Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

intracranial aneurysm (IA) is a type of cerebrovascular disorders, which can lead to deadly subarachnoid hemorrhage with high handicaps. The purpose of this study was to reveal the pathogenesis and identify novel biomarkers in crude processed IA.We microRNA (miRNA) expression profiling data IA obtained from Gene Expression Omnibus.

Then the weighted correlation network analysis is conducted to identify miRNAs in IA hub. miRNAs target gene hub suspected of using multiR package. In addition, the network of proteins and miRNA-mRNA networks are built and functional and pathway enrichment analysis was performed. Finally, the predictive value of miRNAs in IA tested hubs in set.Two validation module that has a relationship with IA identified 10 miRNAs hub in each module with a gene associated higher-module selected. Network proteins and miRNA-mRNA networks that are 243 nodes and 1496 edges.

Functional and pathway enrichment analysis shows that they are especially enriched in cell cycle, cell proliferation, and PI3K / Akt signaling pathway. Additionally, HSA-miR-191-3p, HSA-miR-423-5p, HSA-miR-424-5p, HSA-miR-425-3p that proved valuable in the prediction IA occurrence.In words, the study revealed hub miRNAs , target genes and pathways potentially participate in the formation and development of IA and screen several biomarker candidates. Our findings provide some new perspectives for research and treatment of IA.

Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

MicroRNAs Exosomal expression profile of HEV- and HCV-Infected Blood Donors and Patients: Pilot Study

Exosomes appears to play an important role in hepatitis C virus (HCV) and hepatitis E virus (HEV) infection to protect their cargo from the host immune response, with microRNAs become a key component exosomal. Little is known about their involvement in HCV infection mix / HEV or in the early stages of infection, such as in asymptomatic blood donors (BD). To obtain preliminary data, we have compared the microRNA expression profiles in four exosomal each HCV RNA-positive blood donors HEV RNA positive and negative and four patients, one of which is a rare patients with HCV / HEV co-infection.

Exosomes purified from sera by a combination of precipitation and density gradient centrifugation and exosomal microRNA analyzed using the TaqMan array cards. Of the 33 deregulated miRNAs, miR-885-5p and miR-365 regulated in HCV BD, miR-627-5p was downregulated in HCV BD and miR-221 were downregulated in patients with HCV and BD. In HEV infection, miR-526B appears specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) shows potential involvement in both infections. First preliminary data on the treatment of exosomal microRNA profiles before and after antiviral of patients HEV / HCV co-infected reveal puddle 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential role in the infection of this virus.


This is a case-control study including CSF specimens from 41 patients with children. CSF specimens categorized into FS and control groups. microarray tests were conducted to evaluate CSF exosomal miRNA expression profiles. Quantitative PCR (qPCR) assays were performed to validate the microarray assay results. bioinformatics analysis was conducted to analyze the results.
Thirteen (62%) patients in group FS experienced a complex FS.

SeraMir Exosome RNA Ampflication Kit

RA800A-1 20 reactions
EUR 1096.8

SeraMir Control spike-in Small RNA and qPCR assay

RA805A-1 100 assays
EUR 282

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 1198.8

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

RA808A-1 20 preps
EUR 482.4

EZScreen? Glucose Colorimetric Assay Kit (384 Well)

K950-384 each
EUR 679.2

EZScreen? Lactate Colorimetric Assay Kit (384 Well)

K951-384 each
EUR 666

Mouse pre-microRNA Expression Construct mir-384

MMIR-384-PA-1 Bacterial Streak
EUR 820.8

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 664.8

hCRP FRET 384

CH1008-384 1 Kit
EUR 643.2

hCRP TRF 384

CHTR1008-384 1 Kit
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hAlbumin FRET 384

AH1005-384 1 Kit
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hAlbumin TRF 384

AHTR1005-384 1 Kit
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MIGTR1012-384 1 Kit
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hInsulin FRET 384

IH1010-384 1 Kit
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hInsulin TRF 384

IHTR1010-384 1 Kit
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IMTR1009-384 1 Kit
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HITR1011-384 1 Kit
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rIgG FRET 384

RI1015-384 1 Kit
EUR 643.2

SeraMir 3'-Universal Primer (10,000 qPCR reactions)

RA800P-3 10,000 reactions
EUR 213.6

Mouse IgG FRET 384

MIG1012-384 1 Kit
EUR 643.2

Mouse IgM FRET 384

IM1009-384 1 Kit
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Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1406.4

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 664.8

Bridge-It cAMP-PDE 384

PD1007-384 1 Kit
EUR 702

Biotin TRF 384

BTTR-1006-384 1 Kit
EUR 708

384 Well Roche qPCR Plate

PCR0394 PK50
EUR 171

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 1020

Block, 384 well plate, for MultiTherm Touch

H5100-384 1 each
EUR 623

QPCR Kit RNA Coronavirus

MOL6946 EACH
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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 1020

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 790.8

Opti-Seal qPCR 384 Well Plate

G935 10 Plates/Box
EUR 106.8

Opti-Seal qPCR 384 Well Plate

G936 100 Plates/Case
EUR 513.6

384 Well Roche Style qPCR plate

PCR0396 PK50
EUR 255.36

384 WELL PCR MICROPLATE. CLEAR

PCR-384-C 10/pk
EUR 219.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

384 Well Plate, 10/Bag

PCR-384-SN 1PK, 10UNIT
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GREENE1-RNA 50pack
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PINKE1-RNA 50pack
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YELLOW RNA Lysis Kit 250

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PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
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PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
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LiqBiopsy Tumor Exosome RNA Kit

K1254-24 each
EUR 1332

5E Navy RNA Lysis Kit 100

NAVY5E100-RNA 100pack
EUR 543.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

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NAVY5E20-RNA 20pack
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Navy RINO RNA Lysis Kit 50

NAVYR1-RNA 50pack
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250

NAVYR5-RNA 250pack
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Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

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GREEN5E100-RNA 100pack
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GREEN5E20-RNA 20pack
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Green RINO RNA Lysis Kit 50

GREENR1-RNA 50pack
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250

GREENR5-RNA 250pack
EUR 704.4
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E PINK RNA Lysis Kit 100

PINK5E100-RNA 100pack
EUR 456
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20

PINK5E20-RNA 20pack
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Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50

PINKR1-RNA 50pack
EUR 217.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250

PINKR5-RNA 250pack
EUR 691.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100

RED5E100-RNA 100pack
EUR 514.8
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20

RED5E20-RNA 20pack
EUR 177.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50

REDR1-RNA 50pack
EUR 234
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250

REDR5-RNA 250pack
EUR 765.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50

YELLOWR1-RNA 50pack
EUR 238.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250

YELLOWR5-RNA 250pack
EUR 787.2
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Total Cholesterol Assay Kit (Colorimetric)

STA-384 192 assays
EUR 609.6
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.

QPCR Kit RNA Japanese Encep

MOL6358 EACH
EUR 1018.02

QPCR Kit RNA Japanese EncepMmix

MOL6360 EACH
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QPCR Kit RNA Western equine

MOL6362 EACH
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QPCR Kit RNA West NilevirusMmix

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QPCR Kit RNA Rift Valley

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QPCR Kit RNA Tai Forest

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QPCR Kit RNA African Hor

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QPCR Kit RNA Crimean-Con

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QPCR Kit RNA Chaoyang virus

MOL6846 EACH
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QPCR Kit RNA Chaoyang virusMmix

MOL6848 EACH
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QPCR Kit RNA Chikungunya virus

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QPCR Kit RNA Chikungunya viMmix

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QPCR Kit RNA Dengue virus

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QPCR Kit RNA Influenza TypeMmix

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QPCR Kit RNA Human Metap

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QPCR Kit RNA Human Parai

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QPCR Kit RNA Human Parai

MOL8044 EACH
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QPCR Kit RNA Human Parai

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QPCR Kit RNA Human Rhino

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QPCR Kit RNA Human Rhino

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QPCR Kit RNA Human RhinoMmix

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QPCR Kit RNA Human Rhino

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QPCR Kit RNA Mumps virus

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QPCR Kit RNA Mumps virusMmix

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QPCR Kit RNA Respiratory Mmix

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QPCR Kit RNA Rubella virus

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QPCR Kit RNA Rotavirus A

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QPCR Kit RNA Feline coronavirus

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EUR 1097.82

QPCR Kit RNA Feline calicivirus

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EUR 839.04

QPCR Kit RNA Feline calicivirus

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EUR 1097.82

QPCR Kit RNA Feline Leuk

MOL8728 EACH
EUR 839.04

QPCR Kit RNA Feline Immu

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A total of 96 miRNAs significantly expressed in the study sample CSF and 95 between them, exhibited a higher expression in the FS than the control group. Further validation tests qPCR showed that the top 5 highly expressed miRNA (miR-4486, mir-6850-5p, miR-642b-3p, miR-7107-5p, mir-4281) showed the same results as in the microarray test. bioinformatics analysis identified 455 target genes in the FS group.