Background: There is a significant variability in weight loss after bariatric surgery. We hypothesize that part of this variability can be predetermined by the genetic differences associated with metabolic homeostasis. MicroRNA (miRNA) are short pieces of RNA that regulate gene expression and easily detectable in the serum. They are involved in many metabolic processes, including weight homeostasis. In this pilot study, we briefly review the role of miRNA, and assess the feasibility of using them in a clinical setting treatment of obesity.

Objective: To evaluate the feasibility of using miRNA to predict weight loss after bariatric surgery.

Setting: Academic medical center.

Methods: Serum collected from patients at the initial bariatric surgery consultation. Weight loss data were collected 6 and 12 months postoperatively. Individuals experiencing a bit and the largest number of percentage of excess weight lost at 6 months were analyzed to assess genetic differences in miRNA expression.

Results: The median percentage of excess weight lost was 51% (range, 34% -63%) for those who lose the least and 87% (range, 82% -111%) for those who lost the most weight. Groups were similar in age, gender, diabetes status, and type of operation. In total, of 119 miRNA is detected in the serum of patients, 6 showed potential to differentiate between weight loss group high and low. Mirna has previously been involved in the regulation of fatty acid biosynthesis, adipocytes proliferation, type 2 diabetes, and obesity.

Conclusions: In this pilot study, we demonstrate the feasibility of identifying genetic differences between groups of weight loss by identifying high and low serum miRNA different. In the near future, these biomarkers could facilitate decisions about surgery. In addition, this miRNA could open up new genetic pathways that describe the pathophysiology of obesity, and provide targets for future treatment.

The platelet microRNA Profile Kawasaki Disease: Identification of Novel Diagnostic Biomarkers

Challenging the unknown diagnosis and etiology of Kawasaki disease (KD) increases the incidence of coronary artery lesions. microRNAs (miRNAs) are the most promising biomarker for their stability in peripheral blood and noninvasive measurement procedures, which have proven potential utility in cancer. To explore the utility expressed differently (DE) miRNAs early diagnostic markers, 44 patients (25 KD 19, KD incomplete and complete) and 31 controls were recruited fever for small RNA sequencing.

Of all the states in 1922 Mirna, DE 210 miRNAs were found between KD and control groups fever. Although platelet miRNA profile of a complete full KD KD similar through cluster analysis, DE miRNAs are not identical. DE eight miRNAs validated by quantitative real-time PCR (qRT-PCR) in a group of complete or incomplete KD using a normalizer, miR-126-3p, identified by geNorm and NormFinder tools.

EZScreen? Lactate Colorimetric Assay Kit (384 Well)
K951-384	Biovision	each	EUR 666

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)
RA808A-1	SBI	20 preps	EUR 482.4

Mouse pre-microRNA Expression Construct mir-384
MMIR-384-PA-1	SBI	Bacterial Streak	EUR 820.8

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1	SBI	20 preps	EUR 664.8

hAlbumin FRET 384
AH1005-384	Mediomics	1 Kit	EUR 643.2

hAlbumin TRF 384
AHTR1005-384	Mediomics	1 Kit	EUR 708

hCRP FRET 384
CH1008-384	Mediomics	1 Kit	EUR 643.2

hCRP TRF 384
CHTR1008-384	Mediomics	1 Kit	EUR 708

mIgG TRF 384
MIGTR1012-384	Mediomics	1 Kit	EUR 708

hIgG TRF 384
HITR1011-384	Mediomics	1 Kit	EUR 708

hInsulin FRET 384
IH1010-384	Mediomics	1 Kit	EUR 643.2

hInsulin TRF 384
IHTR1010-384	Mediomics	1 Kit	EUR 708

mIgM TRF 384
IMTR1009-384	Mediomics	1 Kit	EUR 708

rIgG FRET 384
RI1015-384	Mediomics	1 Kit	EUR 643.2

Mouse IgG FRET 384
MIG1012-384	Mediomics	1 Kit	EUR 643.2

Mouse IgM FRET 384
IM1009-384	Mediomics	1 Kit	EUR 643.2

SeraMir 3'-Universal Primer (10,000 qPCR reactions)
RA800P-3	SBI	10,000 reactions	EUR 213.6

Cancer MicroRNA qPCR Array
RA610A-1	SBI	10 profiles	EUR 1406.4

Bridge-It cAMP-PDE 384
PD1007-384	Mediomics	1 Kit	EUR 702

Biotin TRF 384
BTTR-1006-384	Mediomics	1 Kit	EUR 708

384 Well Roche qPCR Plate
PCR0394	Scientific Laboratory Supplies	PK50	EUR 171

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1	SBI	10 preps	EUR 664.8

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA900A-1	SBI	20 profiles	EUR 1020

Block, 384 well plate, for MultiTherm Touch
H5100-384	Benchmark Scientific	1 each	EUR 623

QPCR Kit RNA Coronavirus
MOL6946	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA Respiratory
MOL8300	Scientific Laboratory Supplies	EACH	EUR 1220.94

QPCR Kit RNA Respiratory
MOL8304	Scientific Laboratory Supplies	EACH	EUR 1220.94

QPCR Kit RNA Respiratory
MOL8308	Scientific Laboratory Supplies	EACH	EUR 1220.94

QPCR Kit RNA H10N8
MOL8488	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA H10N8Mmix
MOL8490	Scientific Laboratory Supplies	EACH	EUR 1027.14

QPCR Kit RNA Infectious
MOL8780	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Infectious
MOL8788	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Vesivirus2117
MOL8908	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Vesivirus2117Mmix
MOL8910	Scientific Laboratory Supplies	EACH	EUR 1010.04

Opti-Seal qPCR 384 Well Plate
G935	ABM	10 Plates/Box	EUR 106.8

Opti-Seal qPCR 384 Well Plate
G936	ABM	100 Plates/Case	EUR 513.6

384 Well Roche Style qPCR plate
PCR0396	Scientific Laboratory Supplies	PK50	EUR 255.36

384 WELL PCR MICROPLATE. CLEAR
PCR-384-C	CORNING	10/pk	EUR 219.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

384 Well Plate, 10/Bag
PCR-384-SN	Bio Basic	1PK, 10UNIT	EUR 112.2

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA930A-1	SBI	20 profiles	EUR 1020

Navy RNA Lysis Kit 50
NAVYE1-RNA	Next Advance	50pack	EUR 237.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250
NAVYE5-RNA	Next Advance	250pack	EUR 781.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Green RNA Lysis Kit 50
GREENE1-RNA	Next Advance	50pack	EUR 216
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250
GREENE5-RNA	Next Advance	250pack	EUR 685.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Pink RNA Lysis Kit 50
PINKE1-RNA	Next Advance	50pack	EUR 213.6
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250
PINKE5-RNA	Next Advance	250pack	EUR 673.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50
REDE1-RNA	Next Advance	50pack	EUR 230.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250
REDE5-RNA	Next Advance	250pack	EUR 746.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50
YELLOWE1-RNA	Next Advance	50pack	EUR 230.4
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250
YELLOWE5-RNA	Next Advance	250pack	EUR 748.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1	SBI	10 reactions	EUR 790.8

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA812A-1 components)
RA822A-1	SBI	20 profiles	EUR 1556.4

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500
HS-100-QPCR	CORNING	100/pk	EUR 381.6
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500
HS-150-QPCR	CORNING	100/pk	EUR 381.6
Description: Sealing Products; Sealing films - Axygen

LiqBiopsy Tumor Exosome RNA Kit
K1254-24	Biovision	each	EUR 1332

5E Navy RNA Lysis Kit 100
NAVY5E100-RNA	Next Advance	100pack	EUR 543.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20
NAVY5E20-RNA	Next Advance	20pack	EUR 183.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50
NAVYR1-RNA	Next Advance	50pack	EUR 242.4
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250
NAVYR5-RNA	Next Advance	250pack	EUR 799.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E GREEN RNA Lysis Kit 100
GREEN5E100-RNA	Next Advance	100pack	EUR 463.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20
GREEN5E20-RNA	Next Advance	20pack	EUR 165.6
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50
GREENR1-RNA	Next Advance	50pack	EUR 220.8
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250
GREENR5-RNA	Next Advance	250pack	EUR 704.4
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E PINK RNA Lysis Kit 100
PINK5E100-RNA	Next Advance	100pack	EUR 456
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20
PINK5E20-RNA	Next Advance	20pack	EUR 163.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50
PINKR1-RNA	Next Advance	50pack	EUR 217.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250
PINKR5-RNA	Next Advance	250pack	EUR 691.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100
RED5E100-RNA	Next Advance	100pack	EUR 514.8
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20
RED5E20-RNA	Next Advance	20pack	EUR 177.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50
REDR1-RNA	Next Advance	50pack	EUR 234
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250
REDR5-RNA	Next Advance	250pack	EUR 765.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50
YELLOWR1-RNA	Next Advance	50pack	EUR 238.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250
YELLOWR5-RNA	Next Advance	250pack	EUR 787.2
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Total Cholesterol Assay Kit (Colorimetric)
STA-384	Cell Biolabs	192 assays	EUR 609.6
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.

QPCR Kit RNA Japanese Encep
MOL6358	Scientific Laboratory Supplies	EACH	EUR 1018.02

QPCR Kit RNA Japanese EncepMmix
MOL6360	Scientific Laboratory Supplies	EACH	EUR 1273.38

QPCR Kit RNA Western equine
MOL6362	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA Western equineMmix
MOL6364	Scientific Laboratory Supplies	EACH	EUR 1051.08

QPCR Kit RNA West NilevirusMmix
MOL6368	Scientific Laboratory Supplies	EACH	EUR 1097.82

QPCR Kit RNA Rift Valley
MOL6394	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Tai Forest
MOL6402	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA African Hor
MOL6406	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Norovirus g
MOL6662	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Crimean-Con
MOL6706	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Chaoyang virus
MOL6846	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Chaoyang virusMmix
MOL6848	Scientific Laboratory Supplies	EACH	EUR 1010.04

QPCR Kit RNA Chikungunya virus
MOL6850	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Chikungunya viMmix
MOL6852	Scientific Laboratory Supplies	EACH	EUR 1097.82

QPCR Kit RNA Dengue virus
MOL6858	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Dengue virusMmix
MOL6860	Scientific Laboratory Supplies	EACH	EUR 1097.82

QPCR Kit RNA Influenza TypeMmix
MOL6900	Scientific Laboratory Supplies	EACH	EUR 1476.3

QPCR Kit RNA Human Influ
MOL6902	Scientific Laboratory Supplies	EACH	EUR 1220.94

QPCR Kit RNA Human Influ
MOL6906	Scientific Laboratory Supplies	EACH	EUR 1220.94

QPCR Kit RNA H1N1 influenza
MOL6930	Scientific Laboratory Supplies	EACH	EUR 1220.94

QPCR Kit RNA H1N1 influenzaMmix
MOL6932	Scientific Laboratory Supplies	EACH	EUR 1733.94

QPCR Kit RNA Human Influ
MOL6934	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Coronavirus Mmix
MOL6948	Scientific Laboratory Supplies	EACH	EUR 1051.08

QPCR Kit RNA Hepatitis D
MOL6962	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Human Immun
MOL6994	Scientific Laboratory Supplies	EACH	EUR 864.12

QPCR Kit RNA Human Immun
MOL6998	Scientific Laboratory Supplies	EACH	EUR 864.12

QPCR Kit RNA Human Metap
MOL8024	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Human Parai
MOL8032	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA Human Parai
MOL8036	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA Human Parai
MOL8040	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA Human Parai
MOL8044	Scientific Laboratory Supplies	EACH	EUR 840.18

QPCR Kit RNA Human Parai
MOL8048	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Human Rhino
MOL8108	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA Human Rhino
MOL8112	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Human Rhino
MOL8116	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Human RhinoMmix
MOL8118	Scientific Laboratory Supplies	EACH	EUR 1010.04

QPCR Kit RNA Human Rhino
MOL8120	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Human Rhino
MOL8124	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Human Rhino
MOL8128	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Mumps virus
MOL8240	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Mumps virusMmix
MOL8242	Scientific Laboratory Supplies	EACH	EUR 1010.04

QPCR Kit RNA Respiratory Mmix
MOL8302	Scientific Laboratory Supplies	EACH	EUR 1476.3

QPCR Kit RNA Respiratory Mmix
MOL8306	Scientific Laboratory Supplies	EACH	EUR 1476.3

QPCR Kit RNA Respiratory Mmix
MOL8310	Scientific Laboratory Supplies	EACH	EUR 1476.3

QPCR Kit RNA Rubella virus
MOL8312	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Rubella virusMmix
MOL8314	Scientific Laboratory Supplies	EACH	EUR 1010.04

QPCR Kit RNA Sandfly Fev
MOL8332	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Tick-borne
MOL8376	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Dobrava-Bel
MOL8472	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Bird flu
MOL8492	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Bird fluMmix
MOL8494	Scientific Laboratory Supplies	EACH	EUR 1341.78

QPCR Kit RNA Avian Influ
MOL8496	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Rabies virus
MOL8512	Scientific Laboratory Supplies	EACH	EUR 820.8

QPCR Kit RNA Rabies virusMmix
MOL8514	Scientific Laboratory Supplies	EACH	EUR 1051.08

QPCR Kit RNA Rotavirus A
MOL8516	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Rotavirus AMmix
MOL8518	Scientific Laboratory Supplies	EACH	EUR 1097.82

The expression levels of miRNAs are continually changing over time was observed and analysis of miRNAs function suggests a potential role as a therapeutic biomarker. In addition, a prediction model for KD showed 78.8% sensitivity and 71.4% specificity, respectively. This study uses small RNA sequencing to identify miRNA biomarkers KD diagnosis is based on a large sample size. Our findings illuminate understanding of the molecular pathogenesis of KD and can improve the accuracy of diagnosis and prognosis in clinical KD.