Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans

The microarnas (mirnas) are small non-coding non-coding RNA fragments that govern the expression of genes and silence at the post-transcriptional level. MIRNAS each controls downstream goals and play roles established in different biological processes. Given that Mirnas has recently been proposed to contribute to the molecular control of sleep sleep regulation in animal models and narcoleptic patients, we investigated the impact of acute sleep deprivation on the expression of Mirna Bloic.

Healthy adult men of two different age groups. Twenty-two young people (average age: 24 ± 3 years) and nine elderly people (65 ± 1 years) have completed a study in controlled laboratory, composed of 8 hours of reference sleep, followed by 40 hours of prolonged awakening and a 10 hour recovery sleep opportunity. At the same time circadian in the three conditions (at 4:23 ± 23 min), the qpcr expression profile of 86 mi mirnas was carried out in the blood serum. Thirteen different mirnas could be quantified reliably and have been analyzed using mixed-model Anovas.

It has been found that MIR-30C and MIR-127 have been reliably affected by sleep and earlier, so that the expression of these mirnas has been regulated after prolonged and standardized expression after recovery sleep. With the previous conclusions of Narcolepsy patients, our preliminary data indicate that MIR-30C and its target proteins can provide a high sleep debt biomarker in humans. Microna (Mirna) plays a key role in antivirus host interactions. Here we used a deep sequencing technology to determine the expression profiles of cellular Mije in chicken dendritic cells infected with the H9N2 Avian Influenza (AIV) virus. A total of 66 new Known Mirnas and 36 new mirnas were expressed differently on H9N2 infection, including 72 mirynas regulated for subsequent regulation and upwards.

Comparative analysis of microarna profiles and sperm mRNA with different freezing tolerance capabilities in the wild boar (Susscrofa) and giant panda (Ailugodamelanoluca).

The quality parameters of post-thawed sperm vary from different species after cryopreservation. To date, the molecular mechanism of the cum of sperm, gel tolerance and other influential factors is largely unknown. In this study, micrarnas (mirnas) and mirnas (MIRNAS) significantly in the boar and giant panda sperm with a different cryo-resistance capability have been evaluated. From the result of a mirna profile of freshly thawed and thawed giant panda sperm, a total of 899 mature mature, new mirnas have been identified and 284 miRNas have been considered significantly regulated (195 regulated up to height and 89). The combined analysis of the mirna profiling of giant panda sperm and our previously published data on sperm, 46, 21 and 4 expressed differentially (de) Armas in the BRAR sperm were related to apoptosis, with glycolysis and oxidative phosphorylation, respectively.

Meanwhile, MRNAS 87, 17 and 7 in giant panda were associated with apoptosis, glycolysis and oxidizing phosphorylation, respectively. The analysis of the ontology of genes (GO) of the Mirnas’s objectives showed that they were mainly distributed on the way of membrane membrane in giant panda sperm, while cellular components and processes Cellular were linked to Mirnas’s targets in wild boar sperm. Finally, the Kyoto’s Encyclopedia of Genes and Genomes (KEGG) Analysis of MRNAS indicated that most of these MRNAS have been distributed in the pathways related to the transduction of membrane signals in the giant panda sperm, while those of the Sperm sperm were mainly distributed in the cytokine-cytokine receptor.

trail of interaction and lanes related to inflammatory. In conclusion, although the different extents and freezing programs have been used, the MIRNAS and the MRNAs involved in apoptosis, energy metabolism, the olfactory transduction path, inflammatory response and cytokine-cytokine interactions, could be The possible molecular mechanism of sperm cryoam and freezing tolerance.

 Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans
Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans

Identification of new microarnas and characterization of microad expression profiles in ovarian follicular zebra follicular cells.

MicroRNAs (MIRNAS) are small non-coding RNAs that govern genes primarily at post-transcriptional levels and play important roles in the regulation of many physiological and development processes. The maturation of oocytes in fish is induced by hormones produced from hypothalamus, pituitary and ovarian. The gonadotropin release hormone (GNRH) stimulates the secretion of luteinizing hormone (LH), which in turn induces the secretion of the hormone inducing the maturation (MIH) of the ovary. It is documented that small follicles first viteelbogens (or stages IIIA) are unable to undergo a maturation of oocytes, while the oocytes of the follicles in the middle or at the end of vitelloges (phase IIIB) can be induced by LH and MIH for Become mature.

To determine if Mirnas can participate in the growth and acquisition of the maturing skill of ovarian follicles, we have determined Mirna’s expression profiles in follicular cells collected from Stage IIIA and IIIB follicles using the help of New generation sequencing. It has been found that mirnas are abundantly expressed in follicular cells of the two stages IIIA and Follicles IIIB. In addition, the analysis of bioinformatics revealed the presence of 214 new new mushas and 44 new mushas in ovarian ovarian follicular cells. Most mature mirnas in follicular cells have been found in the length of 22 nucleotides. The differential expression analysis revealed that MIRNAS were significantly regulated and 13 miRNas were significantly reduced in the Follicular Cells IIIB compared to Follicular Cells. The expression of four of the Mirnas significantly has been validated by real-time PCR.

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T1236227Dia 5 slides
EUR 465
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
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T1236230Dia 5 slides
EUR 465
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Small Intestine Tumor: Duodenum Adenocarcinoma
T2235226-1 5 slides
EUR 257
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Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum
T2236101Dia 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
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T2236227Dia 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum
T2236230Dia 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
OncoMir pooled microRNA precursor virus library
PMIRHOPLVA-1 1 virus aliquot
EUR 1191
  • Category: MicroRNA Tools
MicroRNA precursor pooled library, HT prepackaged
PMIRHPLVAHT-1 10 virus aliquots
EUR 10035
  • Category: MicroRNA Tools
Human microRNA-210(miR-210)ELISA
QY-E05678 96T
EUR 361
hsa-miRNome MicroRNA Profiling Kit [Human]
RA660A-1 20 profiles
EUR 1844
  • Category: MicroRNA Tools
mmu-miRNome MicroRNA Profiling Kit [Mouse]
RA670A-1 20 profiles
EUR 1705
  • Category: MicroRNA Tools
rno-miRNome MicroRNA Profiling Kit [Rat]
RA680B-1 20 complete profiles
EUR 1672
  • Category: MicroRNA Tools
Human Intestine FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)
7-15065 1 Kit Ask for price
Human Intestine FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)
7-15066 1 Kit Ask for price
Human Intestine FibrOut3: Fibroblast Inhibitory System (for 500 ml medium)
7-15067 1 Kit Ask for price
Mouse Intestine FibrOut3: Fibroblast Inhibitory System (for 500 ml medium)
7-15149 1 Kit Ask for price
Mouse Intestine FibrOut4: Fibroblast Inhibitory System (for 500 ml medium)
7-15150 1 Kit Ask for price
Mouse Intestine FibrOut5: Fibroblast Inhibitory System (for 500 ml medium)
7-15151 1 Kit Ask for price
Rat Intestine FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)
7-15233 1 Kit Ask for price
Rat Intestine FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)
7-15234 1 Kit Ask for price
Rat Intestine FibrOut3: Fibroblast Inhibitory System (for 500 ml medium)
7-15235 1 Kit Ask for price
Genomic DNA from Lupus: Small Intestine, from a single donor
D1236226Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Rat Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells Growth Medium
9-25052 5 x 100 ml Ask for price
Rat Intestine PrimaCell2: Normal Intestinal Smooth Muscle Cells Growth Medium
9-25053 5 x 100 ml Ask for price
Rat Intestine PrimaCell3: Normal Itestinal Vein Endothelial Cells Growth Medium
9-25054 5 x 100 ml Ask for price
Mouse Intestine PrimaCell3: Normal Intestinal Mucosa Epithelial Cells Growth Medium
9-32053 5 x 100 ml Ask for price
Mouse Intestine PrimaCell4: Normal Intestinal Smooth Muscle Cells Growth Medium
9-32054 5 x 100 ml Ask for price
Mouse Intestine PrimaCell5: Normal Itestinal Vein Endothelial Cells Growth Medium
9-32055 5 x 100 ml Ask for price
Human Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells Growth Medium
9-46071 5 x 100 ml Ask for price
Human Intestine PrimaCell2: Normal Intestinal Smooth Muscle Cells Growth Medium
9-46072 5 x 100 ml Ask for price
Human Intestine PrimaCell3: Normal Itestinal Vein Endothelial Cells Growth Medium
9-46073 5 x 100 ml Ask for price
Total Protein from Human Adult Normal Tissue: Small Intestine: Duodenum
P1234101 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine: Ileum
P1234227 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine: Jejunum
P1234230 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Duodenum
P3234101 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Ileum
P3234227 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Jejunum
P3234230 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Human Adult Normal Tissue: Small Intestine: Duodenum
R1234101-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine: Ileum
R1234227-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine: Jejunum
R1234230-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum
R1534101-50 50 ug
EUR 316
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum
R1534101-Cy 50 ug
EUR 316
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Finally, the enrichment and gene route analyzes of the predicted targets of the MIRNAS significantly regulated supported the involvement of several key signaling pathways in the regulation of ovarian function, including the maturation of oocytes. Taken together, this study identifies new zebra mirnas and characterizes Mirna’s expression profiles in somatic cells in zebra ovarian follicles. The differential expression of the mirnas between the follicular cells of step IIIA and IIIB suggests that these mushas are important regulatory authorities in the development of the zebrafish ovarian follicle and / or the maturation of oocytes.