Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans
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Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans
The microarnas (mirnas) are small non-coding non-coding RNA fragments that govern the expression of genes and silence at the post-transcriptional level. MIRNAS each controls downstream goals and play roles established in different biological processes. Given that Mirnas has recently been proposed to contribute to the molecular control of sleep sleep regulation in animal models and narcoleptic patients, we investigated the impact of acute sleep deprivation on the expression of Mirna Bloic.
Healthy adult men of two different age groups. Twenty-two young people (average age: 24 ± 3 years) and nine elderly people (65 ± 1 years) have completed a study in controlled laboratory, composed of 8 hours of reference sleep, followed by 40 hours of prolonged awakening and a 10 hour recovery sleep opportunity. At the same time circadian in the three conditions (at 4:23 ± 23 min), the qpcr expression profile of 86 mi mirnas was carried out in the blood serum. Thirteen different mirnas could be quantified reliably and have been analyzed using mixed-model Anovas.
It has been found that MIR-30C and MIR-127 have been reliably affected by sleep and earlier, so that the expression of these mirnas has been regulated after prolonged and standardized expression after recovery sleep. With the previous conclusions of Narcolepsy patients, our preliminary data indicate that MIR-30C and its target proteins can provide a high sleep debt biomarker in humans. Microna (Mirna) plays a key role in antivirus host interactions. Here we used a deep sequencing technology to determine the expression profiles of cellular Mije in chicken dendritic cells infected with the H9N2 Avian Influenza (AIV) virus. A total of 66 new Known Mirnas and 36 new mirnas were expressed differently on H9N2 infection, including 72 mirynas regulated for subsequent regulation and upwards.
Comparative analysis of microarna profiles and sperm mRNA with different freezing tolerance capabilities in the wild boar (Susscrofa) and giant panda (Ailugodamelanoluca).
The quality parameters of post-thawed sperm vary from different species after cryopreservation. To date, the molecular mechanism of the cum of sperm, gel tolerance and other influential factors is largely unknown. In this study, micrarnas (mirnas) and mirnas (MIRNAS) significantly in the boar and giant panda sperm with a different cryo-resistance capability have been evaluated. From the result of a mirna profile of freshly thawed and thawed giant panda sperm, a total of 899 mature mature, new mirnas have been identified and 284 miRNas have been considered significantly regulated (195 regulated up to height and 89). The combined analysis of the mirna profiling of giant panda sperm and our previously published data on sperm, 46, 21 and 4 expressed differentially (de) Armas in the BRAR sperm were related to apoptosis, with glycolysis and oxidative phosphorylation, respectively.
Meanwhile, MRNAS 87, 17 and 7 in giant panda were associated with apoptosis, glycolysis and oxidizing phosphorylation, respectively. The analysis of the ontology of genes (GO) of the Mirnas’s objectives showed that they were mainly distributed on the way of membrane membrane in giant panda sperm, while cellular components and processes Cellular were linked to Mirnas’s targets in wild boar sperm. Finally, the Kyoto’s Encyclopedia of Genes and Genomes (KEGG) Analysis of MRNAS indicated that most of these MRNAS have been distributed in the pathways related to the transduction of membrane signals in the giant panda sperm, while those of the Sperm sperm were mainly distributed in the cytokine-cytokine receptor.
trail of interaction and lanes related to inflammatory. In conclusion, although the different extents and freezing programs have been used, the MIRNAS and the MRNAs involved in apoptosis, energy metabolism, the olfactory transduction path, inflammatory response and cytokine-cytokine interactions, could be The possible molecular mechanism of sperm cryoam and freezing tolerance.
Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans
Identification of new microarnas and characterization of microad expression profiles in ovarian follicular zebra follicular cells.
MicroRNAs (MIRNAS) are small non-coding RNAs that govern genes primarily at post-transcriptional levels and play important roles in the regulation of many physiological and development processes. The maturation of oocytes in fish is induced by hormones produced from hypothalamus, pituitary and ovarian. The gonadotropin release hormone (GNRH) stimulates the secretion of luteinizing hormone (LH), which in turn induces the secretion of the hormone inducing the maturation (MIH) of the ovary. It is documented that small follicles first viteelbogens (or stages IIIA) are unable to undergo a maturation of oocytes, while the oocytes of the follicles in the middle or at the end of vitelloges (phase IIIB) can be induced by LH and MIH for Become mature.
To determine if Mirnas can participate in the growth and acquisition of the maturing skill of ovarian follicles, we have determined Mirna’s expression profiles in follicular cells collected from Stage IIIA and IIIB follicles using the help of New generation sequencing. It has been found that mirnas are abundantly expressed in follicular cells of the two stages IIIA and Follicles IIIB. In addition, the analysis of bioinformatics revealed the presence of 214 new new mushas and 44 new mushas in ovarian ovarian follicular cells. Most mature mirnas in follicular cells have been found in the length of 22 nucleotides. The differential expression analysis revealed that MIRNAS were significantly regulated and 13 miRNas were significantly reduced in the Follicular Cells IIIB compared to Follicular Cells. The expression of four of the Mirnas significantly has been validated by real-time PCR.
Description: Human Large Intestine Microvascular Endothelial Cells were initiated from normal human large intestine (colon) tissue.These cells were originated using Complete Serum-Free Medium Kit, and subsequently grown and passaged in Cell Complete Medium. The cells are available at <12 Cumulative Population Doublings (CPD) in vitro at Passage 3 and were cryopreserved in aliquots of ~1.0 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75 cm^2 flask.
Human Small Intestine Microvascular Endothelial Cells
Description: Human cervix carcinoma. Originally derived from the jejunum and ileum of a 2 month old Caucasian embryo. The cells have a similar virus susceptibility as HeLa but show less sensitivity. They carry HeLa marker chromosomes.
Description: Small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data
Small intestine disease spectrum (small intestine cancer progression) tissue array
Description: Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Human small intestine tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
Finally, the enrichment and gene route analyzes of the predicted targets of the MIRNAS significantly regulated supported the involvement of several key signaling pathways in the regulation of ovarian function, including the maturation of oocytes. Taken together, this study identifies new zebra mirnas and characterizes Mirna’s expression profiles in somatic cells in zebra ovarian follicles. The differential expression of the mirnas between the follicular cells of step IIIA and IIIB suggests that these mushas are important regulatory authorities in the development of the zebrafish ovarian follicle and / or the maturation of oocytes.
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