Background: There is a significant variability in weight loss after bariatric surgery. We hypothesize that part of this variability can be predetermined by the genetic differences associated with metabolic homeostasis. MicroRNA (miRNA) are short pieces of RNA that regulate gene expression and easily detectable in the serum. They are involved in many metabolic processes, including weight homeostasis. In this pilot study, we briefly review the role of miRNA, and assess the feasibility of using them in a clinical setting treatment of obesity.
Objective : To evaluate the feasibility of using miRNA to predict weight loss after bariatric surgery.
Setting : Academic medical center.
Methods: Serum collected from patients at the initial bariatric surgery consultation. Weight loss data were collected 6 and 12 months postoperatively. Individuals experiencing a bit and the largest number of percentage of excess weight lost at 6 months were analyzed to assess genetic differences in miRNA expression.
Results: The median percentage of excess weight lost was 51% (range, 34% -63%) for those who lose the least and 87% (range, 82% -111%) for those who lost the most weight. Groups were similar in age, gender, diabetes status, and type of operation. In total, of 119 miRNA is detected in the serum of patients, 6 showed potential to differentiate between weight loss group high and low. Mirna has previously been involved in the regulation of fatty acid biosynthesis, adipocytes proliferation, type 2 diabetes, and obesity.
Conclusions : In this pilot study, we demonstrate the feasibility of identifying genetic differences between groups of weight loss by identifying high and low serum miRNA different. In the near future, these biomarkers could facilitate decisions about surgery. In addition, this miRNA could open up new genetic pathways that describe the pathophysiology of obesity, and provide targets for future treatment.
Can genetics help predict efficacy of bariatric surgery? An analysis of microRNA profiles
The platelet microRNA Profile Kawasaki Disease: Identification of Novel Diagnostic Biomarkers
Challenging the unknown diagnosis and etiology of Kawasaki disease (KD) increases the incidence of coronary artery lesions. microRNAs (miRNAs) are the most promising biomarker for their stability in peripheral blood and noninvasive measurement procedures, which have proven potential utility in cancer. To explore the utility expressed differently (DE) miRNAs early diagnostic markers, 44 patients (25 KD 19, KD incomplete and complete) and 31 controls were recruited fever for small RNA sequencing.
Of all the states in 1922 Mirna, DE 210 miRNAs were found between KD and control groups fever. Although platelet miRNA profile of a complete full KD KD similar through cluster analysis, DE miRNAs are not identical. DE eight miRNAs validated by quantitative real-time PCR (qRT-PCR) in a group of complete or incomplete KD using a normalizer, miR-126-3p, identified by geNorm and NormFinder tools.
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1
SBI
20 preps
EUR 493
SeraMir Control spike-in Small RNA and qPCR assay
RA805A-1
SBI
100 assays
EUR 181
Regulatory RNA qPCR Profiler
RA950A-1
SBI
20 rxn
EUR 1056
Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
RA822TC-1
SBI
20 profiles
EUR 1533
SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1
SBI
10 preps
EUR 493
Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)
RA820TC-1
SBI
20 profiles
EUR 1533
Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)
RA821TC-1
SBI
20 profiles
EUR 1533
SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1
SBI
10 reactions
EUR 595
Neural Lineage qPCR Profiler
RA500A-1
SBI
10 reactions
EUR 1198.8
SeraMir 3'-Universal Primer (10,000 qPCR reactions)
RA800P-3
SBI
10,000 reactions
EUR 125
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA900A-1
SBI
20 profiles
EUR 1020
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles
RA930A-1
SBI
20 profiles
EUR 1020
Cancer MicroRNA qPCR Array
RA610A-1
SBI
10 profiles
EUR 1406.4
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
RA910A-1
SBI
20 profiles
EUR 1329.6
rno-miRNome MicroRNA Profiling Kit [Rat]
RA680B-1
SBI
20 complete profiles
EUR 2006.4
Stem Cell MicroRNA Profiling Panel
RA620A-1
SBI
10 profiles
EUR 1406.4
hsa-miRNome MicroRNA Profiling Kit [Human]
RA660A-1
SBI
20 profiles
EUR 2212.8
mmu-miRNome MicroRNA Profiling Kit [Mouse]
RA670A-1
SBI
20 profiles
EUR 2046
Psoriasis Exosome RNA
P241-PS
101Bio
-
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Prostate Cancer Exosome RNA
P241-PR
101Bio
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Lung Cancer Exosome RNA
P241-LG
101Bio
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Normal human exosome RNA
P240
101Bio
-
EUR 1499
Leukemia - ALL Exosome RNA
P241-ALL
101Bio
-
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Leukemia - AML Exosome RNA
P241-AML
101Bio
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Leukemia - CLL Exosome RNA
P241-CLL
101Bio
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Leukemia - CML Exosome RNA
P241-CML
101Bio
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Liver Cancer Exosome RNA
P241-LV
101Bio
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Breast Cancer Exosome RNA
P241-BR
101Bio
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Crohn's Disease Exosome RNA
P241-CR
101Bio
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Kidney Cancer Exosome RNA
P241-KN
101Bio
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Bladder Cancer Exosome RNA
P241-BL
101Bio
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Thyroid Cancer Exosome RNA
P241-TH
101Bio
-
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Colorectal Cancer Exosome RNA
P241-CO
101Bio
-
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Human CD9 Expressed Exosome Detection Assay kit
6036
Chondrex
1 kit
EUR 377
Description: Culture Media, Plasma, Serum, Purified Samples
Rheumatoid Arthritis Exosome RNA
P241-RA
101Bio
-
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Human CD63 Expressed Exosome Detection Assay Kit
6037
Chondrex
1 kit
EUR 377
Description: Culture Media, Plasma, Serum, Purified Samples
Nasopharyngeal Cancer Exosome RNA
P241-NA
101Bio
-
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Diabetes (Type 1) Exosome RNA
P241-DB1
101Bio
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Diabetes (Type 2) Exosome RNA
P241-DB2
101Bio
-
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Exosome electron microscopy imaging (EM)
S109
101Bio
-
EUR 600
Human CD9xCD63 Expressed Exosome Detection Assay Kit
6038
Chondrex
1 kit
EUR 377
Description: Culture Media, Serum, Plasma
Exosome Component 2 (EXOSC2) Magnetic Luminex Assay Kit
LKU603065-96T
Biomatik Corporation
96T
EUR 966
Exosome Component 7 (EXOSC7) Magnetic Luminex Assay Kit
LKU603072-96T
Biomatik Corporation
96T
EUR 1062.6
LiqBiopsy Tumor Exosome RNA Kit
K1254-24
Biovision
each
EUR 1332
Leukemia - Hairy Cell Exosome RNA
P241-HC
101Bio
-
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Melanoma (skin cancer) Exosome RNA
P241-MN
101Bio
-
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Uterine Corpus Cancer Exosome RNA
P241-UT
101Bio
-
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Non-Hodgkin lymphoma Exosome RNA
P241-NH
101Bio
-
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Uterine Cervical Cancer Exosome RNA
P241-UC
101Bio
-
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ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)
M1007-100-T
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, serum, luminometric assay)
M1008-100-W
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, serum, fluorimetric assay)
M1009-100-B
Biovision
each
EUR 888
Leukemia - Multiple Myeloma Exosome RNA
P241-MM
101Bio
-
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EVery miRNome Profiler for Human Serum and Plasma
EVery500B-1
SBI
8 profiles
EUR 1018
Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)
P101NGS
101Bio
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Systemic Lupus Erythematosus Exosome RNA
P241-SL
101Bio
-
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-50T
Creative Bioarray
50T
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Exosome RNA Helicase MTR4 (MTR4) Antibody
abx149899-100ug
Abbexa
100 ug
EUR 526.8
TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1016-100-T
Biovision
each
EUR 874.8
TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1017-100-W
Biovision
each
EUR 888
TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1018-100-B
Biovision
each
EUR 888
GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1022-100-T
Biovision
each
EUR 888
GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1023-100-W
Biovision
each
EUR 888
GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1024-100-B
Biovision
each
EUR 888
ExoQuick Exosome RNA Column Purification kit
EQ808A-1
SBI
20 preps
EUR 344
NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1019-100-T
Biovision
each
EUR 907.2
NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1020-100-W
Biovision
each
EUR 888
NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1021-100-B
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)
M1010-100-T
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)
M1011-100-W
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)
M1012-100-B
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)
M1013-100-T
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)
M1014-100-W
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)
M1015-100-B
Biovision
each
EUR 888
ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-50T
Creative Bioarray
50T
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Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody
20-abx004268
Abbexa
Ask for price
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100 ul
200 ul
20 ul
50 ul
Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody
abx004268-100l
Abbexa
100 µl
EUR 400
Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody
abx004268-20l
Abbexa
20 µl
EUR 175
Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody
abx004268-50l
Abbexa
50 µl
EUR 275
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-10
Biovision
each
EUR 1005.6
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-20
Biovision
each
EUR 1546.8
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-3
Biovision
each
EUR 523.2
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-5
Biovision
each
EUR 796.8
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)
K1203-100
Biovision
each
EUR 1161.6
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)
K1208-100
Biovision
each
EUR 1161.6
Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1025-100-T
Biovision
each
EUR 888
Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1026-100-W
Biovision
each
EUR 888
Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1027-100-B
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay)
M1004-100-T
Biovision
each
EUR 1136.4
ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, luminometric assay)
M1005-100-W
Biovision
each
EUR 888
ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, fluorometric assay)
M1006-100-B
Biovision
each
EUR 888
ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-10T
Creative Bioarray
10T
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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-20T
Creative Bioarray
20T
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ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)
M1039-10
Biovision
each
EUR 1005.6
ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions)
M1039-20
Biovision
each
EUR 1546.8
ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)
M1039-3
Biovision
each
EUR 523.2
ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 5 reactions)
M1039-5
Biovision
each
EUR 796.8
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)
K1205-100
Biovision
each
EUR 1161.6
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)
K1210-100
Biovision
each
EUR 1161.6
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)
K1202-100
Biovision
each
EUR 1161.6
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)
K1206-100
Biovision
each
EUR 1161.6
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)
K1207-100
Biovision
each
EUR 1161.6
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)
M1030-10
Biovision
each
EUR 1005.6
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)
M1030-20
Biovision
each
EUR 1546.8
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)
M1030-3
Biovision
each
EUR 529.2
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)
M1030-5
Biovision
each
EUR 796.8
ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)
M1031-10
Biovision
each
EUR 1005.6
ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)
M1031-20
Biovision
each
EUR 1546.8
ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)
M1031-3
Biovision
each
EUR 523.2
ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)
M1031-5
Biovision
each
EUR 796.8
Opti-Seal qPCR Roche 480 (Low profile)
G913
ABM
100 Plates/Case
EUR 362.4
Opti-Seal qPCR Roche 480 (Low profile)
G914
ABM
10 Plates/Box
EUR 91.2
ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)
K1201-100
Biovision
each
EUR 1161.6
Exosome (Pre-isolated) RNA Extraction kit
Note! This kit doesn't contain the conponents for exosome enrichment.
EIRK-01
Creative Biolabs
50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.
ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1
SBI
20 preps
EUR 493
Total Exosome RNA Extraction Kit (Biological Fluids/Culture Media Supplement)
EIRK-03
Creative Biolabs
20 reactions
EUR 3380.4
Description: This product is a useful tool for the isolation and efficient total RNA (miRNA and mRNAs) extraction from the total exosome population from human biofluids or cell culture media.
TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)
M1032-10
Biovision
each
EUR 1057.2
TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)
M1032-20
Biovision
each
EUR 1618.8
TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)
M1032-3
Biovision
each
EUR 523.2
TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)
M1032-5
Biovision
each
EUR 796.8
TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)
M1033-10
Biovision
each
EUR 1057.2
TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)
M1033-20
Biovision
each
EUR 1618.8
TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)
M1033-3
Biovision
each
EUR 523.2
TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 5 reactions)
M1033-5
Biovision
each
EUR 796.8
MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)
M1034-10
Biovision
each
EUR 1005.6
MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions)
M1034-20
Biovision
each
EUR 1495.2
MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)
M1034-3
Biovision
each
EUR 398.4
MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 5 reactions)
M1034-5
Biovision
each
EUR 777.6
The expression levels of miRNAs are continually changing over time was observed and analysis of miRNAs function suggests a potential role as a therapeutic biomarker. In addition, a prediction model for KD showed 78.8% sensitivity and 71.4% specificity, respectively. This study uses small RNA sequencing to identify miRNA biomarkers KD diagnosis is based on a large sample size. Our findings illuminate understanding of the molecular pathogenesis of KD and can improve the accuracy of diagnosis and prognosis in clinical KD.