Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles

Background: There is a significant variability in weight loss after bariatric surgery. We hypothesize that part of this variability can be predetermined by the genetic differences associated with metabolic homeostasis. MicroRNA (miRNA) are short pieces of RNA that regulate gene expression and easily detectable in the serum. They are involved in many metabolic processes, including weight homeostasis. In this pilot study, we briefly review the role of miRNA, and assess the feasibility of using them in a clinical setting treatment of obesity.


Objective: To evaluate the feasibility of using miRNA to predict weight loss after bariatric surgery.


Setting: Academic medical center.


Methods: Serum collected from patients at the initial bariatric surgery consultation. Weight loss data were collected 6 and 12 months postoperatively. Individuals experiencing a bit and the largest number of percentage of excess weight lost at 6 months were analyzed to assess genetic differences in miRNA expression.


Results: The median percentage of excess weight lost was 51% (range, 34% -63%) for those who lose the least and 87% (range, 82% -111%) for those who lost the most weight. Groups were similar in age, gender, diabetes status, and type of operation. In total, of 119 miRNA is detected in the serum of patients, 6 showed potential to differentiate between weight loss group high and low. Mirna has previously been involved in the regulation of fatty acid biosynthesis, adipocytes proliferation, type 2 diabetes, and obesity.


Conclusions: In this pilot study, we demonstrate the feasibility of identifying genetic differences between groups of weight loss by identifying high and low serum miRNA different. In the near future, these biomarkers could facilitate decisions about surgery. In addition, this miRNA could open up new genetic pathways that describe the pathophysiology of obesity, and provide targets for future treatment.

Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles
Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles

The platelet microRNA Profile Kawasaki Disease: Identification of Novel Diagnostic Biomarkers

Challenging the unknown diagnosis and etiology of Kawasaki disease (KD) increases the incidence of coronary artery lesions. microRNAs (miRNAs) are the most promising biomarker for their stability in peripheral blood and noninvasive measurement procedures, which have proven potential utility in cancer. To explore the utility expressed differently (DE) miRNAs early diagnostic markers, 44 patients (25 KD 19, KD incomplete and complete) and 31 controls were recruited fever for small RNA sequencing.

Of all the states in 1922 Mirna, DE 210 miRNAs were found between KD and control groups fever. Although platelet miRNA profile of a complete full KD KD similar through cluster analysis, DE miRNAs are not identical. DE eight miRNAs validated by quantitative real-time PCR (qRT-PCR) in a group of complete or incomplete KD using a normalizer, miR-126-3p, identified by geNorm and NormFinder tools.

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

SeraMir Control spike-in Small RNA and qPCR assay

RA805A-1 100 assays
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Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 1056

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
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SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA820TC-1 20 profiles
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Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA821TC-1 20 profiles
EUR 1533

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 1198.8

SeraMir 3'-Universal Primer (10,000 qPCR reactions)

RA800P-3 10,000 reactions
EUR 125

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 1020

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
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Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
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LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
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rno-miRNome MicroRNA Profiling Kit [Rat]

RA680B-1 20 complete profiles
EUR 2006.4

Stem Cell MicroRNA Profiling Panel

RA620A-1 10 profiles
EUR 1406.4

hsa-miRNome MicroRNA Profiling Kit [Human]

RA660A-1 20 profiles
EUR 2212.8

mmu-miRNome MicroRNA Profiling Kit [Mouse]

RA670A-1 20 profiles
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Psoriasis Exosome RNA

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Prostate Cancer Exosome RNA

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Lung Cancer Exosome RNA

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Normal human exosome RNA

P240 -
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Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

Leukemia - AML Exosome RNA

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Leukemia - CLL Exosome RNA

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Leukemia - CML Exosome RNA

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Liver Cancer Exosome RNA

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Breast Cancer Exosome RNA

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Crohn's Disease Exosome RNA

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Kidney Cancer Exosome RNA

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Bladder Cancer Exosome RNA

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Thyroid Cancer Exosome RNA

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Colorectal Cancer Exosome RNA

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Human CD9 Expressed Exosome Detection Assay kit

6036 1 kit
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Description: Culture Media, Plasma, Serum, Purified Samples

Rheumatoid Arthritis Exosome RNA

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Human CD63 Expressed Exosome Detection Assay Kit

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Nasopharyngeal Cancer Exosome RNA

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Diabetes (Type 1) Exosome RNA

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Diabetes (Type 2) Exosome RNA

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Exosome electron microscopy imaging (EM)

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Human CD9xCD63 Expressed Exosome Detection Assay Kit

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Description: Culture Media, Serum, Plasma

Exosome Component 2 (EXOSC2) Magnetic Luminex Assay Kit

LKU603065-96T 96T
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Exosome Component 7 (EXOSC7) Magnetic Luminex Assay Kit

LKU603072-96T 96T
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LiqBiopsy Tumor Exosome RNA Kit

K1254-24 each
EUR 1332

Leukemia - Hairy Cell Exosome RNA

P241-HC - Ask for price

Melanoma (skin cancer) Exosome RNA

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Uterine Corpus Cancer Exosome RNA

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Non-Hodgkin lymphoma Exosome RNA

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Uterine Cervical Cancer Exosome RNA

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ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)

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EUR 888

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EVery miRNome Profiler for Human Serum and Plasma

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Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

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Systemic Lupus Erythematosus Exosome RNA

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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

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Exosome RNA Helicase MTR4 (MTR4) Antibody

abx149899-100ug 100 ug
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TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

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TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

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GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

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ExoQuick Exosome RNA Column Purification kit

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ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)

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ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)

M1014-100-W each
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ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)

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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-051L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)

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Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

20-abx004268
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  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
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  • 50 ul

Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

abx004268-100l 100 µl
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Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

abx004268-20l 20 µl
EUR 175

Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

abx004268-50l 50 µl
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ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

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ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

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ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

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ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

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Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

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Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

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Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

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ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay)

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EUR 1136.4

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, luminometric assay)

M1005-100-W each
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ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, fluorometric assay)

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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)

CEIK-094L-10T 10T Ask for price

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CEIK-094L-20T 20T Ask for price

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)

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ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)

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ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 5 reactions)

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

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EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100 each
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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100 each
EUR 1161.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1030-10 each
EUR 1005.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1030-20 each
EUR 1546.8

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1030-3 each
EUR 529.2

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)

M1030-5 each
EUR 796.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1031-10 each
EUR 1005.6

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1031-20 each
EUR 1546.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1031-3 each
EUR 523.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)

M1031-5 each
EUR 796.8

Opti-Seal qPCR Roche 480 (Low profile)

G913 100 Plates/Case
EUR 362.4

Opti-Seal qPCR Roche 480 (Low profile)

G914 10 Plates/Box
EUR 91.2

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100 each
EUR 1161.6

Exosome (Pre-isolated) RNA Extraction kit Note! This kit doesn't contain the conponents for exosome enrichment.

EIRK-01 50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

Total Exosome RNA Extraction Kit (Biological Fluids/Culture Media Supplement)

EIRK-03 20 reactions
EUR 3380.4
Description: This product is a useful tool for the isolation and efficient total RNA (miRNA and mRNAs) extraction from the total exosome population from human biofluids or cell culture media.

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1032-10 each
EUR 1057.2

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

M1032-20 each
EUR 1618.8

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)

M1032-3 each
EUR 523.2

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)

M1032-5 each
EUR 796.8

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1033-10 each
EUR 1057.2

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

M1033-20 each
EUR 1618.8

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)

M1033-3 each
EUR 523.2

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)

M1033-5 each
EUR 796.8

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)

M1034-10 each
EUR 1005.6

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions)

M1034-20 each
EUR 1495.2

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)

M1034-3 each
EUR 398.4

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 5 reactions)

M1034-5 each
EUR 777.6

The expression levels of miRNAs are continually changing over time was observed and analysis of miRNAs function suggests a potential role as a therapeutic biomarker. In addition, a prediction model for KD showed 78.8% sensitivity and 71.4% specificity, respectively. This study uses small RNA sequencing to identify miRNA biomarkers KD diagnosis is based on a large sample size. Our findings illuminate understanding of the molecular pathogenesis of KD and can improve the accuracy of diagnosis and prognosis in clinical KD.