Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy
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Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy
Tang-luo-ning (TLN) is a traditional Chinese herbal recipe that has been used to treat diabetic peripheral neuropathy (DPN); However, the underlying mechanisms are still unclear. This study aims to investigate microRNA (miRNA) expression profiles in diabetic rats treated with TLN, and the predicted target genes. Male Sprague-Dawley rats were randomly divided into control, diabetes, and TLN-treated diabetic groups. Diabetes was induced with streptozotocin, and TLN (5 g / kg / day) orally administered for eight weeks.
Then, the sciatic nerve is harvested for miRNA microarray analysis. The differentially expressed miRNAs and their target genes analyzed. Compared with control mice, 24 miRNAs were significantly upregulated and 59 were downregulated in the sciatic nerves of diabetic mice with more than two-fold (all P <0.05). On TLN-treated diabetic mice, 26 miRNAs were upregulated and 14 downregulated compared with diabetic mice without treatment TLN (all P <0.05). DPN-induced changes of miRNA profiles TLN reversed with treatment.
A total of 1,402 of the target genes screened. In the GO analysis, gene localization, cytoplasm, and the enriched protein binding, and the most significantly enriched including neurotrophin, Fc epsilon RI, and the Wnt signaling pathway. Further analysis revealed that DVL1 and NTF3 genes involved in this pathway. Our findings suggest that TLN can affect Wnt and neurotrophin pathway by acting on the genes DVL1 and NTF3.
Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy
Find signatures of lupus-related microRNA profile in the Gene Expression Omnibus repository
Lupus is one of the most common autoimmune disease systemic. It is a multifactorial disease that is genetic, epigenetic and environment plays a significant role. The pathogenesis of lupus is not well understood. However, deregulation of microRNAs (miRNAs) – one of the post-transcriptional regulators of gene – may contribute to the development of autoimmune disease.
Over the past two decades, advances in miRNA profiling using microarray has received a lot of attention, and it has been demonstrated that miRNAs play a regulatory role in the pathogenesis of lupus. Therefore, dysregulated miRNAs can be considered as a promising diagnostic biomarker for lupus. This article is an overview of lupus-related miRNA array profiles and studies in the Gene Expression Omnibus (GEO) database. The aim of our research is to expand the current knowledge dysregulated miRNAs known as a potential biomarker of SLE and to introduce a bioinformatics approach to using microarray data and find novel miRNA and gene candidates for further study. We identified HSA-miR-4709-5p, HSA-mir-140, mir-145-HSA, HSA-mir-659, mir-134-HSA, HSA-mir-150, mir-584-HSA, HSA-Mirza HSA-409 and miR-152 as a potential biomarker with integrated bioinformatics analysis.
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.
Description: This buffer is intended for use with mammalian cells during magnetic cell isolation or single cell processing. This buffer is optimized and formulated to reduce nonspecific binding and cell clumping
The diagnosis of obstructive sleep apnea (OSA) in Alzheimer’s disease (AD) by polysomnography (PSG) challenging because of the collaboration required of the patient. In addition, screening questionnaires have shown limited usefulness in this subpopulation. Considering this, we investigated circulating microRNA (miRNA) profiles associated with OSA in patients with AD. This study included a carefully selected group of females with mild-moderate AD is confirmed by biological evaluation (n = 29). Individuals submitted to one night PSG to diagnose (apnea-hypopnea index ≥ 15 / h) OSA and the blood collected in the next morning.
