Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

intracranial aneurysm (IA) is a type of cerebrovascular disorders, which can lead to deadly subarachnoid hemorrhage with high handicaps. The purpose of this study was to reveal the pathogenesis and identify novel biomarkers in crude processed IA.We microRNA (miRNA) expression profiling data IA obtained from Gene Expression Omnibus.

Then the weighted correlation network analysis is conducted to identify miRNAs in IA hub. miRNAs target gene hub suspected of using multiR package. In addition, the network of proteins and miRNA-mRNA networks are built and functional and pathway enrichment analysis was performed. Finally, the predictive value of miRNAs in IA tested hubs in set.Two validation module that has a relationship with IA identified 10 miRNAs hub in each module with a gene associated higher-module selected. Network proteins and miRNA-mRNA networks that are 243 nodes and 1496 edges.

Functional and pathway enrichment analysis shows that they are especially enriched in cell cycle, cell proliferation, and PI3K / Akt signaling pathway. Additionally, HSA-miR-191-3p, HSA-miR-423-5p, HSA-miR-424-5p, HSA-miR-425-3p that proved valuable in the prediction IA occurrence.In words, the study revealed hub miRNAs , target genes and pathways potentially participate in the formation and development of IA and screen several biomarker candidates. Our findings provide some new perspectives for research and treatment of IA.

Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm
Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

MicroRNAs Exosomal expression profile of HEV- and HCV-Infected Blood Donors and Patients: Pilot Study

Exosomes appears to play an important role in hepatitis C virus (HCV) and hepatitis E virus (HEV) infection to protect their cargo from the host immune response, with microRNAs become a key component exosomal. Little is known about their involvement in HCV infection mix / HEV or in the early stages of infection, such as in asymptomatic blood donors (BD). To obtain preliminary data, we have compared the microRNA expression profiles in four exosomal each HCV RNA-positive blood donors HEV RNA positive and negative and four patients, one of which is a rare patients with HCV / HEV co-infection.

Exosomes purified from sera by a combination of precipitation and density gradient centrifugation and exosomal microRNA analyzed using the TaqMan array cards. Of the 33 deregulated miRNAs, miR-885-5p and miR-365 regulated in HCV BD, miR-627-5p was downregulated in HCV BD and miR-221 were downregulated in patients with HCV and BD. In HEV infection, miR-526B appears specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) shows potential involvement in both infections. First preliminary data on the treatment of exosomal microRNA profiles before and after antiviral of patients HEV / HCV co-infected reveal puddle 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential role in the infection of this virus.


This is a case-control study including CSF specimens from 41 patients with children. CSF specimens categorized into FS and control groups. microarray tests were conducted to evaluate CSF exosomal miRNA expression profiles. Quantitative PCR (qPCR) assays were performed to validate the microarray assay results. bioinformatics analysis was conducted to analyze the results.
Thirteen (62%) patients in group FS experienced a complex FS.

Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA811A-1 components) 20 profiles

RA821A-1 20 profiles
EUR 1220

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

RA808A-1 20 preps
EUR 344

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components)

RA820A-1 20 profiles
EUR 1220

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA812A-1 components)

RA822A-1 20 profiles
EUR 1220

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

SeraMir Control spike-in Small RNA and qPCR assay

RA805A-1 100 assays
EUR 181

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 1056

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA820TC-1 20 profiles
EUR 1533

Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA821TC-1 20 profiles
EUR 1533

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 1198.8

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1406.4

SeraMir 3'-Universal Primer (10,000 qPCR reactions)

RA800P-3 10,000 reactions
EUR 125

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 1020

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 1020

Stem Cell MicroRNA Profiling Panel

RA620A-1 10 profiles
EUR 1406.4

rno-miRNome MicroRNA Profiling Kit [Rat]

RA680B-1 20 complete profiles
EUR 2006.4

hsa-miRNome MicroRNA Profiling Kit [Human]

RA660A-1 20 profiles
EUR 2212.8

mmu-miRNome MicroRNA Profiling Kit [Mouse]

RA670A-1 20 profiles
EUR 2046

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1329.6

Psoriasis Exosome RNA

P241-PS - Ask for price

Prostate Cancer Exosome RNA

P241-PR - Ask for price

Lung Cancer Exosome RNA

P241-LG - Ask for price

Normal human exosome RNA

P240 - Ask for price

Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

Leukemia - AML Exosome RNA

P241-AML - Ask for price

Leukemia - CLL Exosome RNA

P241-CLL - Ask for price

Leukemia - CML Exosome RNA

P241-CML - Ask for price

Liver Cancer Exosome RNA

P241-LV - Ask for price

Breast Cancer Exosome RNA

P241-BR - Ask for price

Crohn's Disease Exosome RNA

P241-CR - Ask for price

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Thyroid Cancer Exosome RNA

P241-TH - Ask for price

Colorectal Cancer Exosome RNA

P241-CO - Ask for price

Rheumatoid Arthritis Exosome RNA

P241-RA - Ask for price

Nasopharyngeal Cancer Exosome RNA

P241-NA - Ask for price

Exosome RNA and protein extraction

S103 -
EUR 1200

Diabetes (Type 1) Exosome RNA

P241-DB1 - Ask for price

Diabetes (Type 2) Exosome RNA

P241-DB2 - Ask for price

Leukemia - Hairy Cell Exosome RNA

P241-HC - Ask for price

LiqBiopsy Tumor Exosome RNA Kit

K1254-24 each
EUR 1332

Melanoma (skin cancer) Exosome RNA

P241-MN - Ask for price

Exosome electron microscopy imaging (EM)

S109 -
EUR 600

Uterine Corpus Cancer Exosome RNA

P241-UT - Ask for price

Non-Hodgkin lymphoma Exosome RNA

P241-NH - Ask for price

Uterine Cervical Cancer Exosome RNA

P241-UC - Ask for price

Leukemia - Multiple Myeloma Exosome RNA

P241-MM - Ask for price

Human CD9 Expressed Exosome Detection Assay kit

6036 1 kit
EUR 663.78
Description: Human CD9 Expressed Exosome Detection Assay kit

Human CD63 Expressed Exosome Detection Assay Kit

6037 1 kit
EUR 663.78
Description: Human CD63 Expressed Exosome Detection Assay Kit

Systemic Lupus Erythematosus Exosome RNA

P241-SL - Ask for price

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Human CD9xCD63 Expressed Exosome Detection Assay Kit

6038 1 kit
EUR 377
Description: Culture Media, Serum, Plasma

Exosome RNA Helicase MTR4 (MTR4) Antibody

abx149899-100ug 100 ug
EUR 526.8

Exosome Component 2 (EXOSC2) Magnetic Luminex Assay Kit

LKU603065-96T 96T
EUR 966

Exosome Component 7 (EXOSC7) Magnetic Luminex Assay Kit

LKU603072-96T 96T
EUR 1062.6

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)

M1007-100-T each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, serum, luminometric assay)

M1008-100-W each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, serum, fluorimetric assay)

M1009-100-B each
EUR 888

EVery miRNome Profiler for Human Serum and Plasma

EVery500B-1 8 profiles
EUR 1018

Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

20-abx004268
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1016-100-T each
EUR 874.8

TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1017-100-W each
EUR 888

TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1018-100-B each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1022-100-T each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1023-100-W each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1024-100-B each
EUR 888

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-10 each
EUR 1005.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-20 each
EUR 1546.8

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-3 each
EUR 523.2

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-5 each
EUR 796.8

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1019-100-T each
EUR 907.2

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1020-100-W each
EUR 888

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1021-100-B each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)

M1010-100-T each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)

M1011-100-W each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)

M1012-100-B each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)

M1013-100-T each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)

M1014-100-W each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)

M1015-100-B each
EUR 888

MicroRNA Isolation Kit

KS341025 1 kit
EUR 247

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

K1203-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

K1208-100 each
EUR 1161.6

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay)

M1004-100-T each
EUR 1136.4

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, luminometric assay)

M1005-100-W each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, fluorometric assay)

M1006-100-B each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1025-100-T each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1026-100-W each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1027-100-B each
EUR 888

Human microRNA-210(miR-210)ELISA

QY-E05678 96T
EUR 433.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)

M1039-10 each
EUR 1005.6

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions)

M1039-20 each
EUR 1546.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)

M1039-3 each
EUR 523.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 5 reactions)

M1039-5 each
EUR 796.8

Plant MicroRNA Purification Kit

10760135-1 25 Prep(s)
EUR 245.48

Human microrna-21(mir-21) ELISA Kit

SL3405Hu 96 Tests
EUR 468

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1030-10 each
EUR 1005.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1030-20 each
EUR 1546.8

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1030-3 each
EUR 529.2

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)

M1030-5 each
EUR 796.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1031-10 each
EUR 1005.6

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1031-20 each
EUR 1546.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1031-3 each
EUR 523.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)

M1031-5 each
EUR 796.8

MicroRNA scramble in CD511 - Virus

PMIRH000VA-1 1x10^6 IFUs
EUR 715

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

K1210-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

K1205-100 each
EUR 1161.6

Exosome (Pre-isolated) RNA Extraction kit Note! This kit doesn't contain the conponents for exosome enrichment.

EIRK-01 50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

K1206-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100 each
EUR 1161.6

Opti-Seal qPCR Roche 480 (Low profile)

G913 100 Plates/Case
EUR 362.4

Opti-Seal qPCR Roche 480 (Low profile)

G914 10 Plates/Box
EUR 91.2

SanPrep Column microRNA Miniprep Kit

SK8811 50prep
EUR 151.32

Total Exosome RNA Extraction Kit (Biological Fluids/Culture Media Supplement)

EIRK-03 20 reactions
EUR 3380.4
Description: This product is a useful tool for the isolation and efficient total RNA (miRNA and mRNAs) extraction from the total exosome population from human biofluids or cell culture media.

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100 each
EUR 1161.6

MicroRNA Precursor Pooled Plasmid Library

PMIRHPLPA-1 1 aliquot plasmid DNA
EUR 60

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1032-10 each
EUR 1057.2

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

M1032-20 each
EUR 1618.8

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)

M1032-3 each
EUR 523.2

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)

M1032-5 each
EUR 796.8

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1033-10 each
EUR 1057.2

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

M1033-20 each
EUR 1618.8

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)

M1033-3 each
EUR 523.2

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)

M1033-5 each
EUR 796.8

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)

M1034-10 each
EUR 1005.6

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions)

M1034-20 each
EUR 1495.2

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)

M1034-3 each
EUR 398.4

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 5 reactions)

M1034-5 each
EUR 777.6

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)

M1035-10 each
EUR 1005.6

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions)

M1035-20 each
EUR 1495.2

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)

M1035-3 each
EUR 516

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 5 reactions)

M1035-5 each
EUR 777.6

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 10 reactions)

M1036-10 each
EUR 1005.6

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 20 reactions)

M1036-20 each
EUR 1546.8

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)

M1036-3 each
EUR 516

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 5 reactions)

M1036-5 each
EUR 777.6

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 10 reactions)

M1037-10 each
EUR 1005.6

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 20 reactions)

M1037-20 each
EUR 1546.8

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)

M1037-3 each
EUR 516

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 5 reactions)

M1037-5 each
EUR 777.6

miRZip-1 anti-miR-1 microRNA construct

MZIP1-PA-1 Bacterial Streak
EUR 620

miRZip-7 anti-miR-7 microRNA construct

MZIP7-PA-1 Bacterial Streak
EUR 620

miRZip-9 anti-miR-9 microRNA construct

MZIP9-PA-1 Bacterial Streak
EUR 620

miRZip-16 anti-miR-16 microRNA construct

MZIP16-PA-1 Bacterial Streak
EUR 620

miRZip-17 anti-miR-17 microRNA construct

MZIP17-AA-1 Miniprep DNA
EUR 620

miRZip-17 anti-miR-17 microRNA construct

MZIP17-PA-1 Bacterial Streak
EUR 620

miRZip-21 anti-miR-21 microRNA construct

MZIP21-PA-1 Bacterial Streak
EUR 620

miRZip-22 anti-miR-22 microRNA construct

MZIP22-PA-1 Bacterial Streak
EUR 620

miRZip-24 anti-miR-24 microRNA construct

MZIP24-PA-1 Bacterial Streak
EUR 620

miRZip-25 anti-miR-25 microRNA construct

MZIP25-PA-1 Bacterial Streak
EUR 620

miRZip-31 anti-miR-31 microRNA construct

MZIP31-PA-1 Bacterial Streak
EUR 620

miRZip-32 anti-miR-32 microRNA construct

MZIP32-PA-1 Bacterial Streak
EUR 620

miRZip-93 anti-miR-93 microRNA construct

MZIP93-PA-1 Bacterial Streak
EUR 620

A total of 96 miRNAs significantly expressed in the study sample CSF and 95 between them, exhibited a higher expression in the FS than the control group. Further validation tests qPCR showed that the top 5 highly expressed miRNA (miR-4486, mir-6850-5p, miR-642b-3p, miR-7107-5p, mir-4281) showed the same results as in the microarray test. bioinformatics analysis identified 455 target genes in the FS group.