
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5E RED RNA Lysis Kit 100

RED5E100-RNA 100pack
EUR 429
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20

RED5E20-RNA 20pack
EUR 148
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Red RINO RNA Lysis Kit 50

REDR1-RNA 50pack
EUR 195
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250

REDR5-RNA 250pack
EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50

YELLOWR1-RNA 50pack
EUR 199
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250

YELLOWR5-RNA 250pack
EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components)

RA820A-1 20 profiles
EUR 1297

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA812A-1 components)

RA822A-1 20 profiles
EUR 1297

A total of 96 miRNAs significantly expressed in the study sample CSF and 95 between them, exhibited a higher expression in the FS than the control group. Further validation tests qPCR showed that the top 5 highly expressed miRNA (miR-4486, mir-6850-5p, miR-642b-3p, miR-7107-5p, mir-4281) showed the same results as in the microarray test. bioinformatics analysis identified 455 target genes in the FS group.