Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles

Background: There is a significant variability in weight loss after bariatric surgery. We hypothesize that part of this variability can be predetermined by the genetic differences associated with metabolic homeostasis. MicroRNA (miRNA) are short pieces of RNA that regulate gene expression and easily detectable in the serum. They are involved in many metabolic processes, including weight homeostasis. In this pilot study, we briefly review the role of miRNA, and assess the feasibility of using them in a clinical setting treatment of obesity.


Objective: To evaluate the feasibility of using miRNA to predict weight loss after bariatric surgery.


Setting: Academic medical center.


Methods: Serum collected from patients at the initial bariatric surgery consultation. Weight loss data were collected 6 and 12 months postoperatively. Individuals experiencing a bit and the largest number of percentage of excess weight lost at 6 months were analyzed to assess genetic differences in miRNA expression.


Results: The median percentage of excess weight lost was 51% (range, 34% -63%) for those who lose the least and 87% (range, 82% -111%) for those who lost the most weight. Groups were similar in age, gender, diabetes status, and type of operation. In total, of 119 miRNA is detected in the serum of patients, 6 showed potential to differentiate between weight loss group high and low. Mirna has previously been involved in the regulation of fatty acid biosynthesis, adipocytes proliferation, type 2 diabetes, and obesity.


Conclusions: In this pilot study, we demonstrate the feasibility of identifying genetic differences between groups of weight loss by identifying high and low serum miRNA different. In the near future, these biomarkers could facilitate decisions about surgery. In addition, this miRNA could open up new genetic pathways that describe the pathophysiology of obesity, and provide targets for future treatment.

Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles
Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles

The platelet microRNA Profile Kawasaki Disease: Identification of Novel Diagnostic Biomarkers

Challenging the unknown diagnosis and etiology of Kawasaki disease (KD) increases the incidence of coronary artery lesions. microRNAs (miRNAs) are the most promising biomarker for their stability in peripheral blood and noninvasive measurement procedures, which have proven potential utility in cancer. To explore the utility expressed differently (DE) miRNAs early diagnostic markers, 44 patients (25 KD 19, KD incomplete and complete) and 31 controls were recruited fever for small RNA sequencing.

Of all the states in 1922 Mirna, DE 210 miRNAs were found between KD and control groups fever. Although platelet miRNA profile of a complete full KD KD similar through cluster analysis, DE miRNAs are not identical. DE eight miRNAs validated by quantitative real-time PCR (qRT-PCR) in a group of complete or incomplete KD using a normalizer, miR-126-3p, identified by geNorm and NormFinder tools.

SeraMir Control spike-in Small RNA and qPCR assay

RA805A-1 100 assays
EUR 235
  • Category: MicroRNA Tools

SeraMir Exosome RNA Ampflication Kit

RA800A-1 20 reactions
EUR 914
  • Category: MicroRNA Tools

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999
  • Category: Lineage Profilers

EZScreen? Glucose Colorimetric Assay Kit (384 Well)

K950-384
EUR 566

EZScreen? Lactate Colorimetric Assay Kit (384 Well)

K951-384
EUR 555

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

RA808A-1 20 preps
EUR 402
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct mir-384

MMIR-384-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 554
  • Category: MicroRNA Tools

SeraMir 3'-Universal Primer (10,000 qPCR reactions)

RA800P-3 10,000 reactions
EUR 178
  • Category: MicroRNA Tools

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172
  • Category: MicroRNA Tools

hCRP FRET 384

CH1008-384 1 Kit
EUR 536

hCRP TRF 384

CHTR1008-384 1 Kit
EUR 590

hAlbumin FRET 384

AH1005-384 1 Kit
EUR 536

hAlbumin TRF 384

AHTR1005-384 1 Kit
EUR 590

hIgG TRF 384

HITR1011-384 1 Kit
EUR 590

hInsulin FRET 384

IH1010-384 1 Kit
EUR 536

hInsulin TRF 384

IHTR1010-384 1 Kit
EUR 590

mIgM TRF 384

IMTR1009-384 1 Kit
EUR 590

mIgG TRF 384

MIGTR1012-384 1 Kit
EUR 590

rIgG FRET 384

RI1015-384 1 Kit
EUR 536

Mouse IgM FRET 384

IM1009-384 1 Kit
EUR 536

Mouse IgG FRET 384

MIG1012-384 1 Kit
EUR 536

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

Psoriasis Exosome RNA

P241-PS NULL
EUR 0

Opti-Seal qPCR 384 Well Plate

G935 10 Plates/Box
EUR 89

Opti-Seal qPCR 384 Well Plate

G936 100 Plates/Case
EUR 428

Bridge-It cAMP-PDE 384

PD1007-384 1 Kit
EUR 585

Biotin TRF 384

BTTR-1006-384 1 Kit
EUR 590

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 554
  • Category: MicroRNA Tools

Normal human exosome RNA

P240 NULL
EUR 0

Leukemia - ALL Exosome RNA

P241-ALL NULL
EUR 0

Leukemia - AML Exosome RNA

P241-AML NULL
EUR 0

Bladder Cancer Exosome RNA

P241-BL NULL
EUR 0

Breast Cancer Exosome RNA

P241-BR NULL
EUR 0

Leukemia - CLL Exosome RNA

P241-CLL NULL
EUR 0

Leukemia - CML Exosome RNA

P241-CML NULL
EUR 0

Colorectal Cancer Exosome RNA

P241-CO NULL
EUR 0

Crohn's Disease Exosome RNA

P241-CR NULL
EUR 0

Kidney Cancer Exosome RNA

P241-KN NULL
EUR 0

Lung Cancer Exosome RNA

P241-LG NULL
EUR 0

Liver Cancer Exosome RNA

P241-LV NULL
EUR 0

Nasopharyngeal Cancer Exosome RNA

P241-NA NULL
EUR 0

Prostate Cancer Exosome RNA

P241-PR NULL
EUR 0

Rheumatoid Arthritis Exosome RNA

P241-RA NULL
EUR 0

Thyroid Cancer Exosome RNA

P241-TH NULL
EUR 0

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

384 WELL PCR MICROPLATE. CLEAR

PCR-384-C 10/pk
EUR 183
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

384 Well Plate, 10/Bag

PCR-384-SN 1PK, 10UNIT
EUR 93.5
  • Product category: Labware/Multiwell Plates/384-Well

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 659
  • Category: MicroRNA Tools

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA812A-1 components)

RA822A-1 20 profiles
EUR 1297
  • Category: MicroRNA Tools

Total Cholesterol Assay Kit (Colorimetric)

STA-384 192 assays
EUR 508
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

LiqBiopsy Tumor Exosome RNA Kit

K1254-24
EUR 1110

Diabetes (Type 1) Exosome RNA

P241-DB1 NULL
EUR 0

Diabetes (Type 2) Exosome RNA

P241-DB2 NULL
EUR 0

Leukemia - Hairy Cell Exosome RNA

P241-HC NULL
EUR 0

Leukemia - Multiple Myeloma Exosome RNA

P241-MM NULL
EUR 0

Melanoma (skin cancer) Exosome RNA

P241-MN NULL
EUR 0

Non-Hodgkin lymphoma Exosome RNA

P241-NH NULL
EUR 0

Systemic Lupus Erythematosus Exosome RNA

P241-SL NULL
EUR 0

Uterine Cervical Cancer Exosome RNA

P241-UC NULL
EUR 0

Uterine Corpus Cancer Exosome RNA

P241-UT NULL
EUR 0

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 601
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2335
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS NULL
EUR 0

5E GREEN RNA Lysis Kit 100

GREEN5E100-RNA 100pack
EUR 386
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20

GREEN5E20-RNA 20pack
EUR 138
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50

GREENR1-RNA 50pack
EUR 184
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250

GREENR5-RNA 250pack
EUR 587
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E Navy RNA Lysis Kit 100

NAVY5E100-RNA 100pack
EUR 453
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20

NAVY5E20-RNA 20pack
EUR 153
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50

NAVYR1-RNA 50pack
EUR 202
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250

NAVYR5-RNA 250pack
EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E PINK RNA Lysis Kit 100

PINK5E100-RNA 100pack
EUR 380
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20

PINK5E20-RNA 20pack
EUR 136
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50

PINKR1-RNA 50pack
EUR 181
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250

PINKR5-RNA 250pack
EUR 576
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100

RED5E100-RNA 100pack
EUR 429
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20

RED5E20-RNA 20pack
EUR 148
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50

REDR1-RNA 50pack
EUR 195
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250

REDR5-RNA 250pack
EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50

YELLOWR1-RNA 50pack
EUR 199
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250

YELLOWR5-RNA 250pack
EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components)

RA820A-1 20 profiles
EUR 1297
  • Category: MicroRNA Tools

50UL 384 WELL PIPET TIPS FOR FINNPIPETTE PIPETTORS

FPT-384-R 3840/pk
EUR 2109
Description: Robotic Tips; Robotic tips - Axygen

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1007
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 3901
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

EZScreen? ATP Colorimetric Assay Kit (384-well)

K959-400
EUR 593

EZScreen? Glycogen Colorimetric Assay Kit (384-Well)

K960-400
EUR 648

Exosome RNA Helicase MTR4 (MTR4) Antibody

abx149899-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402

Human pre-microRNA Expression Construct Lenti-miR-384

PMIRH384PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Human CD9 Expressed Exosome Detection Assay kit

6036 1 kit
EUR 553.15
Description: Human CD9 Expressed Exosome Detection Assay kit

Human CD63 Expressed Exosome Detection Assay Kit

6037 1 kit
EUR 553.15
Description: Human CD63 Expressed Exosome Detection Assay Kit

TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1016-100-T
EUR 729

TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1017-100-W
EUR 740

TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1018-100-B
EUR 740

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1019-100-T
EUR 756

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1020-100-W
EUR 740

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1021-100-B
EUR 740

GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1022-100-T
EUR 740

GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1023-100-W
EUR 740

GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1024-100-B
EUR 740

384 WELL BLACK RIGI PLATE PCR MICROPLATE

PCR-384-RGD-BK 20/pk
EUR 488
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA811A-1 components) 20 profiles

RA821A-1 20 profiles
EUR 1297
  • Category: MicroRNA Tools

Equalbit RNA HS Assay Kit

EQ211-01 100 rxn
EUR 203

Equalbit RNA HS Assay Kit

EQ211-02 500 rxn
EUR 387

Equalbit RNA BR Assay Kit

EQ212-01 100 rxn
EUR 203

Equalbit RNA BR Assay Kit

EQ212-02 500 rxn
EUR 387

EZScreen? Triglyceride Quantification Colorimetric Assay Kit (384-well)

K952-400
EUR 626

EZScreen? NAD+/NADH Colorimetric Assay Kit (384-well)

K958-400
EUR 648

Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

20-abx004268
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR

PCR-384-RGD-C 20/pk
EUR 459
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)

M1007-100-T
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, serum, luminometric assay)

M1008-100-W
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, serum, fluorimetric assay)

M1009-100-B
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)

M1010-100-T
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)

M1011-100-W
EUR 740

The expression levels of miRNAs are continually changing over time was observed and analysis of miRNAs function suggests a potential role as a therapeutic biomarker. In addition, a prediction model for KD showed 78.8% sensitivity and 71.4% specificity, respectively. This study uses small RNA sequencing to identify miRNA biomarkers KD diagnosis is based on a large sample size. Our findings illuminate understanding of the molecular pathogenesis of KD and can improve the accuracy of diagnosis and prognosis in clinical KD.