Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles

Background: There is a significant variability in weight loss after bariatric surgery. We hypothesize that part of this variability can be predetermined by the genetic differences associated with metabolic homeostasis. MicroRNA (miRNA) are short pieces of RNA that regulate gene expression and easily detectable in the serum. They are involved in many metabolic processes, including weight homeostasis. In this pilot study, we briefly review the role of miRNA, and assess the feasibility of using them in a clinical setting treatment of obesity.


Objective: To evaluate the feasibility of using miRNA to predict weight loss after bariatric surgery.


Setting: Academic medical center.


Methods: Serum collected from patients at the initial bariatric surgery consultation. Weight loss data were collected 6 and 12 months postoperatively. Individuals experiencing a bit and the largest number of percentage of excess weight lost at 6 months were analyzed to assess genetic differences in miRNA expression.


Results: The median percentage of excess weight lost was 51% (range, 34% -63%) for those who lose the least and 87% (range, 82% -111%) for those who lost the most weight. Groups were similar in age, gender, diabetes status, and type of operation. In total, of 119 miRNA is detected in the serum of patients, 6 showed potential to differentiate between weight loss group high and low. Mirna has previously been involved in the regulation of fatty acid biosynthesis, adipocytes proliferation, type 2 diabetes, and obesity.


Conclusions: In this pilot study, we demonstrate the feasibility of identifying genetic differences between groups of weight loss by identifying high and low serum miRNA different. In the near future, these biomarkers could facilitate decisions about surgery. In addition, this miRNA could open up new genetic pathways that describe the pathophysiology of obesity, and provide targets for future treatment.

Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles
Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles

The platelet microRNA Profile Kawasaki Disease: Identification of Novel Diagnostic Biomarkers

Challenging the unknown diagnosis and etiology of Kawasaki disease (KD) increases the incidence of coronary artery lesions. microRNAs (miRNAs) are the most promising biomarker for their stability in peripheral blood and noninvasive measurement procedures, which have proven potential utility in cancer. To explore the utility expressed differently (DE) miRNAs early diagnostic markers, 44 patients (25 KD 19, KD incomplete and complete) and 31 controls were recruited fever for small RNA sequencing.

Of all the states in 1922 Mirna, DE 210 miRNAs were found between KD and control groups fever. Although platelet miRNA profile of a complete full KD KD similar through cluster analysis, DE miRNAs are not identical. DE eight miRNAs validated by quantitative real-time PCR (qRT-PCR) in a group of complete or incomplete KD using a normalizer, miR-126-3p, identified by geNorm and NormFinder tools.

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
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SeraMir Control spike-in Small RNA and qPCR assay

RA805A-1 100 assays
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Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
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SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
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Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA820TC-1 20 profiles
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Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA821TC-1 20 profiles
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SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 1198.8

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1406.4

SeraMir 3'-Universal Primer (10,000 qPCR reactions)

RA800P-3 10,000 reactions
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rno-miRNome MicroRNA Profiling Kit [Rat]

RA680B-1 20 complete profiles
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LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
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Stem Cell MicroRNA Profiling Panel

RA620A-1 10 profiles
EUR 1406.4

hsa-miRNome MicroRNA Profiling Kit [Human]

RA660A-1 20 profiles
EUR 2212.8

mmu-miRNome MicroRNA Profiling Kit [Mouse]

RA670A-1 20 profiles
EUR 2046

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
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Psoriasis Exosome RNA

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Normal human exosome RNA

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Leukemia - AML Exosome RNA

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Leukemia - CLL Exosome RNA

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Liver Cancer Exosome RNA

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Breast Cancer Exosome RNA

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Crohn's Disease Exosome RNA

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Kidney Cancer Exosome RNA

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Bladder Cancer Exosome RNA

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Thyroid Cancer Exosome RNA

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Colorectal Cancer Exosome RNA

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Rheumatoid Arthritis Exosome RNA

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Nasopharyngeal Cancer Exosome RNA

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Exosome RNA and protein extraction

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Diabetes (Type 1) Exosome RNA

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Diabetes (Type 2) Exosome RNA

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Leukemia - Hairy Cell Exosome RNA

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LiqBiopsy Tumor Exosome RNA Kit

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Melanoma (skin cancer) Exosome RNA

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Exosome electron microscopy imaging (EM)

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Uterine Corpus Cancer Exosome RNA

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Non-Hodgkin lymphoma Exosome RNA

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Uterine Cervical Cancer Exosome RNA

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Leukemia - Multiple Myeloma Exosome RNA

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Human CD9 Expressed Exosome Detection Assay kit

6036 1 kit
EUR 663.78
Description: Human CD9 Expressed Exosome Detection Assay kit

Human CD63 Expressed Exosome Detection Assay Kit

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Description: Human CD63 Expressed Exosome Detection Assay Kit

Systemic Lupus Erythematosus Exosome RNA

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Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

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Human CD9xCD63 Expressed Exosome Detection Assay Kit

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Description: Culture Media, Serum, Plasma

Exosome RNA Helicase MTR4 (MTR4) Antibody

abx149899-100ug 100 ug
EUR 526.8

Exosome Component 2 (EXOSC2) Magnetic Luminex Assay Kit

LKU603065-96T 96T
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Exosome Component 7 (EXOSC7) Magnetic Luminex Assay Kit

LKU603072-96T 96T
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ExoQuick Exosome RNA Column Purification kit

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ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)

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EVery miRNome Profiler for Human Serum and Plasma

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Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

20-abx004268
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
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  • 50 ul

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MicroRNA Isolation Kit

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

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EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

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Plant MicroRNA Purification Kit

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Human microrna-21(mir-21) ELISA Kit

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MicroRNA scramble in CD511 - Virus

PMIRH000VA-1 1x10^6 IFUs
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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

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Exosome (Pre-isolated) RNA Extraction kit Note! This kit doesn't contain the conponents for exosome enrichment.

EIRK-01 50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100 each
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Opti-Seal qPCR Roche 480 (Low profile)

G913 100 Plates/Case
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Opti-Seal qPCR Roche 480 (Low profile)

G914 10 Plates/Box
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SanPrep Column microRNA Miniprep Kit

SK8811 50prep
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Total Exosome RNA Extraction Kit (Biological Fluids/Culture Media Supplement)

EIRK-03 20 reactions
EUR 3380.4
Description: This product is a useful tool for the isolation and efficient total RNA (miRNA and mRNAs) extraction from the total exosome population from human biofluids or cell culture media.

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100 each
EUR 1161.6

MicroRNA Precursor Pooled Plasmid Library

PMIRHPLPA-1 1 aliquot plasmid DNA
EUR 60

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1032-10 each
EUR 1057.2

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

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TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)

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TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)

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EUR 796.8

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

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TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

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TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)

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TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)

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EUR 796.8

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)

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MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions)

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MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)

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MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 5 reactions)

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EUR 777.6

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)

M1035-10 each
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The expression levels of miRNAs are continually changing over time was observed and analysis of miRNAs function suggests a potential role as a therapeutic biomarker. In addition, a prediction model for KD showed 78.8% sensitivity and 71.4% specificity, respectively. This study uses small RNA sequencing to identify miRNA biomarkers KD diagnosis is based on a large sample size. Our findings illuminate understanding of the molecular pathogenesis of KD and can improve the accuracy of diagnosis and prognosis in clinical KD.