Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy

Tang-luo-ning (TLN) is a traditional Chinese herbal recipe that has been used to treat diabetic peripheral neuropathy (DPN); However, the underlying mechanisms are still unclear. This study aims to investigate microRNA (miRNA) expression profiles in diabetic rats treated with TLN, and the predicted target genes. Male Sprague-Dawley rats were randomly divided into control, diabetes, and TLN-treated diabetic groups. Diabetes was induced with streptozotocin, and TLN (5 g / kg / day) orally administered for eight weeks.

Then, the sciatic nerve is harvested for miRNA microarray analysis. The differentially expressed miRNAs and their target genes analyzed. Compared with control mice, 24 miRNAs were significantly upregulated and 59 were downregulated in the sciatic nerves of diabetic mice with more than two-fold (all P <0.05). On TLN-treated diabetic mice, 26 miRNAs were upregulated and 14 downregulated compared with diabetic mice without treatment TLN (all P <0.05). DPN-induced changes of miRNA profiles TLN reversed with treatment.

A total of 1,402 of the target genes screened. In the GO analysis, gene localization, cytoplasm, and the enriched protein binding, and the most significantly enriched including neurotrophin, Fc epsilon RI, and the Wnt signaling pathway. Further analysis revealed that DVL1 and NTF3 genes involved in this pathway. Our findings suggest that TLN can affect Wnt and neurotrophin pathway by acting on the genes DVL1 and NTF3.

 Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy
Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy

Find signatures of lupus-related microRNA profile in the Gene Expression Omnibus repository

Lupus is one of the most common autoimmune disease systemic. It is a multifactorial disease that is genetic, epigenetic and environment plays a significant role. The pathogenesis of lupus is not well understood. However, deregulation of microRNAs (miRNAs) – one of the post-transcriptional regulators of gene – may contribute to the development of autoimmune disease.

Over the past two decades, advances in miRNA profiling using microarray has received a lot of attention, and it has been demonstrated that miRNAs play a regulatory role in the pathogenesis of lupus. Therefore, dysregulated miRNAs can be considered as a promising diagnostic biomarker for lupus. This article is an overview of lupus-related miRNA array profiles and studies in the Gene Expression Omnibus (GEO) database. The aim of our research is to expand the current knowledge dysregulated miRNAs known as a potential biomarker of SLE and to introduce a bioinformatics approach to using microarray data and find novel miRNA and gene candidates for further study. We identified HSA-miR-4709-5p, HSA-mir-140, mir-145-HSA, HSA-mir-659, mir-134-HSA, HSA-mir-150, mir-584-HSA, HSA-Mirza HSA-409 and miR-152 as a potential biomarker with integrated bioinformatics analysis.

Microsome Isolation Kit

K249-50
EUR 528

Preadipocyte Isolation Kit

K583-5
EUR 533

Total RNA Isolation Kit

P-9100 50 samples
EUR 347.25
Description: The best epigenetics products

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 415
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 258

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 258

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 599

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 321

ExoPure? Isolation Kit   (Urine)

K1240-10
EUR 697

ExoPure? Isolation Kit   (Urine)

K1240-2
EUR 403

Apoptotic Cell Isolation Kit

K258-30
EUR 468

Yeast Mitochondria Isolation Kit

K259-50
EUR 419

Mitochondrial DNA Isolation Kit

K280-50
EUR 588

Genomic DNA Isolation Kit

K281-50
EUR 272

Yeast Nuclei Isolation Kit

K289-50
EUR 392

Lipopolysaccharide (LPS) Isolation Kit

K438-10
EUR 278

Apoptotic Cell Isolation Kit

55R-1349 30 units
EUR 729
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 825
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 385
Description: Genomic DNA Isolation Kit for use in the research laboratory

Cartilage RNA Isolation Kit

K2031010 1 kit
EUR 419
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 256

Urine DNA Isolation Kit

K5011150 1 kit
EUR 256

Stool DNA Isolation Kit

K5011250 1 kit
EUR 256

Serum DNA Isolation Kit

K5018100 1 kit
EUR 360

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Circulating Nuleic Acid Isolation Kit

FACFK-050 50 preps
EUR 491

Circulating Nuleic Acid Isolation Kit

FACFK-100 100 preps
EUR 825

Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 308

Total RNA Isolation Kit II

FATRS--050 50 preps
EUR 216

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 79.69

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 81.97

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 164.19

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 506.75

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 270.4
Description: The best epigenetics products

FitAmp Urine DNA Isolation Kit

P-1017 100 Samples
EUR 395.1
Description: kits suitable for this type of research

ChromaFlash Chromatin Isolation & Shearing Kit

P-2023 100 Preperations
EUR 270.4
Description: kits suitable for this type of research

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 347.25
Description: reagents widely cited

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 347.25
Description: kits suitable for this type of research

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 79.69

Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-500P 500 Preps Ask for price

Easy3-Total NucleicAcid Isolation Kit

FYG504-100ML 100 ml Ask for price

Easy3-Total NucleicAcid Isolation Kit

FYG504-500ML 500 ml Ask for price

Exosome Isolation kit (for urine)

P120 - Ask for price

Exosome Isolation kit (for urine)

P120L - Ask for price

Exosome Isolation kit (for urine)

P120S - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-2 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-20 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-80 - Ask for price

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Mouse Brain Microvessel Isolation Kit

P750-4 - Ask for price

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 502

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 551

ExoPure? Isolation Kit   (Cell Media)

K1237-10
EUR 664

ExoPure? Isolation Kit   (Cell Media)

K1237-2
EUR 392

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10
EUR 697

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2
EUR 392

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10
EUR 697

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2
EUR 403

Apoptotic DNA Ladder Isolation Kit

K170-50
EUR 468

EZExtract? Core Histone Isolation Kit

K234-100
EUR 262

Cell Surface Protein Isolation Kit

K295-10
EUR 501

Bacterial Genomic DNA Isolation Kit

K309-100
EUR 376

OrgFrontier? Plasma Membrane Isolation Kit

K414-10
EUR 566

Org Frontier? Chloroplast Isolation Kit

K468-10
EUR 555

Whole Blood DNA Isolation Kit

K528-100
EUR 376

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 386

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PSEUDOMONAS ISOLATION AGAR

P16-114-10kg 10 kg
EUR 1715

PSEUDOMONAS ISOLATION AGAR

P16-114-2kg 2kg
EUR 412

PSEUDOMONAS ISOLATION AGAR

P16-114-500g 500 g
EUR 148

PSEUDOMONAS ISOLATION BROTH

P16-115-10kg 10 kg Ask for price

PSEUDOMONAS ISOLATION BROTH

P16-115-2Kg 2 Kg Ask for price

PSEUDOMONAS ISOLATION BROTH

P16-115-500g 500 g Ask for price

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 158

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 201

Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 216

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 150

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 187

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 76.64

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 76.64

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 395.1
Description: The best epigenetics products

FitAmp Plasma/Serum DNA Isolation Kit

P-1004 100 Samples
EUR 347.25
Description: fast delivery possible

Mammalian Mitochondria Isolation Kit for Tissue

abx098857-50rxns 50 rxns
EUR 411

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Exosome Isolation kit (for serum & plasma)

P101 - Ask for price

Exosome Isolation kit (for serum & plasma)

P101L - Ask for price

Exosome Isolation kit (for serum & plasma)

P101S - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121 - Ask for price


The diagnosis of obstructive sleep apnea (OSA) in Alzheimer’s disease (AD) by polysomnography (PSG) challenging because of the collaboration required of the patient. In addition, screening questionnaires have shown limited usefulness in this subpopulation. Considering this, we investigated circulating microRNA (miRNA) profiles associated with OSA in patients with AD. This study included a carefully selected group of females with mild-moderate AD is confirmed by biological evaluation (n = 29). Individuals submitted to one night PSG to diagnose (apnea-hypopnea index ≥ 15 / h) OSA and the blood collected in the next morning.