Expression profile of microRNAs in the testes of patients with Klinefelter syndrome

Klinefelter syndrome (KS) is the most common sex chromosome aneuploidy. Typical characteristics of KS is oligozoospermia. Although several studies have described the natural history of the degenerative process germ cells in patients with KS, the molecular mechanisms that initiate this process is not well marked.

MicroRNA (miRNA) -mediated post-transcriptional control mechanisms has been increasingly recognized as an important regulator of spermatogenesis; However, only a few studies have evaluated the role of miRNAs in gonadal failure patients.

Here, we describe the differential expression profiles of miRNAs in samples of testicular tissue taken from patients with KS. We analyzed samples of testicular tissue from 4 KS patients and 5 control patients (obstructive azoospermia) through generation sequencing, which can provide information about the mechanisms involved in the degeneration of germ cells. Typical differential expression profiles for 166 miRNAs identified in KS patients: 66 were upregulated and 100 downregulated.

A interactome analysis conducted for 7 of the 20 miRNAs upregulated and downregulated. The results showed that the target gene is involved in the development process, proliferation, and differentiation of spermatogenesis, which may explain their role in the development of infertility. This is the first report of a miRNA expression profiles generated from samples of testicular tissue KS patients.

 Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome

MicroRNA profiles altered in neonatal pigs following the milk glands after giving birth to a nursing infant formula

Postnatal dietary modulation of microRNAs (miRNAs) and the effect on miRNA-mRNA interactions in tissues remains unknown. This study aimed to investigate whether dietary factors (formula vs. breastfeeding) affected milk miRNA expression and to determine whether these changes coincide with changes in mammary gland development in neonatal piglets. Female Yorkshire / Duroc pig pigs fed milk or milk- or soy-based infant formula milk cow (from postnatal day 2 days 21; n = 6 / group).

Different miRNAs expressed milk is determined by using miRNA profiles, followed by miRNA and mRNA expression characterized by reverse-transcription quantitative polymerase chain reaction. Milk and soy formulas reduce the expression of miR-1, -128, -133a, -193b, -206 and -27a; Mirna modified mRNA down-regulation of gene expression (eg, Ccnd1, Tgfb3, Igf1r and Tbx3) consistent with increased cell proliferation and reduced apoptosis in the developing mammary gland.

Interestingly, down-regulation of miR-1, -128 and -27a also correlates with increased gene mRNA encoding such Hmgcr Hmgcs and cholesterol synthesis in the mammary gland in response to lower levels of cholesterol circulating. feeding formula milk affected miRNA profile in neonatal pigs, along with increased cell proliferation and gene expression of cholesterol synthesis, indicating the beginning of the nutritional modulation of miRNAs can contribute to the regulation of cholesterol homeostasis status and the proliferation of mammary glands develop during infancy.

Pooled Virus Library of all Lenti-miR microRNA Precursor Constructs [4 virus aliquots]

PMIRHPLVA-1 4 virus aliquots
EUR 4979

Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)

PMIRH000PA-1 Bacterial Streak
EUR 656

pGreenPuro Scramble Hairpin Control - Construct (for shRNAs and miRZips)

MZIP000-AA-1 Miniprep DNA
EUR 684

pGreenPuro Scramble Hairpin Control - Construct (for shRNAs and miRZips)

MZIP000-PA-1 Bacterial Streak
EUR 656

MicroRNA Isolation Kit

KS341025 1 kit
EUR 256
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

pGreenFire1-LXRE in SREBP1c (virus)

TR100VA-P >2 x 10^6 IFUs
EUR 786

pGreenFire1-LXRE in ApoA1(virus)

TR104VA-P >2 x 10^6 IFUs
EUR 786

pGreenFire1-LXRE in Cyp7A1(virus)

TR105VA-P >2 x 10^6 IFUs
EUR 786

pGreenFire1-LXRE in ABCA1(virus)

TR106VA-P >2 x 10^6 IFUs
EUR 786

pGreenFire1-LXRE in ABCG1(virus)

TR107VA-P >2 x 10^6 IFUs
EUR 786

pGreenFire1-LXRE in ApoE(virus)

TR108VA-P >2 x 10^6 IFUs
EUR 786

pGreenFire1-LXRE in CETP(virus)

TR109VA-P >2 x 10^6 IFUs
EUR 786

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172

Virus Measles Virus (MeV) Protein

abx670263-1ml 1 ml
EUR 453

Bovine Leukemia Virus(Leukemia Virus)

QY-E60121 96T
EUR 426

pGreenFire1-LXRE in LXR alpha (virus)

TR102VA-P >2 x 10^6 IFUs
EUR 786

SanPrep Column microRNA Miniprep Kit

SK8811 50prep
EUR 126.1

Stem Cell MicroRNA Profiling Panel

RA620A-1 10 profiles
EUR 1172

MicroRNA Precursor Pooled Plasmid Library

PMIRHPLPA-1 1 aliquot plasmid DNA
EUR 50

Virus Respiratory Syncytial Virus (RSV) Protein

abx670078-01mg 0.1 mg
EUR 495

Virus Hepatitis A Virus (HAV) Protein

abx670259-1ml 1 ml
EUR 356

Virus Varicella Zoster Virus (VZV) Protein

abx670269-1ml 1 ml
EUR 662

Virus West Nile Virus (WNV) Protein

abx670276-100ug 100 ug
EUR 662

Virus Yellow Fever Virus (YFV) Protein

abx670277-100ug 100 ug
EUR 537

Mumps Virus

DAG202 1 ml
EUR 698

EBV Virus

G229 10 ml
EUR 735

miRZip-100 anti-miR-100 microRNA construct, for distribution in Taiwan

MZIP100-AA-1 Miniprep DNA
EUR 716

miRZip-150 anti-miR-150 microRNA construct, for distribution in Taiwan

MZIP150-AA-1 Miniprep DNA
EUR 684

miRZip-205 anti-miR-205 microRNA construct, for distribution in Taiwan

MZIP205-AA-1 Miniprep DNA
EUR 684

miRZip-30c anti-miR-30c microRNA construct, for distribution in Taiwan

MZIP30c-AA-1 Miniprep DNA
EUR 684

Human pre-microRNA Expression Construct Lenti-miR-23b in CD511B-1

PMIRH23b-onlyPA-1 Bacterial Streak
EUR 684

Human microRNA-210(miR-210)ELISA

QY-E05678 96T
EUR 361

hsa-miRNome MicroRNA Profiling Kit [Human]

RA660A-1 20 profiles
EUR 1844

mmu-miRNome MicroRNA Profiling Kit [Mouse]

RA670A-1 20 profiles
EUR 1705

rno-miRNome MicroRNA Profiling Kit [Rat]

RA680B-1 20 complete profiles
EUR 1672

MicroRNA precursor pooled library, HT prepackaged

PMIRHPLVAHT-1 10 virus aliquots
EUR 10035

Chicken Virus Enteritis (Virus Enteritis) ELISA Kit

abx052451-96tests 96 tests
EUR 668

Virus Grapevine Virus A (GVA) ELISA Kit

abx055777-96tests 96 tests
EUR 668

Virus Grapevine Virus B (GVB) ELISA Kit

abx055779-96tests 96 tests
EUR 668

Virus Grapevine Virus D (GVD) ELISA Kit

abx055781-96tests 96 tests
EUR 668

Virus Parainfluenza Virus 1 (HPIV-1) Protein

abx670265-1ml 1 ml
EUR 662

Virus Parainfluenza Virus 2 (HPIV-2) Protein

abx670266-1ml 1 ml
EUR 592

Virus Parainfluenza Virus 3 (HPIV-3) Protein

abx670267-1ml 1 ml
EUR 592

Rabbit Anti-Mumps virus (Enders) Virus antiserum

MUMS11-S 0.1 ml
EUR 457

Rabbit Anti-Mumps virus (Enders) Virus antiserum

MUMS11-SB 0.5 ml
EUR 895

Monoclonal Anti-Mumps virus (Enders) Virus IgG

MUMS12-M 100 ul
EUR 408

Avian leukosis virus Rous sarcoma virus PCR kit

PCR-V049-48R 50T
EUR 823

Avian leukosis virus Rous sarcoma virus PCR kit

PCR-V049-96R 100T
EUR 1113

Rat Corona Virus & Sialoda Cryoadenitis Virus PCR kit

PCR-V386-48R 50T
EUR 823

Rat Corona Virus & Sialoda Cryoadenitis Virus PCR kit

PCR-V386-96R 100T
EUR 1113

Virus Herpes Simplex Virus 2 (HSV-2) Protein

abx670261-1ml 1 ml
EUR 300

Human pre-microRNA Expression Construct Lenti-miR-150, for distribution in Taiwan

PMIRH150AA-1 Miniprep DNA
EUR 684

Human pre-microRNA Expression Construct Lenti-miR-19a in CD513B-1 MI0000073

PMIRH19a-onlyPA-1 Bacterial Streak
EUR 684

Rabies Virus Glycoprotein

HY-P0285 1mg
EUR 257

Sendai virus protein

DAGA-514 1mg
EUR 554

Marburg Virus Antibody

DMAB-A007 1 mg
EUR 1691

Marburg Virus Antibody

DMAB-A008 1 mg
EUR 1691

alpha virus Antibody

abx021534-1mg 1 mg
EUR 1017

Chikungunya Virus Antibody

abx021574-1mg 1 mg
EUR 1080

Chikungunya Virus Antibody

abx021575-1mg 1 mg
EUR 1080

Chikungunya Virus Antibody

abx021576-1mg 1 mg
EUR 1080

Ebola Virus Antibody

abx021693-1mg 1 mg
EUR 1066

Ebola Virus Antibody

abx021694-1mg 1 mg
EUR 1066

HFRS Virus Antibody

abx021759-1mg 1 mg
EUR 954

HFRS Virus Antibody

abx021760-1mg 1 mg
EUR 954

Marburg Virus Antibody

abx022051-1mg 1 mg
EUR 940

Marburg Virus Antibody

abx022052-1mg 1 mg
EUR 940

Marburg Virus Antibody

abx022053-1mg 1 mg
EUR 940

Marburg Virus Antibody

abx022054-1mg 1 mg
EUR 940

Rabies Virus Antibody

abx022163-1mg 1 mg
EUR 1017

Rabies Virus Antibody

abx022164-1mg 1 mg
EUR 648

Rabies Virus Antibody

abx022165-1mg 1 mg
EUR 648

Rabies Virus Antibody

abx022166-1mg 1 mg
EUR 648

Rabies Virus Antibody

abx022167-1mg 1 mg
EUR 648

Rabies Virus Antibody

abx022169-1mg 1 mg
EUR 648

Rabies Virus Antibody

abx022170-1mg 1 mg
EUR 648

Vaccinia Virus Antibody

abx022301-1mg 1 mg
EUR 1017

Vaccinia Virus Antibody

abx022303-1mg 1 mg
EUR 1017

Vaccinia Virus Antibody

abx022304-1mg 1 mg
EUR 1121

Rubella Virus Antibody

abx023129-1ml 1 ml
EUR 537

Ebola Virus Antibody

abx023559-1mg 1 mg
EUR 996

Measles Virus Antibody

abx023654-1mg 1 mg
EUR 878

Measles Virus Antibody

abx023655-1mg 1 mg
EUR 878

Measles Virus Antibody

abx023656-1mg 1 mg
EUR 878

Measles Virus Antibody

abx023657-1mg 1 mg
EUR 878

Dengue Virus Antibody

abx413942-025mg 0.25 mg
EUR 565

Dengue Virus Antibody

abx415691-01mg 0.1 mg
EUR 648

Mumps Virus NucleoProtein

20-abx260298
  • EUR 885.00
  • EUR 342.00
  • EUR 1609.00
  • 0.5 mg
  • 100 ug
  • 1 mg

Mumps Virus Protein

abx670264-1ml 1 ml
EUR 453

Rubella Virus Protein

abx670270-1mg 1 mg
EUR 1010

Rubella Virus Protein

abx670271-1mg 1 mg
EUR 1803

Cytomegalo Virus Mosaic

PR27123 500 ug
EUR 833


placenta-derived exosomes play an important role in cellular communication both in the mother and fetus. concentration and their composition is modified in some disorders of pregnancy, such as gestational diabetes mellitus (GDM). Isolation and characterization of placental exosomes from serum, plasma and tissue from patients with GDM have been described previously; However, to the best of our knowledge, to date, no studies are available on placental exosomes isolated from the urine of patients with GDM.