Expression profile of microRNAs in the testes of patients with Klinefelter syndrome

Klinefelter syndrome (KS) is the most common sex chromosome aneuploidy. Typical characteristics of KS is oligozoospermia. Although several studies have described the natural history of the degenerative process germ cells in patients with KS, the molecular mechanisms that initiate this process is not well marked.

MicroRNA (miRNA) -mediated post-transcriptional control mechanisms has been increasingly recognized as an important regulator of spermatogenesis; However, only a few studies have evaluated the role of miRNAs in gonadal failure patients.

Here, we describe the differential expression profiles of miRNAs in samples of testicular tissue taken from patients with KS. We analyzed samples of testicular tissue from 4 KS patients and 5 control patients (obstructive azoospermia) through generation sequencing, which can provide information about the mechanisms involved in the degeneration of germ cells. Typical differential expression profiles for 166 miRNAs identified in KS patients: 66 were upregulated and 100 downregulated.

A interactome analysis conducted for 7 of the 20 miRNAs upregulated and downregulated. The results showed that the target gene is involved in the development process, proliferation, and differentiation of spermatogenesis, which may explain their role in the development of infertility. This is the first report of a miRNA expression profiles generated from samples of testicular tissue KS patients.

 Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome

MicroRNA profiles altered in neonatal pigs following the milk glands after giving birth to a nursing infant formula

Postnatal dietary modulation of microRNAs (miRNAs) and the effect on miRNA-mRNA interactions in tissues remains unknown. This study aimed to investigate whether dietary factors (formula vs. breastfeeding) affected milk miRNA expression and to determine whether these changes coincide with changes in mammary gland development in neonatal piglets. Female Yorkshire / Duroc pig pigs fed milk or milk- or soy-based infant formula milk cow (from postnatal day 2 days 21; n = 6 / group).

Different miRNAs expressed milk is determined by using miRNA profiles, followed by miRNA and mRNA expression characterized by reverse-transcription quantitative polymerase chain reaction. Milk and soy formulas reduce the expression of miR-1, -128, -133a, -193b, -206 and -27a; Mirna modified mRNA down-regulation of gene expression (eg, Ccnd1, Tgfb3, Igf1r and Tbx3) consistent with increased cell proliferation and reduced apoptosis in the developing mammary gland.

Interestingly, down-regulation of miR-1, -128 and -27a also correlates with increased gene mRNA encoding such Hmgcr Hmgcs and cholesterol synthesis in the mammary gland in response to lower levels of cholesterol circulating. feeding formula milk affected miRNA profile in neonatal pigs, along with increased cell proliferation and gene expression of cholesterol synthesis, indicating the beginning of the nutritional modulation of miRNAs can contribute to the regulation of cholesterol homeostasis status and the proliferation of mammary glands develop during infancy.

microRNA-21-IN-1

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Description: microRNA-21-IN-1

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Description: microRNA-21-IN-1

microRNA-21-IN-1

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Description: microRNA-21-IN-1

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Description: microRNA-21-IN-1

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microRNA-21-IN-2

HY-151516 10 mg
EUR 346.33
Description: microRNA-21-IN-2 is a potential miR-21 inhibitor with an AC50 value of 3.29 μM. microRNA-21-IN-2 can be used for the research of cancer[1].

microRNA-21-IN-1

HY-146411 1 mg
EUR 2943.77
Description: microRNA-21-IN-1 (compound 7A) is an efficient microRNA inhibitor. microRNA-21-IN-1 has antiproliferative activity against Hela and HCT-116 cells with IC50s of 5.5 μM and 2.8 μM respectively, as well as promotes apoptosis of Hela cells. microRNA-21-IN-1 upregulates the expression of microRNA-21 downstream functional targets (PTEN, EGR1 and SLIT2). microRNA-21-IN-1 can be used for researching anticancer[1].

microRNA-21-IN-3

HY-153534 1 mg
EUR 2056.31
Description: microRNA-21-IN-3 (compound 45) can specifically bind to the precursor of oncogenic and pro-inflammatory microRNA-21 with medium nanomolar affinity, reduce cancer cell proliferation and miR-21 levels, and can be used in antitumor research[1].

pGreenPuro Scramble Hairpin Control - Virus (for shRNAs and miRZips)

MZIP000-VA-1 >1 x 10^6 IFUs
EUR 652

MicroRNA Isolation Kit

KS341025 1 kit
EUR 284

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1406.4

Human microRNA-210(miR-210)ELISA

QY-E05678 96T
EUR 433.2

Plant MicroRNA Purification Kit

10760135-1 25 Prep(s)
EUR 245.48

Human MicroRNA-210 (miR-210) ELISA Kit

MBS3800168-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human MicroRNA-210 (miR-210) ELISA Kit

MBS3800168-48StripWells 48-Strip-Wells
EUR 550

Human MicroRNA-210 (miR-210) ELISA Kit

MBS3800168-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human MicroRNA-210 (miR-210) ELISA Kit

MBS3800168-96StripWells 96-Strip-Wells
EUR 765

Human microrna-21(mir-21) ELISA Kit

SL3405Hu 96 Tests
EUR 468

Stem Cell MicroRNA Profiling Panel

RA620A-1 10 profiles
EUR 1406.4

SanPrep Column microRNA Miniprep Kit

SK8811 50prep
EUR 151.32

MicroRNA Precursor Pooled Plasmid Library

PMIRHPLPA-1 1 aliquot plasmid DNA
EUR 60

Expression Plasmid of Rat microRNA MIR1

SC403107 10 µg Ask for price

Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)

PMIRH000PA-1 Bacterial Streak
EUR 594

Expression Plasmid of Rat microRNA MIR16

SC403169 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR21

SC403219 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR22

SC403234 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR25

SC403243 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR28

SC403248 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR32

SC403286 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR33

SC403296 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR93

SC403522 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR96

SC403524 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR98

SC403525 10 µg Ask for price

rno-miRNome MicroRNA Profiling Kit [Rat]

RA680B-1 20 complete profiles
EUR 2006.4

Expression Plasmid of Rat microRNA MIR100

SC403108 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR105

SC403113 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR107

SC403115 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR10a

SC403116 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR10b

SC403117 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR122

SC403121 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR127

SC403132 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR132

SC403141 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR134

SC403145 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR136

SC403148 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR137

SC403149 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR139

SC403152 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR140

SC403153 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR141

SC403154 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR142

SC403155 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR143

SC403156 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR144

SC403157 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR145

SC403158 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR147

SC403161 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR150

SC403163 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR151

SC403164 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR152

SC403165 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR153

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Expression Plasmid of Rat microRNA MIR154

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Expression Plasmid of Rat microRNA MIR15b

SC403168 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR183

SC403178 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR184

SC403180 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR185

SC403182 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR186

SC403183 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR187

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Expression Plasmid of Rat microRNA MIR188

SC403185 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR18a

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Expression Plasmid of Rat microRNA MIR192

SC403192 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR193

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Expression Plasmid of Rat microRNA MIR195

SC403196 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR19a

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Expression Plasmid of Rat microRNA MIR201

SC403207 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR202

SC403208 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR204

SC403211 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR205

SC403212 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR206

SC403213 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR207

SC403214 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR20a

SC403217 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR20b

SC403218 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR210

SC403220 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR211

SC403221 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR212

SC403222 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR214

SC403223 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR215

SC403224 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR217

SC403227 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR221

SC403235 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR222

SC403236 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR223

SC403237 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR224

SC403238 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR23a

SC403239 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR23b

SC403240 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR26a

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Expression Plasmid of Rat microRNA MIR26b

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Expression Plasmid of Rat microRNA MIR27a

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Expression Plasmid of Rat microRNA MIR27b

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Expression Plasmid of Rat microRNA MIR290

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Expression Plasmid of Rat microRNA MIR292

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Expression Plasmid of Rat microRNA MIR293

SC403253 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR294

SC403254 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR296

SC403257 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR297

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Expression Plasmid of Rat microRNA MIR298

SC403259 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR29a

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Expression Plasmid of Rat microRNA MIR29c

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Expression Plasmid of Rat microRNA MIR300

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Expression Plasmid of Rat microRNA MIR30a

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Expression Plasmid of Rat microRNA MIR30d

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Expression Plasmid of Rat microRNA MIR30e

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Expression Plasmid of Rat microRNA MIR31a

SC403284 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR31b

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Expression Plasmid of Rat microRNA MIR320

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Expression Plasmid of Rat microRNA MIR322

SC403288 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR323

SC403289 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR324

SC403290 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR325

SC403291 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR326

SC403292 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR329

SC403295 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR330

SC403297 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR331

SC403298 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR336

SC403299 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR337

SC403300 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR338

SC403301 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR339

SC403302 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR341

SC403303 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR342

SC403304 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR343

SC403305 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR345

SC403312 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR346

SC403313 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR349

SC403315 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR34a

SC403316 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR34b

SC403317 10 µg Ask for price

Expression Plasmid of Rat microRNA MIR34c

SC403318 10 µg Ask for price


placenta-derived exosomes play an important role in cellular communication both in the mother and fetus. concentration and their composition is modified in some disorders of pregnancy, such as gestational diabetes mellitus (GDM). Isolation and characterization of placental exosomes from serum, plasma and tissue from patients with GDM have been described previously; However, to the best of our knowledge, to date, no studies are available on placental exosomes isolated from the urine of patients with GDM.